A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed
Abstract Background Storage starch in starchy seed influences the seed weight and texture, and determines its applications in food and nonfood industries. Starch granules from different plant sources have significantly different shapes and sizes, and even more the difference exists in the different...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-02-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13007-018-0283-x |
| _version_ | 1819014192559030272 |
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| author | Lingxiao Zhao Ting Pan Dongwei Guo Cunxu Wei |
| author_facet | Lingxiao Zhao Ting Pan Dongwei Guo Cunxu Wei |
| author_sort | Lingxiao Zhao |
| collection | DOAJ |
| description | Abstract Background Storage starch in starchy seed influences the seed weight and texture, and determines its applications in food and nonfood industries. Starch granules from different plant sources have significantly different shapes and sizes, and even more the difference exists in the different regions of the same tissue. Therefore, it is very important to in situ investigate the morphology and distribution of starch in the whole seed. However, a simple and rapid method is deficient to prepare the whole section of starchy seed for investigating the morphology and distribution of starch in the whole seeds for a large number of samples. Results A simple and rapid method was established to prepare the whole section of starchy seed, especially for floury seed, in this study. The whole seeds of translucent and chalky rice, vitreous and floury maize, and normal barley and wheat were sectioned successfully using the newly established method. The iodine-stained section clearly exhibited the shapes and size of starch granules in different regions of seed. The starch granules with different morphologies and iodine-staining colors existed regionally in the seeds of high-amylose rice and maize. The sections of lotus and kidney bean seeds also showed the feasibility of this method for starchy non-cereal seeds. Conclusion The simple and rapid method was proven effective for preparing the whole sections of starchy seeds. The whole section of seed could be used to investigate the morphology and distribution of starch granules in different regions of the whole seed. The method was especially suitable for large sample numbers to investigate the starch morphology in short time. |
| first_indexed | 2024-12-21T02:11:56Z |
| format | Article |
| id | doaj.art-5b60b160aa894913a665a2beb3b8cda9 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-12-21T02:11:56Z |
| publishDate | 2018-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-5b60b160aa894913a665a2beb3b8cda92022-12-21T19:19:20ZengBMCPlant Methods1746-48112018-02-0114111110.1186/s13007-018-0283-xA simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seedLingxiao Zhao0Ting Pan1Dongwei Guo2Cunxu Wei3Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityKey Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityMaize Biology and Genetic Laboratory in Northwest Arid Area in China, Ministry of Agriculture, Northwest A & F UniversityKey Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou UniversityAbstract Background Storage starch in starchy seed influences the seed weight and texture, and determines its applications in food and nonfood industries. Starch granules from different plant sources have significantly different shapes and sizes, and even more the difference exists in the different regions of the same tissue. Therefore, it is very important to in situ investigate the morphology and distribution of starch in the whole seed. However, a simple and rapid method is deficient to prepare the whole section of starchy seed for investigating the morphology and distribution of starch in the whole seeds for a large number of samples. Results A simple and rapid method was established to prepare the whole section of starchy seed, especially for floury seed, in this study. The whole seeds of translucent and chalky rice, vitreous and floury maize, and normal barley and wheat were sectioned successfully using the newly established method. The iodine-stained section clearly exhibited the shapes and size of starch granules in different regions of seed. The starch granules with different morphologies and iodine-staining colors existed regionally in the seeds of high-amylose rice and maize. The sections of lotus and kidney bean seeds also showed the feasibility of this method for starchy non-cereal seeds. Conclusion The simple and rapid method was proven effective for preparing the whole sections of starchy seeds. The whole section of seed could be used to investigate the morphology and distribution of starch granules in different regions of the whole seed. The method was especially suitable for large sample numbers to investigate the starch morphology in short time.http://link.springer.com/article/10.1186/s13007-018-0283-xStarch granuleMorphologyDistributionStarchy seedWhole section of seed |
| spellingShingle | Lingxiao Zhao Ting Pan Dongwei Guo Cunxu Wei A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed Plant Methods Starch granule Morphology Distribution Starchy seed Whole section of seed |
| title | A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed |
| title_full | A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed |
| title_fullStr | A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed |
| title_full_unstemmed | A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed |
| title_short | A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed |
| title_sort | simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed |
| topic | Starch granule Morphology Distribution Starchy seed Whole section of seed |
| url | http://link.springer.com/article/10.1186/s13007-018-0283-x |
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