Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl
In vitro Regeneration of NaCl-adapted Tomato Plants (Lycopersicon esculenteum Mill.). In vitro growth seedlings of tomato (Lycopersicon esculentum Mill. Var. Sabra) were used as source of explants: internode segment and leaf fragment. These explants were cultivated into a MS-based medium containing...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Presses Agronomiques de Gembloux
2006-01-01
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| Series: | Tropicultura |
| Subjects: | |
| Online Access: | http://www.tropicultura.org/text/v24n4/221.pdf |
| _version_ | 1818523132483338240 |
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| author | Abir, M. Hannachi, C. Zid, E. |
| author_facet | Abir, M. Hannachi, C. Zid, E. |
| author_sort | Abir, M. |
| collection | DOAJ |
| description | In vitro Regeneration of NaCl-adapted Tomato Plants (Lycopersicon esculenteum Mill.). In vitro growth seedlings of tomato (Lycopersicon esculentum Mill. Var. Sabra) were used as source of explants: internode segment and leaf fragment. These explants were cultivated into a MS-based medium containing 0.2 mg.l-1ANA and 2 mg.l-1 BAP, supplemented or not with NaCl at 3 and 6 g.l-1 in order to regenerate salt-tolerant plants. Results show that internode's segment and leaf fragment formed callus both in the control and in the saline media (3 and 6 g.l-1). However NaCl affected calli growth and mineral uptake. The callus growth was stimulated by NaCl at 3 g.I-1, but reduced at 6 g.I-1. Na+ and Cl- content of calli increased in saline media with respect to the control. K+ content decreased slightly with salinity, but Ca2+ content did not change. Leaf callus showed higher K+ and Ca2+ contents both in the control and saline media and were more salt tolerant than internodes callus. Latest ones regenerated shoots only on control medium. Leaf callus adapted to 3 g.l-1 NaCl were able to regenerate shoots, but with high percentage of hyperhydricity. Regenerated plants were rooted and acclimatized successfully and they tolerated 3 g.l-1 NaCl added to irrigation solution, when cultivated on peat under glasshouse. |
| first_indexed | 2024-12-11T05:41:25Z |
| format | Article |
| id | doaj.art-5b5eec13bd9e4c829c8a813435d68983 |
| institution | Directory Open Access Journal |
| issn | 0771-3312 |
| language | English |
| last_indexed | 2024-12-11T05:41:25Z |
| publishDate | 2006-01-01 |
| publisher | Presses Agronomiques de Gembloux |
| record_format | Article |
| series | Tropicultura |
| spelling | doaj.art-5b5eec13bd9e4c829c8a813435d689832022-12-22T01:19:06ZengPresses Agronomiques de GemblouxTropicultura0771-33122006-01-01244221228Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaClAbir, M.Hannachi, C.Zid, E.In vitro Regeneration of NaCl-adapted Tomato Plants (Lycopersicon esculenteum Mill.). In vitro growth seedlings of tomato (Lycopersicon esculentum Mill. Var. Sabra) were used as source of explants: internode segment and leaf fragment. These explants were cultivated into a MS-based medium containing 0.2 mg.l-1ANA and 2 mg.l-1 BAP, supplemented or not with NaCl at 3 and 6 g.l-1 in order to regenerate salt-tolerant plants. Results show that internode's segment and leaf fragment formed callus both in the control and in the saline media (3 and 6 g.l-1). However NaCl affected calli growth and mineral uptake. The callus growth was stimulated by NaCl at 3 g.I-1, but reduced at 6 g.I-1. Na+ and Cl- content of calli increased in saline media with respect to the control. K+ content decreased slightly with salinity, but Ca2+ content did not change. Leaf callus showed higher K+ and Ca2+ contents both in the control and saline media and were more salt tolerant than internodes callus. Latest ones regenerated shoots only on control medium. Leaf callus adapted to 3 g.l-1 NaCl were able to regenerate shoots, but with high percentage of hyperhydricity. Regenerated plants were rooted and acclimatized successfully and they tolerated 3 g.l-1 NaCl added to irrigation solution, when cultivated on peat under glasshouse.http://www.tropicultura.org/text/v24n4/221.pdfTomatoIn vitro regenerationLeaf callusInternodes callusAdaptedplantSalinityTunisia |
| spellingShingle | Abir, M. Hannachi, C. Zid, E. Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl Tropicultura Tomato In vitro regeneration Leaf callus Internodes callus Adapted plant Salinity Tunisia |
| title | Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl |
| title_full | Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl |
| title_fullStr | Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl |
| title_full_unstemmed | Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl |
| title_short | Régénération in vitro de plantes de tomate Lycopersicon esculenteum Mill. adaptées au NaCl |
| title_sort | regeneration in vitro de plantes de tomate lycopersicon esculenteum mill adaptees au nacl |
| topic | Tomato In vitro regeneration Leaf callus Internodes callus Adapted plant Salinity Tunisia |
| url | http://www.tropicultura.org/text/v24n4/221.pdf |
| work_keys_str_mv | AT abirm regenerationinvitrodeplantesdetomatelycopersiconesculenteummilladapteesaunacl AT hannachic regenerationinvitrodeplantesdetomatelycopersiconesculenteummilladapteesaunacl AT zide regenerationinvitrodeplantesdetomatelycopersiconesculenteummilladapteesaunacl |