Expression of the HPV18/E6 oncoprotein induces DNA damage
<p>This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the <em>HPV/E6</em> oncogene compared with HeLa cells with normal expression of the <em>E6</em> oncogene using the DNA breakage detection–fluorescence<em> in sit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2017-06-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://ejh.it/index.php/ejh/article/view/2773 |
| Summary: | <p>This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the <em>HPV/E6</em> oncogene compared with HeLa cells with normal expression of the <em>E6</em> oncogene using the DNA breakage detection–fluorescence<em> in situ </em>hybridization (DBD–FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the <em>HPV18/E6</em> oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the <em>E6</em> oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the <em>HPV/E6</em> oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.</p> |
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| ISSN: | 1121-760X 2038-8306 |