HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries)
BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic sequences of the gene. In the present study,...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/761 |
| _version_ | 1797215020102189056 |
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| author | Viorica Coşier |
| author_facet | Viorica Coşier |
| author_sort | Viorica Coşier |
| collection | DOAJ |
| description | BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic sequences of the gene. In the present study, a fragment of 356 bp, adjacent to the 2nd exon of the BMP-15 gene, was amplified by PCR in 50 individuals of Turcana and Tsigai breeds. The amplified products were subjected to digestion to identify the polymorphisms in the HinfI restriction enzyme sites. The two putative enzyme sites presented in the fragment did not reveal mutations in neither breed, releasing the same electrophoretic profile. |
| first_indexed | 2024-04-24T11:23:25Z |
| format | Article |
| id | doaj.art-5b5af63b5d9a41bdb2d33064bd98d664 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:23:25Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-5b5af63b5d9a41bdb2d33064bd98d6642024-04-11T04:04:13ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-015328080761HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries)Viorica Coşier0University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca,400372, Cluj-Napoca, Manastur str, 3-5, RomaniaBMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic sequences of the gene. In the present study, a fragment of 356 bp, adjacent to the 2nd exon of the BMP-15 gene, was amplified by PCR in 50 individuals of Turcana and Tsigai breeds. The amplified products were subjected to digestion to identify the polymorphisms in the HinfI restriction enzyme sites. The two putative enzyme sites presented in the fragment did not reveal mutations in neither breed, releasing the same electrophoretic profile.https://spasb.ro/index.php/public_html/article/view/761snppolymorphismbmp15 generestriction enzyme |
| spellingShingle | Viorica Coşier HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries) Scientific Papers Animal Science and Biotechnologies snp polymorphism bmp15 gene restriction enzyme |
| title | HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries) |
| title_full | HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries) |
| title_fullStr | HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries) |
| title_full_unstemmed | HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries) |
| title_short | HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries) |
| title_sort | hinfi polymorphisms characterization in the 1st intron of bmp15 fecx gene in turcana and tsigai sheep ovis aries |
| topic | snp polymorphism bmp15 gene restriction enzyme |
| url | https://spasb.ro/index.php/public_html/article/view/761 |
| work_keys_str_mv | AT vioricacosier hinfipolymorphismscharacterizationinthe1stintronofbmp15fecxgeneinturcanaandtsigaisheepovisaries |