De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model
The utility of implanting a bioscaffold mitral valve consisting of porcine small intestinal submucosa (PSIS) in a juvenile baboon model (12 to 14 months old at the time of implant; <i>n</i> = 3) to assess their in vivo tissue remodeling responses was investigated. Our findings demonstrat...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/8/7/100 |
| _version_ | 1827687865971113984 |
|---|---|
| author | Brittany A. Gonzalez Marcos Perez Gonzalez Frank Scholl Steven Bibevski Elena Ladich Jennifer Bibevski Pablo Morales Jesus Lopez Mike Casares Vincent Brehier Lazaro Hernandez Sharan Ramaswamy |
| author_facet | Brittany A. Gonzalez Marcos Perez Gonzalez Frank Scholl Steven Bibevski Elena Ladich Jennifer Bibevski Pablo Morales Jesus Lopez Mike Casares Vincent Brehier Lazaro Hernandez Sharan Ramaswamy |
| author_sort | Brittany A. Gonzalez |
| collection | DOAJ |
| description | The utility of implanting a bioscaffold mitral valve consisting of porcine small intestinal submucosa (PSIS) in a juvenile baboon model (12 to 14 months old at the time of implant; <i>n</i> = 3) to assess their in vivo tissue remodeling responses was investigated. Our findings demonstrated that the PSIS mitral valve exhibited the robust presence of de novo extracellular matrix (ECM) at all explantation time points (at 3-, 11-, and 20-months). Apart from a significantly lower level of proteoglycans in the implanted valve’s annulus region (<i>p</i> < 0.05) at 3 months compared to the 11- and 20-month explants, there were no other significant differences (<i>p</i> > 0.05) found between any of the other principal valve ECM components (collagen and elastin) at the leaflet, annulus, or chordae tendinea locations, across these time points. In particular, neochordae tissue had formed, which seamlessly integrated with the native papillary muscles. However, additional processing will be required to trigger accelerated, uniform and complete valve ECM formation in the recipient. Regardless of the specific processing done to the bioscaffold valve, in this proof-of-concept study, we estimate that a 3-month window following bioscaffold valve replacement is the timeline in which complete regeneration of the valve and integration with the host needs to occur. |
| first_indexed | 2024-03-10T09:45:54Z |
| format | Article |
| id | doaj.art-5b50a9bfdadf482791c9381c52cb05ef |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T09:45:54Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-5b50a9bfdadf482791c9381c52cb05ef2023-11-22T03:15:14ZengMDPI AGBioengineering2306-53542021-07-018710010.3390/bioengineering8070100De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate ModelBrittany A. Gonzalez0Marcos Perez Gonzalez1Frank Scholl2Steven Bibevski3Elena Ladich4Jennifer Bibevski5Pablo Morales6Jesus Lopez7Mike Casares8Vincent Brehier9Lazaro Hernandez10Sharan Ramaswamy11Department of Biomedical Engineering, Florida International University, Miami, FL 33174, USADepartment of Biomedical Engineering, Florida International University, Miami, FL 33174, USAMemorial Healthcare System, Joe DiMaggio Children’s Hospital, Hollywood, FL 33021, USADepartment of Biomedical Engineering, Florida International University, Miami, FL 33174, USAMemorial Healthcare System, Joe DiMaggio Children’s Hospital, Hollywood, FL 33021, USACOR Veterinary Surgery Services, Hollywood, FL 33301, USAThe Mannheimer Foundation, Inc., Homestead, FL 33034, USAThe Mannheimer Foundation, Inc., Homestead, FL 33034, USAMemorial Healthcare System, Joe DiMaggio Children’s Hospital, Hollywood, FL 33021, USAMemorial Healthcare System, Joe DiMaggio Children’s Hospital, Hollywood, FL 33021, USAMemorial Healthcare System, Joe DiMaggio Children’s Hospital, Hollywood, FL 33021, USADepartment of Biomedical Engineering, Florida International University, Miami, FL 33174, USAThe utility of implanting a bioscaffold mitral valve consisting of porcine small intestinal submucosa (PSIS) in a juvenile baboon model (12 to 14 months old at the time of implant; <i>n</i> = 3) to assess their in vivo tissue remodeling responses was investigated. Our findings demonstrated that the PSIS mitral valve exhibited the robust presence of de novo extracellular matrix (ECM) at all explantation time points (at 3-, 11-, and 20-months). Apart from a significantly lower level of proteoglycans in the implanted valve’s annulus region (<i>p</i> < 0.05) at 3 months compared to the 11- and 20-month explants, there were no other significant differences (<i>p</i> > 0.05) found between any of the other principal valve ECM components (collagen and elastin) at the leaflet, annulus, or chordae tendinea locations, across these time points. In particular, neochordae tissue had formed, which seamlessly integrated with the native papillary muscles. However, additional processing will be required to trigger accelerated, uniform and complete valve ECM formation in the recipient. Regardless of the specific processing done to the bioscaffold valve, in this proof-of-concept study, we estimate that a 3-month window following bioscaffold valve replacement is the timeline in which complete regeneration of the valve and integration with the host needs to occur.https://www.mdpi.com/2306-5354/8/7/100porcine small intestinal submucosa (PSIS)non-human primate modelmitral valvede novo valve tissuesspatial intensity mappingextracellular matrix |
| spellingShingle | Brittany A. Gonzalez Marcos Perez Gonzalez Frank Scholl Steven Bibevski Elena Ladich Jennifer Bibevski Pablo Morales Jesus Lopez Mike Casares Vincent Brehier Lazaro Hernandez Sharan Ramaswamy De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model Bioengineering porcine small intestinal submucosa (PSIS) non-human primate model mitral valve de novo valve tissues spatial intensity mapping extracellular matrix |
| title | De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model |
| title_full | De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model |
| title_fullStr | De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model |
| title_full_unstemmed | De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model |
| title_short | De Novo Valve Tissue Morphology Following Bioscaffold Mitral Valve Replacement in a Juvenile Non-Human Primate Model |
| title_sort | de novo valve tissue morphology following bioscaffold mitral valve replacement in a juvenile non human primate model |
| topic | porcine small intestinal submucosa (PSIS) non-human primate model mitral valve de novo valve tissues spatial intensity mapping extracellular matrix |
| url | https://www.mdpi.com/2306-5354/8/7/100 |
| work_keys_str_mv | AT brittanyagonzalez denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT marcosperezgonzalez denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT frankscholl denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT stevenbibevski denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT elenaladich denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT jenniferbibevski denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT pablomorales denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT jesuslopez denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT mikecasares denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT vincentbrehier denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT lazarohernandez denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel AT sharanramaswamy denovovalvetissuemorphologyfollowingbioscaffoldmitralvalvereplacementinajuvenilenonhumanprimatemodel |