Sediment bioelectrochemical system with potential application against vibriosis in aquaculture
Aquaculture can suffer from heavy losses caused by vibriosis, which denotes diseases caused by Vibrio bacteria. Due to disadvantages of available measures, mostly involving the use of antibiotics, to control these bacteria, novel measures are still needed. Therefore, aiming at an innovative solution...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003654 |
| _version_ | 1797388810235936768 |
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| author | Phuong Ha Vu Nhung Hong Tran Thuy Thu Thi Nguyen Hanh My Tran Ha Viet Thi Bui Huy Quang Nguyen Thao Kim Nu Nguyen Hai The Pham |
| author_facet | Phuong Ha Vu Nhung Hong Tran Thuy Thu Thi Nguyen Hanh My Tran Ha Viet Thi Bui Huy Quang Nguyen Thao Kim Nu Nguyen Hai The Pham |
| author_sort | Phuong Ha Vu |
| collection | DOAJ |
| description | Aquaculture can suffer from heavy losses caused by vibriosis, which denotes diseases caused by Vibrio bacteria. Due to disadvantages of available measures, mostly involving the use of antibiotics, to control these bacteria, novel measures are still needed. Therefore, aiming at an innovative solution for that issue in a long term, we investigated the inhibitory effects of the sediment bioelectrochemical system (SBES) on a V. harveyi strain (Vh) and a V. parahaemolyticus strain (Vp). SBESs were installed in test pond models that were lab-scale plastic tanks containing artificial brackish water, while the resembling systems not having SBESs were used as the controls. Once the SBES stably generated electricity (at ca. 0.5 mA), the tests with the Vibrio strains were conducted by: (i) supplementing the Vibrio cell suspensions directly into the tank waters, (ii) immersing the Vibrio cells suspensions each in an isolated axenic space into the tank environments, and (iii) adding the filtered tank waters after taken out of the tanks to the Vibrio cells. Based on plate enumeration to evaluate the inhibitory effects of such treatments on the Vibrio strains, we discovered that almost 100% of both Vh and Vp cells could not grow after 5 min in contact with the SBES water while more than 80% of them could still grow after 1–2 h in contact with the water from the control tanks. Paralleling tests showed that the SBES water was also toxic to an Escherichia coli strain but only slightly inhibited a Bacillus pumilus strain and a Lactobacillus plantarum strain, reducing their growths by only about 60% and 30%, respectively. Further tests revealed that the effects were not due to suspected proteinacous components or metal contents or peroxides in the SBES water. On the other hand, applying a redox potential of 20 mV (vs. Ag/AgCl), which was the anode potential measured in the SBES, onto a graphite rod immersed in Vh cell suspension led to the growth inhibition to 90% of the cells, although the unpoised graphite rod also seemed toxic, inhibiting ca. 60% of Vh cells. A similar experiment with titanium (known to be non-toxic to bacteria), instead of graphite, clearly showed the vibrio-inhibiting effect of the applied redox potential. Altogether, the results directly evidence the inhibitory effect of the SBES on V. harveyi and V. parahaemolyticus. This effect, together with the mild effect on the beneficial bacilli, implies that SBES integration in aquaculture ponds can be a promising novel technological option that allow controlling vibriosis in-situ while minimizing the uses of antibiotics or other chemicals, toward more sustainable aquaculture practices. |
| first_indexed | 2024-03-08T22:46:13Z |
| format | Article |
| id | doaj.art-5b660c65829f433faa6e45dafd550ff6 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:13Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-5b660c65829f433faa6e45dafd550ff62023-12-17T06:39:52ZengElsevierAquaculture Reports2352-51342023-12-0133101826Sediment bioelectrochemical system with potential application against vibriosis in aquaculturePhuong Ha Vu0Nhung Hong Tran1Thuy Thu Thi Nguyen2Hanh My Tran3Ha Viet Thi Bui4Huy Quang Nguyen5Thao Kim Nu Nguyen6Hai The Pham7GREENLAB, Center for Life Science Research (CELIFE), Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet NamGREENLAB, Center for Life Science Research (CELIFE), Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet NamGREENLAB, Center for Life Science Research (CELIFE), Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet NamDepartment of Microbiology, Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet NamDepartment of Microbiology, Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet NamDepartment of Biochemistry and Molecular Biology, Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam; National Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, 334 Nguyen Trai, Thanh Xuan, Hanoi, VietnamDepartment of Cell Biology, Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet NamGREENLAB, Center for Life Science Research (CELIFE), Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam; Department of Microbiology, Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam; Corresponding author at: GREENLAB, Center for Life Science Research (CELIFE), Faculty of Biology, VNU University of Science, Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Viet Nam.Aquaculture can suffer from heavy losses caused by vibriosis, which denotes diseases caused by Vibrio bacteria. Due to disadvantages of available measures, mostly involving the use of antibiotics, to control these bacteria, novel measures are still needed. Therefore, aiming at an innovative solution for that issue in a long term, we investigated the inhibitory effects of the sediment bioelectrochemical system (SBES) on a V. harveyi strain (Vh) and a V. parahaemolyticus strain (Vp). SBESs were installed in test pond models that were lab-scale plastic tanks containing artificial brackish water, while the resembling systems not having SBESs were used as the controls. Once the SBES stably generated electricity (at ca. 0.5 mA), the tests with the Vibrio strains were conducted by: (i) supplementing the Vibrio cell suspensions directly into the tank waters, (ii) immersing the Vibrio cells suspensions each in an isolated axenic space into the tank environments, and (iii) adding the filtered tank waters after taken out of the tanks to the Vibrio cells. Based on plate enumeration to evaluate the inhibitory effects of such treatments on the Vibrio strains, we discovered that almost 100% of both Vh and Vp cells could not grow after 5 min in contact with the SBES water while more than 80% of them could still grow after 1–2 h in contact with the water from the control tanks. Paralleling tests showed that the SBES water was also toxic to an Escherichia coli strain but only slightly inhibited a Bacillus pumilus strain and a Lactobacillus plantarum strain, reducing their growths by only about 60% and 30%, respectively. Further tests revealed that the effects were not due to suspected proteinacous components or metal contents or peroxides in the SBES water. On the other hand, applying a redox potential of 20 mV (vs. Ag/AgCl), which was the anode potential measured in the SBES, onto a graphite rod immersed in Vh cell suspension led to the growth inhibition to 90% of the cells, although the unpoised graphite rod also seemed toxic, inhibiting ca. 60% of Vh cells. A similar experiment with titanium (known to be non-toxic to bacteria), instead of graphite, clearly showed the vibrio-inhibiting effect of the applied redox potential. Altogether, the results directly evidence the inhibitory effect of the SBES on V. harveyi and V. parahaemolyticus. This effect, together with the mild effect on the beneficial bacilli, implies that SBES integration in aquaculture ponds can be a promising novel technological option that allow controlling vibriosis in-situ while minimizing the uses of antibiotics or other chemicals, toward more sustainable aquaculture practices.http://www.sciencedirect.com/science/article/pii/S2352513423003654Aquaculture diseasesIn-situ controlVibriosisAntibiotic alternativesSediment bioelectrochemical system |
| spellingShingle | Phuong Ha Vu Nhung Hong Tran Thuy Thu Thi Nguyen Hanh My Tran Ha Viet Thi Bui Huy Quang Nguyen Thao Kim Nu Nguyen Hai The Pham Sediment bioelectrochemical system with potential application against vibriosis in aquaculture Aquaculture Reports Aquaculture diseases In-situ control Vibriosis Antibiotic alternatives Sediment bioelectrochemical system |
| title | Sediment bioelectrochemical system with potential application against vibriosis in aquaculture |
| title_full | Sediment bioelectrochemical system with potential application against vibriosis in aquaculture |
| title_fullStr | Sediment bioelectrochemical system with potential application against vibriosis in aquaculture |
| title_full_unstemmed | Sediment bioelectrochemical system with potential application against vibriosis in aquaculture |
| title_short | Sediment bioelectrochemical system with potential application against vibriosis in aquaculture |
| title_sort | sediment bioelectrochemical system with potential application against vibriosis in aquaculture |
| topic | Aquaculture diseases In-situ control Vibriosis Antibiotic alternatives Sediment bioelectrochemical system |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003654 |
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