Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pylori
The Pan-Drug Resistant (PDR), Helicobacter pylori remains an intractable challenge in public health worldwide and this pathogenicity is mainly due to the presence of a cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA). On the other hand, plant extracts such as Syzygium aromaticum...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411019301191 |
| _version_ | 1811299411291013120 |
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| author | Wagih A. El-Shouny Sameh S. Ali Hegazy M. Hegazy Manar K. Abd Elnabi Asmaa Ali Jianzhong Sun |
| author_facet | Wagih A. El-Shouny Sameh S. Ali Hegazy M. Hegazy Manar K. Abd Elnabi Asmaa Ali Jianzhong Sun |
| author_sort | Wagih A. El-Shouny |
| collection | DOAJ |
| description | The Pan-Drug Resistant (PDR), Helicobacter pylori remains an intractable challenge in public health worldwide and this pathogenicity is mainly due to the presence of a cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA). On the other hand, plant extracts such as Syzygium aromaticum contain a diverse array of secondary metabolites, which could be potentially used to combat H. pylori pathogens. To our knowledge, this is the first report on the biomedical potential of S. aromaticum extract against cytotoxin-associated genes producing PDR H. pylori. In this investigation, out of 45 gastric antral biopsy specimens of dyspeptic patients, 20 strains were confirmed as H. pylori. Eight (40%) out of 20 strains were PDR H. pylori while the rest of the strains were Multi-Drug Resistant (MDR) strains. Genotypic analyses of PDR H. pylori strains showed that cagA and vacA genes were found to be 75% and 87.5%, respectively and m2s2 was the most common subtype of vacA gene. S. aromaticum showed a significant higher anti-H. pylori activity compared to that of Cinnamomum zeylanicum and Thymus vulgaris. Eugenol was the major phenolic compound (28.14%) detected in the methanolic extract of S. aromaticum. Clearly, results of the toxicological assessment confirmed the safety of S. aromaticum for use. Hence, these results suggest that S. aromaticum could be a new useful natural antimicrobial agent that could potentially combat cytotoxin genes-producing drug-resistant H. pylori. Moreover, these findings provide a scientific basis for the development of antimicrobial agents from traditional herbal medicines for gastroprotection against gastric ulcer. |
| first_indexed | 2024-04-13T06:35:28Z |
| format | Article |
| id | doaj.art-5b606f25dc5a4e909feb0bf0e6da4b10 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-04-13T06:35:28Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-5b606f25dc5a4e909feb0bf0e6da4b102022-12-22T02:57:56ZengElsevierJournal of Traditional and Complementary Medicine2225-41102020-07-01104366377Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pyloriWagih A. El-Shouny0Sameh S. Ali1Hegazy M. Hegazy2Manar K. Abd Elnabi3Asmaa Ali4Jianzhong Sun5Botany Department, Faculty of Science, Tanta University, Tanta, 31527, EgyptBiofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China; Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt; Corresponding author. Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, 212013, Zhenjiang, China.Department of Internal Medicine, Faculty of Medicine, Tanta University, Tanta, 31527, EgyptBotany Department, Faculty of Science, Tanta University, Tanta, 31527, EgyptMinistry of Health and Population, Chest Directorate Abbassia Chest Hospital, Cairo, EgyptBiofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China; Corresponding author. Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, 212013, Zhenjiang, China.The Pan-Drug Resistant (PDR), Helicobacter pylori remains an intractable challenge in public health worldwide and this pathogenicity is mainly due to the presence of a cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA). On the other hand, plant extracts such as Syzygium aromaticum contain a diverse array of secondary metabolites, which could be potentially used to combat H. pylori pathogens. To our knowledge, this is the first report on the biomedical potential of S. aromaticum extract against cytotoxin-associated genes producing PDR H. pylori. In this investigation, out of 45 gastric antral biopsy specimens of dyspeptic patients, 20 strains were confirmed as H. pylori. Eight (40%) out of 20 strains were PDR H. pylori while the rest of the strains were Multi-Drug Resistant (MDR) strains. Genotypic analyses of PDR H. pylori strains showed that cagA and vacA genes were found to be 75% and 87.5%, respectively and m2s2 was the most common subtype of vacA gene. S. aromaticum showed a significant higher anti-H. pylori activity compared to that of Cinnamomum zeylanicum and Thymus vulgaris. Eugenol was the major phenolic compound (28.14%) detected in the methanolic extract of S. aromaticum. Clearly, results of the toxicological assessment confirmed the safety of S. aromaticum for use. Hence, these results suggest that S. aromaticum could be a new useful natural antimicrobial agent that could potentially combat cytotoxin genes-producing drug-resistant H. pylori. Moreover, these findings provide a scientific basis for the development of antimicrobial agents from traditional herbal medicines for gastroprotection against gastric ulcer.http://www.sciencedirect.com/science/article/pii/S2225411019301191Helicobacter pyloriDrug resistanceCytotoxin-associated genesSyzygium aromaticumHistopathologyAntibacterial |
| spellingShingle | Wagih A. El-Shouny Sameh S. Ali Hegazy M. Hegazy Manar K. Abd Elnabi Asmaa Ali Jianzhong Sun Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pylori Journal of Traditional and Complementary Medicine Helicobacter pylori Drug resistance Cytotoxin-associated genes Syzygium aromaticum Histopathology Antibacterial |
| title | Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pylori |
| title_full | Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pylori |
| title_fullStr | Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pylori |
| title_full_unstemmed | Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pylori |
| title_short | Syzygium aromaticum L.: Traditional herbal medicine against cagA and vacA toxin genes-producing drug resistant Helicobacter pylori |
| title_sort | syzygium aromaticum l traditional herbal medicine against caga and vaca toxin genes producing drug resistant helicobacter pylori |
| topic | Helicobacter pylori Drug resistance Cytotoxin-associated genes Syzygium aromaticum Histopathology Antibacterial |
| url | http://www.sciencedirect.com/science/article/pii/S2225411019301191 |
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