Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategy
Abstract Background Jilin white goose is an excellent local breed in China, with a high annual egg production and laying eggs mainly from February to July each year. The testis, as the only organ that can produce sperm, can affect the sexual maturity and fecundity of male animals. Its growth and dev...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-024-10166-9 |
| _version_ | 1797219999228624896 |
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| author | Yupu Song Jingyun Ma Qiuyuan Liu Ichraf Mabrouk Yuxuan Zhou Jin Yu Fengshuo Liu Jingbo Wang Zhiye Yu Jingtao Hu Yongfeng Sun |
| author_facet | Yupu Song Jingyun Ma Qiuyuan Liu Ichraf Mabrouk Yuxuan Zhou Jin Yu Fengshuo Liu Jingbo Wang Zhiye Yu Jingtao Hu Yongfeng Sun |
| author_sort | Yupu Song |
| collection | DOAJ |
| description | Abstract Background Jilin white goose is an excellent local breed in China, with a high annual egg production and laying eggs mainly from February to July each year. The testis, as the only organ that can produce sperm, can affect the sexual maturity and fecundity of male animals. Its growth and development are affected and regulated by a variety of factors. Proteomics is generally applied to identify and quantify proteins in cells and tissues in order to understand the physiological or pathological changes that occur in tissues or cells under specific conditions. Currently, the female poultry reproductive system has been extensively studied, while few related studies focusing on the regulatory mechanism of the reproductive system of male poultry have been conducted. Results A total of 1753 differentially expressed proteins (DEPs) were generated in which there were 594, 391 and 768 different proteins showing differential expression in three stages, Initial of Laying Cycle (ILC), Peak of Laying Cycle (PLC) and End of Laying Cycle (ELC). Furthermore, bioinformatics was used to analyze the DEPs. Gene ontology (GO) enrichment, Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) network analysis were adopted. All DEPs were found to be implicated in multiple biological processes and pathways associated with testicular development, such as renin secretion, Lysosomes, SNARE interactions in vesicle trafficking, the p53 signaling pathway and pathways related to metabolism. Additionally, the reliability of transcriptome results was verified by real-time quantitative PCR by selecting the transcript abundance of 6 selected DEPs at the three stages of the laying cycle. Conclusions The funding in this study will provide critical insight into the complex molecular mechanisms and breeding practices underlying the developmental characteristics of testicles in Jilin white goose. |
| first_indexed | 2024-04-24T12:42:34Z |
| format | Article |
| id | doaj.art-5b6596902f7744edb2139c40ec874c79 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-24T12:42:34Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-5b6596902f7744edb2139c40ec874c792024-04-07T11:09:10ZengBMCBMC Genomics1471-21642024-04-0125111110.1186/s12864-024-10166-9Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategyYupu Song0Jingyun Ma1Qiuyuan Liu2Ichraf Mabrouk3Yuxuan Zhou4Jin Yu5Fengshuo Liu6Jingbo Wang7Zhiye Yu8Jingtao Hu9Yongfeng Sun10College of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityCollege of Animal Science and Technology, Jilin Agricultural UniversityAbstract Background Jilin white goose is an excellent local breed in China, with a high annual egg production and laying eggs mainly from February to July each year. The testis, as the only organ that can produce sperm, can affect the sexual maturity and fecundity of male animals. Its growth and development are affected and regulated by a variety of factors. Proteomics is generally applied to identify and quantify proteins in cells and tissues in order to understand the physiological or pathological changes that occur in tissues or cells under specific conditions. Currently, the female poultry reproductive system has been extensively studied, while few related studies focusing on the regulatory mechanism of the reproductive system of male poultry have been conducted. Results A total of 1753 differentially expressed proteins (DEPs) were generated in which there were 594, 391 and 768 different proteins showing differential expression in three stages, Initial of Laying Cycle (ILC), Peak of Laying Cycle (PLC) and End of Laying Cycle (ELC). Furthermore, bioinformatics was used to analyze the DEPs. Gene ontology (GO) enrichment, Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction (PPI) network analysis were adopted. All DEPs were found to be implicated in multiple biological processes and pathways associated with testicular development, such as renin secretion, Lysosomes, SNARE interactions in vesicle trafficking, the p53 signaling pathway and pathways related to metabolism. Additionally, the reliability of transcriptome results was verified by real-time quantitative PCR by selecting the transcript abundance of 6 selected DEPs at the three stages of the laying cycle. Conclusions The funding in this study will provide critical insight into the complex molecular mechanisms and breeding practices underlying the developmental characteristics of testicles in Jilin white goose.https://doi.org/10.1186/s12864-024-10166-9Jilin white gooseTestisProteomicsPoultrySperm |
| spellingShingle | Yupu Song Jingyun Ma Qiuyuan Liu Ichraf Mabrouk Yuxuan Zhou Jin Yu Fengshuo Liu Jingbo Wang Zhiye Yu Jingtao Hu Yongfeng Sun Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategy BMC Genomics Jilin white goose Testis Proteomics Poultry Sperm |
| title | Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategy |
| title_full | Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategy |
| title_fullStr | Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategy |
| title_full_unstemmed | Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategy |
| title_short | Protein profile analysis of Jilin white goose testicles at different stages of the laying cycle by DIA strategy |
| title_sort | protein profile analysis of jilin white goose testicles at different stages of the laying cycle by dia strategy |
| topic | Jilin white goose Testis Proteomics Poultry Sperm |
| url | https://doi.org/10.1186/s12864-024-10166-9 |
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