The effects of valproic acid on skin healing: experimental study in rats
ABSTRACT Purpose: To recognize the effects of valproic acid (VPA), an epigenetic drug, on the skin healing process. Methods: Sixty male Wistar rats were divided into two groups: the experiment treated with VPA (100 mg/kg/day); and the control, with 0.9% sodium chloride by gavage. Skin healing was...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-07-01
|
| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000400205&tlng=en |
| _version_ | 1811295352283725824 |
|---|---|
| author | Rachel Biondo-Simões Maria de Lourdes Pessole Biondo-Simões Sérgio Ossamu Ioshii Rogério Ribeiro Robes Moacir de Oliveira Dall’Antonia |
| author_facet | Rachel Biondo-Simões Maria de Lourdes Pessole Biondo-Simões Sérgio Ossamu Ioshii Rogério Ribeiro Robes Moacir de Oliveira Dall’Antonia |
| author_sort | Rachel Biondo-Simões |
| collection | DOAJ |
| description | ABSTRACT Purpose: To recognize the effects of valproic acid (VPA), an epigenetic drug, on the skin healing process. Methods: Sixty male Wistar rats were divided into two groups: the experiment treated with VPA (100 mg/kg/day); and the control, with 0.9% sodium chloride by gavage. Skin healing was studied in three moments (the third, the seventh, and the 14th day), evaluating the parameters: inflammatory reaction and its intensity (anti-LCA), angiogenesis (anti-CD34), collagen I and III (anti-collagen I, anti-collagen III and Picrosirius-red F3BA) and myofibroblasts (anti-alpha-AMS). Results: The inflammatory reaction was acute or sub-acute in both groups on the third day. On the seventh and the 14th day, chronic predominated in the control (p=0.006), and sub-acute in the experiment (p=0.020). There was a greater number of leukocytes in the group treated only on the third day (p=0.036). The number of vessels was lower in the treated group at the three times (p3=0.002, p7<0.001, and p14=0.027). Myofibroblasts were rare in the third day and moderate quantity in the remaining periods. Collagen I density was higher in the control at the three times (p<0.001) and collagen III in the treated group (p<0.001). Conclusions: VPA led to a more intense inflammatory reaction, decreased angiogenesis and collagen deposition, especially type I collagen. |
| first_indexed | 2024-04-13T05:32:01Z |
| format | Article |
| id | doaj.art-5b63f5135b154000ab43cf73db889ff8 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-13T05:32:01Z |
| publishDate | 2022-07-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-5b63f5135b154000ab43cf73db889ff82022-12-22T03:00:24ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-07-0137410.1590/acb370403The effects of valproic acid on skin healing: experimental study in ratsRachel Biondo-Simõeshttps://orcid.org/0000-0002-2129-3848Maria de Lourdes Pessole Biondo-Simõeshttps://orcid.org/0000-0001-7056-8142Sérgio Ossamu Ioshiihttps://orcid.org/0000-0002-7871-4463Rogério Ribeiro Robeshttps://orcid.org/0000-0002-3598-6370Moacir de Oliveira Dall’Antoniahttps://orcid.org/0000-0002-4937-1801ABSTRACT Purpose: To recognize the effects of valproic acid (VPA), an epigenetic drug, on the skin healing process. Methods: Sixty male Wistar rats were divided into two groups: the experiment treated with VPA (100 mg/kg/day); and the control, with 0.9% sodium chloride by gavage. Skin healing was studied in three moments (the third, the seventh, and the 14th day), evaluating the parameters: inflammatory reaction and its intensity (anti-LCA), angiogenesis (anti-CD34), collagen I and III (anti-collagen I, anti-collagen III and Picrosirius-red F3BA) and myofibroblasts (anti-alpha-AMS). Results: The inflammatory reaction was acute or sub-acute in both groups on the third day. On the seventh and the 14th day, chronic predominated in the control (p=0.006), and sub-acute in the experiment (p=0.020). There was a greater number of leukocytes in the group treated only on the third day (p=0.036). The number of vessels was lower in the treated group at the three times (p3=0.002, p7<0.001, and p14=0.027). Myofibroblasts were rare in the third day and moderate quantity in the remaining periods. Collagen I density was higher in the control at the three times (p<0.001) and collagen III in the treated group (p<0.001). Conclusions: VPA led to a more intense inflammatory reaction, decreased angiogenesis and collagen deposition, especially type I collagen.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000400205&tlng=enValproic AcidEpigenesis, GeneticWound HealingCell ProliferationCollagen |
| spellingShingle | Rachel Biondo-Simões Maria de Lourdes Pessole Biondo-Simões Sérgio Ossamu Ioshii Rogério Ribeiro Robes Moacir de Oliveira Dall’Antonia The effects of valproic acid on skin healing: experimental study in rats Acta Cirúrgica Brasileira Valproic Acid Epigenesis, Genetic Wound Healing Cell Proliferation Collagen |
| title | The effects of valproic acid on skin healing: experimental study in rats |
| title_full | The effects of valproic acid on skin healing: experimental study in rats |
| title_fullStr | The effects of valproic acid on skin healing: experimental study in rats |
| title_full_unstemmed | The effects of valproic acid on skin healing: experimental study in rats |
| title_short | The effects of valproic acid on skin healing: experimental study in rats |
| title_sort | effects of valproic acid on skin healing experimental study in rats |
| topic | Valproic Acid Epigenesis, Genetic Wound Healing Cell Proliferation Collagen |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000400205&tlng=en |
| work_keys_str_mv | AT rachelbiondosimoes theeffectsofvalproicacidonskinhealingexperimentalstudyinrats AT mariadelourdespessolebiondosimoes theeffectsofvalproicacidonskinhealingexperimentalstudyinrats AT sergioossamuioshii theeffectsofvalproicacidonskinhealingexperimentalstudyinrats AT rogerioribeirorobes theeffectsofvalproicacidonskinhealingexperimentalstudyinrats AT moacirdeoliveiradallantonia theeffectsofvalproicacidonskinhealingexperimentalstudyinrats AT rachelbiondosimoes effectsofvalproicacidonskinhealingexperimentalstudyinrats AT mariadelourdespessolebiondosimoes effectsofvalproicacidonskinhealingexperimentalstudyinrats AT sergioossamuioshii effectsofvalproicacidonskinhealingexperimentalstudyinrats AT rogerioribeirorobes effectsofvalproicacidonskinhealingexperimentalstudyinrats AT moacirdeoliveiradallantonia effectsofvalproicacidonskinhealingexperimentalstudyinrats |