New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen
The early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2072-666X/12/10/1256 |
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| author | Pingping Xu Wenpo Feng Mei Wang Ling Zhang Gaofeng Liang Aihua Jing |
| author_facet | Pingping Xu Wenpo Feng Mei Wang Ling Zhang Gaofeng Liang Aihua Jing |
| author_sort | Pingping Xu |
| collection | DOAJ |
| description | The early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu NPs)/amine functionalized graphene oxide (NH<sub>2</sub>-GO) were the label of secondary antibodies (Ab<sub>2</sub>), and Au nanoparticle-decorated polydopamines (Au/PDA) were immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials. Dendritic tri-fan blade-like PdAuCu NPs/NH<sub>2</sub>-GO was synthesized according to a simple hydrothermal procedure and used to immobilize antibodies (Ab<sub>2</sub>) with large surfaces areas, increased catalytic properties and good adsorption to amplify the current signals. Subsequently, Ab<sub>2</sub>/PdAuCu NPs/NH<sub>2</sub>-GO catalyzed the reduction of H<sub>2</sub>O<sub>2</sub> in the sandwich-type immunoreactions. Under optimal conditions, the immunosensor exhibited a satisfactory response to CEA with a limit detection of 0.07 pg mL<sup>−1</sup> and a linear detection range from 0.1 pg mL<sup>−1</sup> to 200 ng mL<sup>−1</sup>. The proposed immunosensor could be suitable enough for a real sample analysis of CEA, and has clinical value in the early diagnosis of cancer. |
| first_indexed | 2024-03-10T06:22:00Z |
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| id | doaj.art-5b579fe44aab4565b1ecd18f867c6105 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T06:22:00Z |
| publishDate | 2021-10-01 |
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| series | Micromachines |
| spelling | doaj.art-5b579fe44aab4565b1ecd18f867c61052023-11-22T19:12:23ZengMDPI AGMicromachines2072-666X2021-10-011210125610.3390/mi12101256New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic AntigenPingping Xu0Wenpo Feng1Mei Wang2Ling Zhang3Gaofeng Liang4Aihua Jing5School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, ChinaMedical College, Pingdingshan University, Pingdingshan 467000, ChinaSchool of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, ChinaSchool of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, ChinaMedical College, Henan University of Science and Technology, Luoyang 471023, ChinaSchool of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, ChinaThe early detection of tumor markers has an effective role in the treatment of cancer. Here, a new sandwich-type electrochemical immunosensor for early label-free detection of the cancer biomarker carcinoembryonic antigen (CEA) was developed. Dendritic tri-fan blade-like PdAuCu nanoparticles (PdAuCu NPs)/amine functionalized graphene oxide (NH<sub>2</sub>-GO) were the label of secondary antibodies (Ab<sub>2</sub>), and Au nanoparticle-decorated polydopamines (Au/PDA) were immobilized on a screen-printed carbon electrode (SPCE) as the substrate materials. Dendritic tri-fan blade-like PdAuCu NPs/NH<sub>2</sub>-GO was synthesized according to a simple hydrothermal procedure and used to immobilize antibodies (Ab<sub>2</sub>) with large surfaces areas, increased catalytic properties and good adsorption to amplify the current signals. Subsequently, Ab<sub>2</sub>/PdAuCu NPs/NH<sub>2</sub>-GO catalyzed the reduction of H<sub>2</sub>O<sub>2</sub> in the sandwich-type immunoreactions. Under optimal conditions, the immunosensor exhibited a satisfactory response to CEA with a limit detection of 0.07 pg mL<sup>−1</sup> and a linear detection range from 0.1 pg mL<sup>−1</sup> to 200 ng mL<sup>−1</sup>. The proposed immunosensor could be suitable enough for a real sample analysis of CEA, and has clinical value in the early diagnosis of cancer.https://www.mdpi.com/2072-666X/12/10/1256dendritic PdAuCu nanoparticlesaminegraphene oxidesignal amplificationsandwich-type electrochemical immunosensorcarcinoembryonic antigen |
| spellingShingle | Pingping Xu Wenpo Feng Mei Wang Ling Zhang Gaofeng Liang Aihua Jing New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen Micromachines dendritic PdAuCu nanoparticles amine graphene oxide signal amplification sandwich-type electrochemical immunosensor carcinoembryonic antigen |
| title | New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen |
| title_full | New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen |
| title_fullStr | New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen |
| title_full_unstemmed | New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen |
| title_short | New Ultrasensitive Sandwich-Type Immunoassay of Dendritic Tri-Fan Blade-like PdAuCu Nanoparticles/Amine-Functionalized Graphene Oxide for Label-Free Detection of Carcinoembryonic Antigen |
| title_sort | new ultrasensitive sandwich type immunoassay of dendritic tri fan blade like pdaucu nanoparticles amine functionalized graphene oxide for label free detection of carcinoembryonic antigen |
| topic | dendritic PdAuCu nanoparticles amine graphene oxide signal amplification sandwich-type electrochemical immunosensor carcinoembryonic antigen |
| url | https://www.mdpi.com/2072-666X/12/10/1256 |
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