Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity Stress
Wild common wheat species represent a significant pool of resistance genes to various environmental stresses. In this study, we examined several physiological traits and the activity of three antioxidant enzymes—namely, catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX)—as we...
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MDPI AG
2023-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/8/1981 |
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| author | Bita Jamshidi Alireza Pour-Aboughadareh Jan Bocianowski Lia Shooshtari Henryk Bujak Aras Türkoğlu Kamila Nowosad |
| author_facet | Bita Jamshidi Alireza Pour-Aboughadareh Jan Bocianowski Lia Shooshtari Henryk Bujak Aras Türkoğlu Kamila Nowosad |
| author_sort | Bita Jamshidi |
| collection | DOAJ |
| description | Wild common wheat species represent a significant pool of resistance genes to various environmental stresses. In this study, we examined several physiological traits and the activity of three antioxidant enzymes—namely, catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX)—as well as the expression patterns of their encoding genes in three neglected <i>Aegilops</i> species with alien genomes (including <i>Ae. triuncialis</i> (UUCC-genome), <i>Ae. neglecta</i> (UUMM-genome) and <i>Ae. umbellulata</i> (UU-genome)) under two control (0 mM NaCl) and salinity (250 mM NaCl) conditions. The results of the analysis of variance (ANOVA) showed highly significant effects of salinity stress, accessions, and their interaction on most physio-biochemical traits, root and shoot dry biomasses, and antioxidant-related gene expression level. As a result of comparison between <i>Aegilops</i> species and a bread wheat cultivar (cv. Narin as a salt-tolerant reference variety), <i>Ae. triuncialis</i> responded well to salinity stress, maintaining both ionic homeostasis capability and biochemical ability. Moreover, transcriptional data revealed the prominence of <i>Ae. triuncialis</i> over other <i>Aegilops</i> species and salt-tolerant bread wheat [cv. Narin] in terms of the level of expression of antioxidant genes (<i>APX</i>, <i>SOD</i>, and <i>CAT</i>). This result was further supported by a biplot rendered based on principal component analysis (PCA), where this wild relative showed a positive association with most measured traits under salinity stress. Moreover, we speculate that this accession can be subjected to physiological and molecular studies, and that it can provide new insights into the use of the alien genomes in future wheat breeding programs. |
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| id | doaj.art-5b553fcdf77440eaac8f340bed6a393d |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T00:12:25Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-5b553fcdf77440eaac8f340bed6a393d2023-11-18T23:53:25ZengMDPI AGAgronomy2073-43952023-07-01138198110.3390/agronomy13081981Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity StressBita Jamshidi0Alireza Pour-Aboughadareh1Jan Bocianowski2Lia Shooshtari3Henryk Bujak4Aras Türkoğlu5Kamila Nowosad6Department of Food Security and Public Health, Khabat Technical Institute, Erbil Polytechnic University, Erbil 44001, IraqSeed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj 31588-54119, IranDepartment of Mathematical and Statistical Methods, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, PolandDepartment of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah 67187-73654, IranDepartment of Genetics, Plant Breeding and Seed Production, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 24A, 53-535 Wrocław, PolandDepartment of Field Crops, Faculty of Agriculture, Necmettin Erbakan University, Konya 42310, TürkiyeDepartment of Genetics, Plant Breeding and Seed Production, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 24A, 53-535 Wrocław, PolandWild common wheat species represent a significant pool of resistance genes to various environmental stresses. In this study, we examined several physiological traits and the activity of three antioxidant enzymes—namely, catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX)—as well as the expression patterns of their encoding genes in three neglected <i>Aegilops</i> species with alien genomes (including <i>Ae. triuncialis</i> (UUCC-genome), <i>Ae. neglecta</i> (UUMM-genome) and <i>Ae. umbellulata</i> (UU-genome)) under two control (0 mM NaCl) and salinity (250 mM NaCl) conditions. The results of the analysis of variance (ANOVA) showed highly significant effects of salinity stress, accessions, and their interaction on most physio-biochemical traits, root and shoot dry biomasses, and antioxidant-related gene expression level. As a result of comparison between <i>Aegilops</i> species and a bread wheat cultivar (cv. Narin as a salt-tolerant reference variety), <i>Ae. triuncialis</i> responded well to salinity stress, maintaining both ionic homeostasis capability and biochemical ability. Moreover, transcriptional data revealed the prominence of <i>Ae. triuncialis</i> over other <i>Aegilops</i> species and salt-tolerant bread wheat [cv. Narin] in terms of the level of expression of antioxidant genes (<i>APX</i>, <i>SOD</i>, and <i>CAT</i>). This result was further supported by a biplot rendered based on principal component analysis (PCA), where this wild relative showed a positive association with most measured traits under salinity stress. Moreover, we speculate that this accession can be subjected to physiological and molecular studies, and that it can provide new insights into the use of the alien genomes in future wheat breeding programs.https://www.mdpi.com/2073-4395/13/8/1981ancestor specieswheatsalt tolerancebiochemical markersgene expression |
| spellingShingle | Bita Jamshidi Alireza Pour-Aboughadareh Jan Bocianowski Lia Shooshtari Henryk Bujak Aras Türkoğlu Kamila Nowosad Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity Stress Agronomy ancestor species wheat salt tolerance biochemical markers gene expression |
| title | Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity Stress |
| title_full | Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity Stress |
| title_fullStr | Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity Stress |
| title_full_unstemmed | Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity Stress |
| title_short | Analysis of Physio-Biochemical Responses and Expressional Profiling Antioxidant-Related Genes in Some Neglected <i>Aegilops</i> Species under Salinity Stress |
| title_sort | analysis of physio biochemical responses and expressional profiling antioxidant related genes in some neglected i aegilops i species under salinity stress |
| topic | ancestor species wheat salt tolerance biochemical markers gene expression |
| url | https://www.mdpi.com/2073-4395/13/8/1981 |
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