Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i>
Carbon is one of the most important nutrients for the development and secondary metabolism in fungi. CreA is the major transcriptional factor mediating carbon catabolite repression, which is employed in the utilization of carbon sources. <i>Aspergillus ochraceus</i> contaminates various...
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MDPI AG
2020-11-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/11/697 |
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| author | Gang Wang Yulong Wang Bolei Yang Chenxi Zhang Haiyong Zhang Fuguo Xing Yang Liu |
| author_facet | Gang Wang Yulong Wang Bolei Yang Chenxi Zhang Haiyong Zhang Fuguo Xing Yang Liu |
| author_sort | Gang Wang |
| collection | DOAJ |
| description | Carbon is one of the most important nutrients for the development and secondary metabolism in fungi. CreA is the major transcriptional factor mediating carbon catabolite repression, which is employed in the utilization of carbon sources. <i>Aspergillus ochraceus</i> contaminates various food and feed containing different carbon sources by producing ochratoxin A (OTA). However, little is known about the function of <i>AoCreA</i> in regulating the morphology and OTA production of <i>A. ochraceus</i>. To give an insight into the mechanism of the carbon sources regulating development of <i>A. ochraceus</i> and OTA production, we have identified <i>AoCreA</i> in <i>A. ochraceus</i>. The homologous recombination strategy was used to generate the <i>AoCreA</i> deletion mutant (Δ<i>AoCreA</i>). We have investigated the morphology and OTA production of the wild type (WT) and Δ<i>AoCreA</i> of <i>A. ochraceus</i> with media containing different carbon sources (glucose, fructose, maltose, D-xylose, D-mannose, acetate, D-galactose, D-mannitol and lactose). Δ<i>AoCreA</i> showed a significant growth and conidiation defect on all media as compared with WT. Glucose and maltose were the most inducing media for OTA production by <i>A. ochraceus</i>, followed by sucrose and the nutrient-rich Yeast Extract Sucrose (YES) and Potato Dextrose Agar (PDA). The deletion of <i>AoCreA</i> led to a drastic reduction of OTA production on all kinds of media except PDA, which was supported by the expression profile of OTA biosynthetic genes. Furthermore, infection studies of Δ<i>AoCreA</i> on oats and pears showed the involvement of <i>AoCreA</i> in the pathogenicity of <i>A. ochraceus</i>. Thus, these results suggest that <i>AoCreA</i> regulates morphological development and OTA biosynthesis in response to carbon sources in <i>A. ochraceus</i>. |
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| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T15:07:58Z |
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| spelling | doaj.art-5b500db9a88b405ea912b868675cd8352023-11-20T19:34:43ZengMDPI AGToxins2072-66512020-11-01121169710.3390/toxins12110697Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i>Gang Wang0Yulong Wang1Bolei Yang2Chenxi Zhang3Haiyong Zhang4Fuguo Xing5Yang Liu6Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaSchool of Food Science and Engineering, Foshan University, Foshan 528231, ChinaCarbon is one of the most important nutrients for the development and secondary metabolism in fungi. CreA is the major transcriptional factor mediating carbon catabolite repression, which is employed in the utilization of carbon sources. <i>Aspergillus ochraceus</i> contaminates various food and feed containing different carbon sources by producing ochratoxin A (OTA). However, little is known about the function of <i>AoCreA</i> in regulating the morphology and OTA production of <i>A. ochraceus</i>. To give an insight into the mechanism of the carbon sources regulating development of <i>A. ochraceus</i> and OTA production, we have identified <i>AoCreA</i> in <i>A. ochraceus</i>. The homologous recombination strategy was used to generate the <i>AoCreA</i> deletion mutant (Δ<i>AoCreA</i>). We have investigated the morphology and OTA production of the wild type (WT) and Δ<i>AoCreA</i> of <i>A. ochraceus</i> with media containing different carbon sources (glucose, fructose, maltose, D-xylose, D-mannose, acetate, D-galactose, D-mannitol and lactose). Δ<i>AoCreA</i> showed a significant growth and conidiation defect on all media as compared with WT. Glucose and maltose were the most inducing media for OTA production by <i>A. ochraceus</i>, followed by sucrose and the nutrient-rich Yeast Extract Sucrose (YES) and Potato Dextrose Agar (PDA). The deletion of <i>AoCreA</i> led to a drastic reduction of OTA production on all kinds of media except PDA, which was supported by the expression profile of OTA biosynthetic genes. Furthermore, infection studies of Δ<i>AoCreA</i> on oats and pears showed the involvement of <i>AoCreA</i> in the pathogenicity of <i>A. ochraceus</i>. Thus, these results suggest that <i>AoCreA</i> regulates morphological development and OTA biosynthesis in response to carbon sources in <i>A. ochraceus</i>.https://www.mdpi.com/2072-6651/12/11/697<i>Aspergillus ochraceus</i>mycotoxincarbon sourcescarbon catabolite repressionCreAOchratoxin A |
| spellingShingle | Gang Wang Yulong Wang Bolei Yang Chenxi Zhang Haiyong Zhang Fuguo Xing Yang Liu Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i> Toxins <i>Aspergillus ochraceus</i> mycotoxin carbon sources carbon catabolite repression CreA Ochratoxin A |
| title | Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i> |
| title_full | Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i> |
| title_fullStr | Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i> |
| title_full_unstemmed | Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i> |
| title_short | Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i> |
| title_sort | carbon catabolite repression gene i aocrea i regulates morphological development and ochratoxin a biosynthesis responding to carbon sources in i aspergillus ochraceus i |
| topic | <i>Aspergillus ochraceus</i> mycotoxin carbon sources carbon catabolite repression CreA Ochratoxin A |
| url | https://www.mdpi.com/2072-6651/12/11/697 |
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