Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital
Multidrug-resistant bacteria pose a serious health threat, especially in hospitals. Horizontal gene transfer (HGT) of mobile genetic elements (MGEs) facilitates the spread of antibiotic resistance, virulence, and environmental persistence genes between nosocomial pathogens. We screened the genomes o...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/53886 |
| _version_ | 1811253247957008384 |
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| author | Daniel R Evans Marissa P Griffith Alexander J Sundermann Kathleen A Shutt Melissa I Saul Mustapha M Mustapha Jane W Marsh Vaughn S Cooper Lee H Harrison Daria Van Tyne |
| author_facet | Daniel R Evans Marissa P Griffith Alexander J Sundermann Kathleen A Shutt Melissa I Saul Mustapha M Mustapha Jane W Marsh Vaughn S Cooper Lee H Harrison Daria Van Tyne |
| author_sort | Daniel R Evans |
| collection | DOAJ |
| description | Multidrug-resistant bacteria pose a serious health threat, especially in hospitals. Horizontal gene transfer (HGT) of mobile genetic elements (MGEs) facilitates the spread of antibiotic resistance, virulence, and environmental persistence genes between nosocomial pathogens. We screened the genomes of 2173 bacterial isolates from healthcare-associated infections from a single hospital over 18 months, and identified identical nucleotide regions in bacteria belonging to distinct genera. To further resolve these shared sequences, we performed long-read sequencing on a subset of isolates and generated highly contiguous genomes. We then tracked the appearance of ten different plasmids in all 2173 genomes, and found evidence of plasmid transfer independent from bacterial transmission. Finally, we identified two instances of likely plasmid transfer within individual patients, including one plasmid that likely transferred to a second patient. This work expands our understanding of HGT in healthcare settings, and can inform efforts to limit the spread of drug-resistant pathogens in hospitals. |
| first_indexed | 2024-04-12T16:47:58Z |
| format | Article |
| id | doaj.art-5b577033a70c461ebc673ee643862f47 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:47:58Z |
| publishDate | 2020-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-5b577033a70c461ebc673ee643862f472022-12-22T03:24:31ZengeLife Sciences Publications LtdeLife2050-084X2020-04-01910.7554/eLife.53886Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospitalDaniel R Evans0Marissa P Griffith1Alexander J Sundermann2Kathleen A Shutt3Melissa I Saul4Mustapha M Mustapha5Jane W Marsh6Vaughn S Cooper7https://orcid.org/0000-0001-7726-0765Lee H Harrison8Daria Van Tyne9https://orcid.org/0000-0001-7284-0103Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, United States; Department of Infectious Diseases and Microbiology, University of Pittsburgh Graduate School of Public Health, Pittsburgh, United StatesMicrobial Genomic Epidemiology Laboratory, Infectious Diseases Epidemiology Research Unit, University of Pittsburgh School of Medicine and Graduate School of Public Health, Pittsburgh, United StatesMicrobial Genomic Epidemiology Laboratory, Infectious Diseases Epidemiology Research Unit, University of Pittsburgh School of Medicine and Graduate School of Public Health, Pittsburgh, United StatesMicrobial Genomic Epidemiology Laboratory, Infectious Diseases Epidemiology Research Unit, University of Pittsburgh School of Medicine and Graduate School of Public Health, Pittsburgh, United StatesDepartment of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, United StatesMicrobial Genomic Epidemiology Laboratory, Infectious Diseases Epidemiology Research Unit, University of Pittsburgh School of Medicine and Graduate School of Public Health, Pittsburgh, United StatesMicrobial Genomic Epidemiology Laboratory, Infectious Diseases Epidemiology Research Unit, University of Pittsburgh School of Medicine and Graduate School of Public Health, Pittsburgh, United StatesDepartment of Microbiology and Molecular Genetics, and Center for Evolutionary Biology and Medicine, University of Pittsburgh, Pittsburgh, United StatesMicrobial Genomic Epidemiology Laboratory, Infectious Diseases Epidemiology Research Unit, University of Pittsburgh School of Medicine and Graduate School of Public Health, Pittsburgh, United StatesDivision of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, United StatesMultidrug-resistant bacteria pose a serious health threat, especially in hospitals. Horizontal gene transfer (HGT) of mobile genetic elements (MGEs) facilitates the spread of antibiotic resistance, virulence, and environmental persistence genes between nosocomial pathogens. We screened the genomes of 2173 bacterial isolates from healthcare-associated infections from a single hospital over 18 months, and identified identical nucleotide regions in bacteria belonging to distinct genera. To further resolve these shared sequences, we performed long-read sequencing on a subset of isolates and generated highly contiguous genomes. We then tracked the appearance of ten different plasmids in all 2173 genomes, and found evidence of plasmid transfer independent from bacterial transmission. Finally, we identified two instances of likely plasmid transfer within individual patients, including one plasmid that likely transferred to a second patient. This work expands our understanding of HGT in healthcare settings, and can inform efforts to limit the spread of drug-resistant pathogens in hospitals.https://elifesciences.org/articles/53886antibiotic resistancecomparative genomicshorizontal gene transfergenomic epidemiology |
| spellingShingle | Daniel R Evans Marissa P Griffith Alexander J Sundermann Kathleen A Shutt Melissa I Saul Mustapha M Mustapha Jane W Marsh Vaughn S Cooper Lee H Harrison Daria Van Tyne Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital eLife antibiotic resistance comparative genomics horizontal gene transfer genomic epidemiology |
| title | Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital |
| title_full | Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital |
| title_fullStr | Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital |
| title_full_unstemmed | Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital |
| title_short | Systematic detection of horizontal gene transfer across genera among multidrug-resistant bacteria in a single hospital |
| title_sort | systematic detection of horizontal gene transfer across genera among multidrug resistant bacteria in a single hospital |
| topic | antibiotic resistance comparative genomics horizontal gene transfer genomic epidemiology |
| url | https://elifesciences.org/articles/53886 |
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