Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages
This paper describes a procedure for the separation of lecithins according to the number of ethylenic bonds in their fatty acid residues. The procedure uses a column of alkylated dextran (Sephadex LH-20) eluted with an organic solvent system, the unsaturated lipids being separated as their mercuric...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1970-07-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520429773 |
| _version_ | 1818941188650041344 |
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| author | Richard J. King John A. Clements |
| author_facet | Richard J. King John A. Clements |
| author_sort | Richard J. King |
| collection | DOAJ |
| description | This paper describes a procedure for the separation of lecithins according to the number of ethylenic bonds in their fatty acid residues. The procedure uses a column of alkylated dextran (Sephadex LH-20) eluted with an organic solvent system, the unsaturated lipids being separated as their mercuric acetate addition compounds. The system is capable of resolving at least four species of lecithin, and the intact lecithin molecules can be recovered for further study. The chromatographic system has been tested with lecithin derived from dog lung, rat liver, and hen's egg. |
| first_indexed | 2024-12-20T06:51:34Z |
| format | Article |
| id | doaj.art-5b60c404de0741d391cda6df3b11121b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T06:51:34Z |
| publishDate | 1970-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-5b60c404de0741d391cda6df3b11121b2022-12-21T19:49:31ZengElsevierJournal of Lipid Research0022-22751970-07-01114381385Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkagesRichard J. King0John A. Clements1Cardiovascular Research Institute, University of California, San Francisco Medical Center, San Francisco, California 94122Cardiovascular Research Institute, University of California, San Francisco Medical Center, San Francisco, California 94122This paper describes a procedure for the separation of lecithins according to the number of ethylenic bonds in their fatty acid residues. The procedure uses a column of alkylated dextran (Sephadex LH-20) eluted with an organic solvent system, the unsaturated lipids being separated as their mercuric acetate addition compounds. The system is capable of resolving at least four species of lecithin, and the intact lecithin molecules can be recovered for further study. The chromatographic system has been tested with lecithin derived from dog lung, rat liver, and hen's egg.http://www.sciencedirect.com/science/article/pii/S0022227520429773alveolar surface active materialSephadex LH-20phosphatidylcholine chromatography |
| spellingShingle | Richard J. King John A. Clements Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages Journal of Lipid Research alveolar surface active material Sephadex LH-20 phosphatidylcholine chromatography |
| title | Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages |
| title_full | Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages |
| title_fullStr | Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages |
| title_full_unstemmed | Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages |
| title_short | Separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages |
| title_sort | separation by gel chromatography of naturally occurring phosphatidylcholine mixtures according to number of ethylenic linkages |
| topic | alveolar surface active material Sephadex LH-20 phosphatidylcholine chromatography |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520429773 |
| work_keys_str_mv | AT richardjking separationbygelchromatographyofnaturallyoccurringphosphatidylcholinemixturesaccordingtonumberofethyleniclinkages AT johnaclements separationbygelchromatographyofnaturallyoccurringphosphatidylcholinemixturesaccordingtonumberofethyleniclinkages |