Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density
ABSTRACT: The high stocking density is a major stress factor that adversely affects the health and performance of poultry. Therefore, the object of this study was conducted to explore whether dietary 25-hydroxyvitamin D (25-OH-D3) could improve gut health of laying hens reared under high stocking de...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121001668 |
| _version_ | 1818892544043384832 |
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| author | Jianping Wang Chunhua Zhang Tao Zhang Lei Yan Lingyun Qiu Huadong Yin Xuemei Ding Shiping Bai Qiufeng Zeng Xiangbing Mao Keying Zhang Caimei Wu Yue Xuan Zhiguo Shan |
| author_facet | Jianping Wang Chunhua Zhang Tao Zhang Lei Yan Lingyun Qiu Huadong Yin Xuemei Ding Shiping Bai Qiufeng Zeng Xiangbing Mao Keying Zhang Caimei Wu Yue Xuan Zhiguo Shan |
| author_sort | Jianping Wang |
| collection | DOAJ |
| description | ABSTRACT: The high stocking density is a major stress factor that adversely affects the health and performance of poultry. Therefore, the object of this study was conducted to explore whether dietary 25-hydroxyvitamin D (25-OH-D3) could improve gut health of laying hens reared under high stocking density. A 2 × 2 factorial design was used in this 16-week study, in which 800 45-week-old Lohmann laying hens were allocated into two levels of dietary 25-OH-D3 levels (0 and 69 µg/kg) and two rates of stocking densities [506 (low density, LD) and 338 (high density, HD) cm2/hen]. Compared with the layers with LD, the layers with HD had lower crypt depth in duodenum (P(Density) < 0.05), lower short chain fatty acid (propionic and butyric acid) contents in cecum (P(Density) < 0.05), and lower mRNA expression of intestinal barrier associated protein (claudin-1, mucin-1 and mucin-2). Exposed layer to HD also led to lower intestinal antioxidative capacity [superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), and higher malondialdehyde (MDA) content] in small intestine (P(Density) < 0.05), lower bacterial abundance of Bacteroidetes (phylum), Spirochaetes (phylum) and Bacteroides (genus; P(Density) < 0.05), higher bacterial enrichment of Lactobacillaceae (genus) and Firmicutes/Bacteroidetes ratio (P(Density) < 0.05) in cecum. Dietary 25-OH-D3 increased the villus height in duodenum and jejunum (P(25-OH-D3) < 0.05), decreased Chao 1 and ACE indexes in cecum (P(25-OH-D3) < 0.05), and it also up-regulated the mRNA expression of claudin-1, mucin-1 and mucin-2 (P(25-OH-D3) < 0.05). Layers treated with 25-OH-D3 led to an enhanced antioxidative enzyme activity of CAT (P(25-OH-D3) < 0.05). Additionally, the effect of 25-OH-D3 reversed the effect of HD on T-AOC and MDA content (P(Interaction) < 0.05). In HD layers, 25-OH-D3 administration decreased the enrichment of Bacteroidetes (phylum), increased Firmicutes (phylum), and Firmicutes/Bacteroidetes ratio (P(Interaction) < 0.05). These results suggest that supplementing 25-OH-D3 in diets may elevate gut health through the improvement of intestinal barrier function, antioxidant capacity and cecal microbiota composition in laying hens with high stocking density. |
| first_indexed | 2024-12-19T17:58:23Z |
| format | Article |
| id | doaj.art-5b651666119d46fe806fd9fa58a3380d |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-19T17:58:23Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-5b651666119d46fe806fd9fa58a3380d2022-12-21T20:11:44ZengElsevierPoultry Science0032-57912021-07-011007101132Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking densityJianping Wang0Chunhua Zhang1Tao Zhang2Lei Yan3Lingyun Qiu4Huadong Yin5Xuemei Ding6Shiping Bai7Qiufeng Zeng8Xiangbing Mao9Keying Zhang10Caimei Wu11Yue Xuan12Zhiguo Shan13Animal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Agriculture and Forestry, Pu'er University, Pu'er City, 665000, P.R. ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaDSM Nutrition Product, DSM Vitamin (Shanghai) Ltd. Shanghai, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, ChinaAnimal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China; College of Agriculture and Forestry, Pu'er University, Pu'er City, 665000, P.R. China; Corresponding author:ABSTRACT: The high stocking density is a major stress factor that adversely affects the health and performance of poultry. Therefore, the object of this study was conducted to explore whether dietary 25-hydroxyvitamin D (25-OH-D3) could improve gut health of laying hens reared under high stocking density. A 2 × 2 factorial design was used in this 16-week study, in which 800 45-week-old Lohmann laying hens were allocated into two levels of dietary 25-OH-D3 levels (0 and 69 µg/kg) and two rates of stocking densities [506 (low density, LD) and 338 (high density, HD) cm2/hen]. Compared with the layers with LD, the layers with HD had lower crypt depth in duodenum (P(Density) < 0.05), lower short chain fatty acid (propionic and butyric acid) contents in cecum (P(Density) < 0.05), and lower mRNA expression of intestinal barrier associated protein (claudin-1, mucin-1 and mucin-2). Exposed layer to HD also led to lower intestinal antioxidative capacity [superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), and higher malondialdehyde (MDA) content] in small intestine (P(Density) < 0.05), lower bacterial abundance of Bacteroidetes (phylum), Spirochaetes (phylum) and Bacteroides (genus; P(Density) < 0.05), higher bacterial enrichment of Lactobacillaceae (genus) and Firmicutes/Bacteroidetes ratio (P(Density) < 0.05) in cecum. Dietary 25-OH-D3 increased the villus height in duodenum and jejunum (P(25-OH-D3) < 0.05), decreased Chao 1 and ACE indexes in cecum (P(25-OH-D3) < 0.05), and it also up-regulated the mRNA expression of claudin-1, mucin-1 and mucin-2 (P(25-OH-D3) < 0.05). Layers treated with 25-OH-D3 led to an enhanced antioxidative enzyme activity of CAT (P(25-OH-D3) < 0.05). Additionally, the effect of 25-OH-D3 reversed the effect of HD on T-AOC and MDA content (P(Interaction) < 0.05). In HD layers, 25-OH-D3 administration decreased the enrichment of Bacteroidetes (phylum), increased Firmicutes (phylum), and Firmicutes/Bacteroidetes ratio (P(Interaction) < 0.05). These results suggest that supplementing 25-OH-D3 in diets may elevate gut health through the improvement of intestinal barrier function, antioxidant capacity and cecal microbiota composition in laying hens with high stocking density.http://www.sciencedirect.com/science/article/pii/S003257912100166825-hydroxycholecalciferolstocking densityintestinal barrierantioxidative capacitymicrobiota |
| spellingShingle | Jianping Wang Chunhua Zhang Tao Zhang Lei Yan Lingyun Qiu Huadong Yin Xuemei Ding Shiping Bai Qiufeng Zeng Xiangbing Mao Keying Zhang Caimei Wu Yue Xuan Zhiguo Shan Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density Poultry Science 25-hydroxycholecalciferol stocking density intestinal barrier antioxidative capacity microbiota |
| title | Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density |
| title_full | Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density |
| title_fullStr | Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density |
| title_full_unstemmed | Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density |
| title_short | Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density |
| title_sort | dietary 25 hydroxyvitamin d improves intestinal health and microbiota of laying hens under high stocking density |
| topic | 25-hydroxycholecalciferol stocking density intestinal barrier antioxidative capacity microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121001668 |
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