OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene Oxide
OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in <i&...
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| Format: | Article |
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MDPI AG
2021-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/5/422 |
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| author | Wenhua Tian Tao Zhang Shaohua Gu Yuyuan Guo Xiwu Gao Yongjun Zhang |
| author_facet | Wenhua Tian Tao Zhang Shaohua Gu Yuyuan Guo Xiwu Gao Yongjun Zhang |
| author_sort | Wenhua Tian |
| collection | DOAJ |
| description | OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in <i>A. lineolatus,</i> the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on <i>AlinOBP14</i>, a Plus-C OBP from <i>A. lineolatus,</i> and we developed a PNA-GO-based mRNA biosensor to detect the expression of <i>AlinOBP14</i>. The results demonstrated that <i>AlinOBP14</i> plays dual roles in <i>A. lineolatus</i>. The <i>AlinOBP14</i> is expressed beneath the epidermis of the vertex and gena in heads of <i>A. lineolatus,</i> and it functions as a carrier for three terpenoids, while <i>AlinOBP14</i> is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHⅢ. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest. |
| first_indexed | 2024-03-10T11:36:31Z |
| format | Article |
| id | doaj.art-5b502f215e1b406e9d41817fa39412cd |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T11:36:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-5b502f215e1b406e9d41817fa39412cd2023-11-21T18:49:23ZengMDPI AGInsects2075-44502021-05-0112542210.3390/insects12050422OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene OxideWenhua Tian0Tao Zhang1Shaohua Gu2Yuyuan Guo3Xiwu Gao4Yongjun Zhang5Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaOBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in <i>A. lineolatus,</i> the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on <i>AlinOBP14</i>, a Plus-C OBP from <i>A. lineolatus,</i> and we developed a PNA-GO-based mRNA biosensor to detect the expression of <i>AlinOBP14</i>. The results demonstrated that <i>AlinOBP14</i> plays dual roles in <i>A. lineolatus</i>. The <i>AlinOBP14</i> is expressed beneath the epidermis of the vertex and gena in heads of <i>A. lineolatus,</i> and it functions as a carrier for three terpenoids, while <i>AlinOBP14</i> is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHⅢ. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest.https://www.mdpi.com/2075-4450/12/5/422<i>Adelphocoris lineolatus</i><i>AlinOBP14</i>graphene oxide (GO)in vitroin vivojuvenile hormone |
| spellingShingle | Wenhua Tian Tao Zhang Shaohua Gu Yuyuan Guo Xiwu Gao Yongjun Zhang OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene Oxide Insects <i>Adelphocoris lineolatus</i> <i>AlinOBP14</i> graphene oxide (GO) in vitro in vivo juvenile hormone |
| title | OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene Oxide |
| title_full | OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene Oxide |
| title_fullStr | OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene Oxide |
| title_full_unstemmed | OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene Oxide |
| title_short | OBP14 (Odorant-Binding Protein) Sensing in <i>Adelphocoris lineolatus</i> Based on Peptide Nucleic Acid and Graphene Oxide |
| title_sort | obp14 odorant binding protein sensing in i adelphocoris lineolatus i based on peptide nucleic acid and graphene oxide |
| topic | <i>Adelphocoris lineolatus</i> <i>AlinOBP14</i> graphene oxide (GO) in vitro in vivo juvenile hormone |
| url | https://www.mdpi.com/2075-4450/12/5/422 |
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