<b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276
Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2010-10-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276 |
| _version_ | 1811230365688266752 |
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| author | Geraldo Márcio da Costa Luciano Vilela Paiva Roberta Hilsdorf Piccoli Demétrio Junqueira Figueiredo Ulisses de Pádua Pereira Nivaldo da Silva |
| author_facet | Geraldo Márcio da Costa Luciano Vilela Paiva Roberta Hilsdorf Piccoli Demétrio Junqueira Figueiredo Ulisses de Pádua Pereira Nivaldo da Silva |
| author_sort | Geraldo Márcio da Costa |
| collection | DOAJ |
| description | Evaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit (Biomériux-France), for precise identification of species. Phenotypic tests revealed 338 strains (98.25%) as S. aureus, three strains (0.86%) as Staphylococcus hyicus, and three microorganisms (0.86%) as Staphylococcus intermedius. PCR demonstrated that 338 (98.25%) strains belonged to the S. aureus species, confirming the results for 336 strains from 338 identified, through a simplified phenotypic key. A high rate of correlation (98.83%) was verifeid between the results of genotypic and phenotypic tests for the identification of S. aureus, demonstrating the applicability of the proposed key, for the discrimination of this microorganism in CPS isolated from bovine mastitis. |
| first_indexed | 2024-04-12T10:28:13Z |
| format | Article |
| id | doaj.art-5b590e56ec204c7ca79ea089fa5e40e3 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-12T10:28:13Z |
| publishDate | 2010-10-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-5b590e56ec204c7ca79ea089fa5e40e32022-12-22T03:36:55ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2010-10-0132410.4025/actascibiolsci.v32i4.6276<b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276Geraldo Márcio da Costa0Luciano Vilela Paiva1Roberta Hilsdorf Piccoli2Demétrio Junqueira Figueiredo3Ulisses de Pádua Pereira4Nivaldo da Silva5UFLAUFLAUniversidade Federal de LavrasUniversidade Federal de LavrasUFLAEV-UFMGEvaluation of a simplified key for the identification of coagulase-positive Staphylococcus isolated from bovine mastitis. Three hundred fourty four strains of coagulase-positive Staphylococcus (CPS), isolated from mastitis cases, underwent phenotypic and genotypic tests to evaluate the efficiency of a simplified key, based on phenotypic tests for the discrimination of these microorganisms. The tests consisted of amplification of the femA gene and hemolysis in blood agar, production of acetoin and fermentation of maltose, mannitol and trehalose. Strains that showed negative results in the amplification test of the femA gene or that were not identified as Staphylococcus aureus (S. aureus) by phenotypic tests were tested with the APISTAPH kit (Biomériux-France), for precise identification of species. Phenotypic tests revealed 338 strains (98.25%) as S. aureus, three strains (0.86%) as Staphylococcus hyicus, and three microorganisms (0.86%) as Staphylococcus intermedius. PCR demonstrated that 338 (98.25%) strains belonged to the S. aureus species, confirming the results for 336 strains from 338 identified, through a simplified phenotypic key. A high rate of correlation (98.83%) was verifeid between the results of genotypic and phenotypic tests for the identification of S. aureus, demonstrating the applicability of the proposed key, for the discrimination of this microorganism in CPS isolated from bovine mastitis.https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276mastitis pathogenscharacterizationcoagulase testPCRbiochemical tests |
| spellingShingle | Geraldo Márcio da Costa Luciano Vilela Paiva Roberta Hilsdorf Piccoli Demétrio Junqueira Figueiredo Ulisses de Pádua Pereira Nivaldo da Silva <b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276 Acta Scientiarum: Biological Sciences mastitis pathogens characterization coagulase test PCR biochemical tests |
| title | <b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276 |
| title_full | <b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276 |
| title_fullStr | <b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276 |
| title_full_unstemmed | <b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276 |
| title_short | <b>Evaluation of a simplified key for the identification of coagulase-positive <em>Staphylococcus</em> isolated from bovine mastitis</b> - doi: 10.4025/actascibiolsci.v32i4.6276 |
| title_sort | b evaluation of a simplified key for the identification of coagulase positive em staphylococcus em isolated from bovine mastitis b doi 10 4025 actascibiolsci v32i4 6276 |
| topic | mastitis pathogens characterization coagulase test PCR biochemical tests |
| url | https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6276 |
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