Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar
"nBackground: We examined a molecular method with a single-PCR for amplification of a part of CP5 gene enabling us to differen­tiate the pathogenic species, Entamoeba histolytica, from the non-pathogenic species, E. dispar. "nMethods: We developed a single PCR method fo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2010-12-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/17264.pdf&manuscript_id=17264 |
| _version_ | 1818863879062552576 |
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| author | S Rostamighalehjaghi R Jamali S Rezaie Z Babaei H Hooshyar M Rezaeian |
| author_facet | S Rostamighalehjaghi R Jamali S Rezaie Z Babaei H Hooshyar M Rezaeian |
| author_sort | S Rostamighalehjaghi |
| collection | DOAJ |
| description | "nBackground: We examined a molecular method with a single-PCR for amplification of a part of CP5 gene enabling us to differen­tiate the pathogenic species, Entamoeba histolytica, from the non-pathogenic species, E. dispar. "nMethods: We developed a single PCR method for this purpose.  After investigation of GenBank, primer pairs were de­signed from highly conserved regions of cysteine proteinase (CP5) gene. The primers were utilized in PCR using isolated ge­nomic DNA template of E. histolytica and the PCR products were then sequenced. The same primer and method for PCR was used for isolated genomic DNA template of E. dispar. "nResults: A fragment of about 950 bp was isolated in PCR by using DNA from E. histolytica, however, no banding pattern was produced by using the same primers for E. dispar. We characterized CP5 gene at molecular level in E. histolytica iso­lates from 22 positive; including 20 non-dysentery samples isolated from both cities as well as two dysentery samples iso­lated only from Tabriz. Nucleotide sequence comparison in gene data banks (NCBI, NIH) revealed significant homology with CP5 gene in E. histolytica isolates "nConclusion: We developed a PCR method, which could detect simply and rapidly E. histolytica by amplifying a specific PCR fragment. |
| first_indexed | 2024-12-19T10:22:46Z |
| format | Article |
| id | doaj.art-5b58d20fa1524596a6ae17e710848912 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 |
| language | English |
| last_indexed | 2024-12-19T10:22:46Z |
| publishDate | 2010-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-5b58d20fa1524596a6ae17e7108489122022-12-21T20:26:01ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852010-12-013946469Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. disparS RostamighalehjaghiR JamaliS RezaieZ BabaeiH HooshyarM Rezaeian"nBackground: We examined a molecular method with a single-PCR for amplification of a part of CP5 gene enabling us to differen­tiate the pathogenic species, Entamoeba histolytica, from the non-pathogenic species, E. dispar. "nMethods: We developed a single PCR method for this purpose.  After investigation of GenBank, primer pairs were de­signed from highly conserved regions of cysteine proteinase (CP5) gene. The primers were utilized in PCR using isolated ge­nomic DNA template of E. histolytica and the PCR products were then sequenced. The same primer and method for PCR was used for isolated genomic DNA template of E. dispar. "nResults: A fragment of about 950 bp was isolated in PCR by using DNA from E. histolytica, however, no banding pattern was produced by using the same primers for E. dispar. We characterized CP5 gene at molecular level in E. histolytica iso­lates from 22 positive; including 20 non-dysentery samples isolated from both cities as well as two dysentery samples iso­lated only from Tabriz. Nucleotide sequence comparison in gene data banks (NCBI, NIH) revealed significant homology with CP5 gene in E. histolytica isolates "nConclusion: We developed a PCR method, which could detect simply and rapidly E. histolytica by amplifying a specific PCR fragment.http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/17264.pdf&manuscript_id=17264AmebiasisEntamoeba disparEntamoeba histolyticaNucleic acid sequencing |
| spellingShingle | S Rostamighalehjaghi R Jamali S Rezaie Z Babaei H Hooshyar M Rezaeian Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar Iranian Journal of Public Health Amebiasis Entamoeba dispar Entamoeba histolytica Nucleic acid sequencing |
| title | Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar |
| title_full | Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar |
| title_fullStr | Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar |
| title_full_unstemmed | Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar |
| title_short | Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar |
| title_sort | evaluation of a single pcr assays on cp5 gene for differentiation of entamoeba histolytica and e dispar |
| topic | Amebiasis Entamoeba dispar Entamoeba histolytica Nucleic acid sequencing |
| url | http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/17264.pdf&manuscript_id=17264 |
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