A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro
The relevance of non-Saccharomyces native yeasts for improving the complexity of wine aroma is increasingly recognised, besides their use in a range of biotechnological applications. Previously, we used a metagenomic approach to assess the effects of vineyard calcium sprays on the microbiota of gra...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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International Viticulture and Enology Society
2022-08-01
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| Series: | OENO One |
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| Online Access: | https://oeno-one.eu/article/view/5462 |
| _version_ | 1811339761972936704 |
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| author | Viviana Martins Luís Costa Rafaela Soares Peter Ayogu António Teixeira Hernâni Gerós |
| author_facet | Viviana Martins Luís Costa Rafaela Soares Peter Ayogu António Teixeira Hernâni Gerós |
| author_sort | Viviana Martins |
| collection | DOAJ |
| description |
The relevance of non-Saccharomyces native yeasts for improving the complexity of wine aroma is increasingly recognised, besides their use in a range of biotechnological applications. Previously, we used a metagenomic approach to assess the effects of vineyard calcium sprays on the microbiota of grape berries cv. Vinhão and Loureiro. In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa. Seventeen unique colony morphologies were identified, and the sequencing of the region spanning the rDNA internal transcribed spacers or the rDNA D1/D2 domain revealed that they are distributed into 13 different genera. Although the cultivar and vintage influenced the taxa abundance and diversity, Aureobasidium pullulans and Hanseniaspora uvarum were the most abundant species, ubiquitously populating the fruits together with Pichia terricola, Rhodotorula glutinis, Starmerella bacillaris and Vishniacozyma heimaeyensis. The rarest genera comprised Candida, Trichosporon and Zygoascus. Analysis of yeast growth in the presence of specific elicitors revealed that Pichia spp. are the most sensitive to exogenous calcium, while Sporobolomyces roseus is the most sensitive to copper exposure. Moreover, A. pullulans, H. uvarum and S. bacillaris displayed the highest sensitivity to osmotic stress. In turn, Wickerhamomyces anomalus, Metschnikowia pulcherrima, P. fermentans, P. kluyveri and P. kudriavzevii showed mild resistance to ethanol, unlike the remaining non-Saccharomyces yeasts. Correlation networks expressing the overall yeast performance showed that H. uvarum and S. bacillaris share the strongest positive interspecies correlation, followed by the pairs P. fermentans–P. kluyveri, B. albus–R. glutinis and A. pullulans–V. heimaeyensis. The strongest negative correlations were found for the pairs P. kluyveri–S. roseus, P. terricola–W. anomalus, P. kudriavzevii–V. heimayensis, P. kluyveri–V. heimayensis, and A. pullulans–P. kluyveri. This study exposed microbial niches with a predictably common response to abiotic stress.
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| first_indexed | 2024-04-13T18:31:28Z |
| format | Article |
| id | doaj.art-5b5ee232251e46228a96935e28798255 |
| institution | Directory Open Access Journal |
| issn | 2494-1271 |
| language | English |
| last_indexed | 2024-04-13T18:31:28Z |
| publishDate | 2022-08-01 |
| publisher | International Viticulture and Enology Society |
| record_format | Article |
| series | OENO One |
| spelling | doaj.art-5b5ee232251e46228a96935e287982552022-12-22T02:35:04ZengInternational Viticulture and Enology SocietyOENO One2494-12712022-08-01563A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and LoureiroViviana Martins0Luís Costa1Rafaela Soares2Peter Ayogu3António Teixeira4Hernâni Gerós5Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, BragaCentre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, BragaCentre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, BragaCentre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, BragaCentre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, BragaCentre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, Braga The relevance of non-Saccharomyces native yeasts for improving the complexity of wine aroma is increasingly recognised, besides their use in a range of biotechnological applications. Previously, we used a metagenomic approach to assess the effects of vineyard calcium sprays on the microbiota of grape berries cv. Vinhão and Loureiro. In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa. Seventeen unique colony morphologies were identified, and the sequencing of the region spanning the rDNA internal transcribed spacers or the rDNA D1/D2 domain revealed that they are distributed into 13 different genera. Although the cultivar and vintage influenced the taxa abundance and diversity, Aureobasidium pullulans and Hanseniaspora uvarum were the most abundant species, ubiquitously populating the fruits together with Pichia terricola, Rhodotorula glutinis, Starmerella bacillaris and Vishniacozyma heimaeyensis. The rarest genera comprised Candida, Trichosporon and Zygoascus. Analysis of yeast growth in the presence of specific elicitors revealed that Pichia spp. are the most sensitive to exogenous calcium, while Sporobolomyces roseus is the most sensitive to copper exposure. Moreover, A. pullulans, H. uvarum and S. bacillaris displayed the highest sensitivity to osmotic stress. In turn, Wickerhamomyces anomalus, Metschnikowia pulcherrima, P. fermentans, P. kluyveri and P. kudriavzevii showed mild resistance to ethanol, unlike the remaining non-Saccharomyces yeasts. Correlation networks expressing the overall yeast performance showed that H. uvarum and S. bacillaris share the strongest positive interspecies correlation, followed by the pairs P. fermentans–P. kluyveri, B. albus–R. glutinis and A. pullulans–V. heimaeyensis. The strongest negative correlations were found for the pairs P. kluyveri–S. roseus, P. terricola–W. anomalus, P. kudriavzevii–V. heimayensis, P. kluyveri–V. heimayensis, and A. pullulans–P. kluyveri. This study exposed microbial niches with a predictably common response to abiotic stress. https://oeno-one.eu/article/view/5462calciumcoppercultivable yeastsinterspecies correlationsosmotic stress<i>Hanseniaspora</i> |
| spellingShingle | Viviana Martins Luís Costa Rafaela Soares Peter Ayogu António Teixeira Hernâni Gerós A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro OENO One calcium copper cultivable yeasts interspecies correlations osmotic stress <i>Hanseniaspora</i> |
| title | A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro |
| title_full | A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro |
| title_fullStr | A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro |
| title_full_unstemmed | A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro |
| title_short | A catalogue of cultivable yeasts from the microbiota of grape berries cv. Vinhão and Loureiro |
| title_sort | catalogue of cultivable yeasts from the microbiota of grape berries cv vinhao and loureiro |
| topic | calcium copper cultivable yeasts interspecies correlations osmotic stress <i>Hanseniaspora</i> |
| url | https://oeno-one.eu/article/view/5462 |
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