Expression of the HPV18/E6 oncoprotein induces DNA damage
<p>This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the <em>HPV/E6</em> oncogene compared with HeLa cells with normal expression of the <em>E6</em> oncogene using the DNA breakage detection–fluorescence<em> in sit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2017-06-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://ejh.it/index.php/ejh/article/view/2773 |
| _version_ | 1811276636323053568 |
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| author | Elva I. Cortés Gutiérrez Catalina García-Vielma Adriana Aguilar-Lemarroy Veronica Vallejo-Ruíz Patricia Piña-Sánchez Pablo Zapata-Benavides Jaime Gosalvez |
| author_facet | Elva I. Cortés Gutiérrez Catalina García-Vielma Adriana Aguilar-Lemarroy Veronica Vallejo-Ruíz Patricia Piña-Sánchez Pablo Zapata-Benavides Jaime Gosalvez |
| author_sort | Elva I. Cortés Gutiérrez |
| collection | DOAJ |
| description | <p>This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the <em>HPV/E6</em> oncogene compared with HeLa cells with normal expression of the <em>E6</em> oncogene using the DNA breakage detection–fluorescence<em> in situ </em>hybridization (DBD–FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the <em>HPV18/E6</em> oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the <em>E6</em> oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the <em>HPV/E6</em> oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.</p> |
| first_indexed | 2024-04-13T00:01:18Z |
| format | Article |
| id | doaj.art-5b59fe0f88dd4414879894cf29336841 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-13T00:01:18Z |
| publishDate | 2017-06-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-5b59fe0f88dd4414879894cf293368412022-12-22T03:11:21ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062017-06-0161210.4081/ejh.2017.27731559Expression of the HPV18/E6 oncoprotein induces DNA damageElva I. Cortés Gutiérrez0Catalina García-Vielma1Adriana Aguilar-Lemarroy2Veronica Vallejo-Ruíz3Patricia Piña-Sánchez4Pablo Zapata-Benavides5Jaime Gosalvez6Instituto Mexicano del Seguro SocialInstituto Mexicano del Seguro SocialInstituto Mexicano del Seguro SocialInstituto Mexicano del Seguro SocialInstituto Mexicano del Seguro SocialInstituto Mexicano del Seguro SocialUniversidad Autónoma de Madrid<p>This study investigated possible variations in DNA damage in HeLa cells with silenced expression of the <em>HPV/E6</em> oncogene compared with HeLa cells with normal expression of the <em>E6</em> oncogene using the DNA breakage detection–fluorescence<em> in situ </em>hybridization (DBD–FISH) technique and a whole human genome DNA probe. The variable levels of DNA breaks present were measured quantitatively using image analysis after whole-genome DNA hybridization. HeLa cells with silenced expression of the <em>HPV18/E6</em> oncogene showed a significant decrease in DNA damage compared with parental cells with normal expression of the <em>E6</em> oncogene. These results were confirmed by alkaline comet assay. In conclusion, we demonstrated a decrease in DNA damage in HeLa clones associated with low expression of the <em>HPV/E6</em> oncogene. The significance of this decrease regarding the HPV life cycle and carcinogenesis requires further exploration.</p>http://ejh.it/index.php/ejh/article/view/2773DNA damageDNA breakage detection-fluorescence in situ hybridizationDBD-FISHhuman papillomavirusHPVE6 oncogenealkaline-labile sites. |
| spellingShingle | Elva I. Cortés Gutiérrez Catalina García-Vielma Adriana Aguilar-Lemarroy Veronica Vallejo-Ruíz Patricia Piña-Sánchez Pablo Zapata-Benavides Jaime Gosalvez Expression of the HPV18/E6 oncoprotein induces DNA damage European Journal of Histochemistry DNA damage DNA breakage detection-fluorescence in situ hybridization DBD-FISH human papillomavirus HPV E6 oncogene alkaline-labile sites. |
| title | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_full | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_fullStr | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_full_unstemmed | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_short | Expression of the HPV18/E6 oncoprotein induces DNA damage |
| title_sort | expression of the hpv18 e6 oncoprotein induces dna damage |
| topic | DNA damage DNA breakage detection-fluorescence in situ hybridization DBD-FISH human papillomavirus HPV E6 oncogene alkaline-labile sites. |
| url | http://ejh.it/index.php/ejh/article/view/2773 |
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