The monitoring of biogenic amines in the raw food

The aim of this work was to evaluate microbial quality and the presence of biogenic amines in raw bars. This study was focused on microbiological research in order to determine the presence of selected indicator groups of microorganisms depending on the composition of raw food. Identification of mic...

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Main Authors: Pavel Pleva, Lucie Berčí­ková, Erika Čechová, Petr Bartošek, Leona Buňková
Format: Article
Language:English
Published: HACCP Consulting 2019-06-01
Series:Potravinarstvo
Subjects:
Online Access:https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1055
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author Pavel Pleva
Lucie Berčí­ková
Erika Čechová
Petr Bartošek
Leona Buňková
author_facet Pavel Pleva
Lucie Berčí­ková
Erika Čechová
Petr Bartošek
Leona Buňková
author_sort Pavel Pleva
collection DOAJ
description The aim of this work was to evaluate microbial quality and the presence of biogenic amines in raw bars. This study was focused on microbiological research in order to determine the presence of selected indicator groups of microorganisms depending on the composition of raw food. Identification of microorganisms was carried out by MALDI-TOF MS. In the second part of the experiment, biogenic amines and polyamines were analyzed using high performance liquid chromatography with UV/VIS detection. An increased incidence of mold has been reported in the samples, which is associated with a risk of mycotoxin production. After identifying microorganisms, it was found out that genera Micrococcus, Bacillus and Staphylococcus were the most represented. The highest concentration of biogenic amines (tyramine 42.2 ±4.8 mg.kg-1; putrescine 54.0 ±2.9 mg.kg-1) was found in a sample containing the vegetable component. The average concentration of biogenic amines in the tested raw bars was <30 mg.kg-1 and therefore they do not pose a serious health hazard to a consumer.
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spelling doaj.art-5b66c77bda9848e3a3e6f3311cc4239e2022-12-21T21:17:20ZengHACCP ConsultingPotravinarstvo1337-09602019-06-0113148248910.5219/1055779The monitoring of biogenic amines in the raw foodPavel Pleva0Lucie Berčí­ková1Erika Čechová2https://orcid.org/0000-0003-1141-6530Petr Bartošek3Leona Buňková4Tomas Bata University in Zlí­n, Faculty of Technology, Department of Environmental Protection Engineering, nám. T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic, Tel.: +420576031209Tomas Bata University in Zlí­n, Faculty of Technology, Environmental Protection Engineering, T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic, Tel.: 00420 576 031Tomas Bata University in Zlí­n, Faculty of Technology, Environmental Protection Engineering, T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic, Tel.: 00420 576 031 209 Tomas Bata University in Zlí­n, Faculty of Technology, Department of Food Technology, T. G. Masaryka 5555, 760 01 Zlí­n, Czech Republic, Tel.: 00420 576 031 209 Tomas Bata University in Zlí­n, Faculty of Technology, Environmental Protection Engineering, T. G. Masaryka 5555, 760 01 Zlí­n, Czech RepublicThe aim of this work was to evaluate microbial quality and the presence of biogenic amines in raw bars. This study was focused on microbiological research in order to determine the presence of selected indicator groups of microorganisms depending on the composition of raw food. Identification of microorganisms was carried out by MALDI-TOF MS. In the second part of the experiment, biogenic amines and polyamines were analyzed using high performance liquid chromatography with UV/VIS detection. An increased incidence of mold has been reported in the samples, which is associated with a risk of mycotoxin production. After identifying microorganisms, it was found out that genera Micrococcus, Bacillus and Staphylococcus were the most represented. The highest concentration of biogenic amines (tyramine 42.2 ±4.8 mg.kg-1; putrescine 54.0 ±2.9 mg.kg-1) was found in a sample containing the vegetable component. The average concentration of biogenic amines in the tested raw bars was <30 mg.kg-1 and therefore they do not pose a serious health hazard to a consumer.https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1055raw foodbiogenic aminesuhplcmicroorganisms
spellingShingle Pavel Pleva
Lucie Berčí­ková
Erika Čechová
Petr Bartošek
Leona Buňková
The monitoring of biogenic amines in the raw food
Potravinarstvo
raw food
biogenic amines
uhplc
microorganisms
title The monitoring of biogenic amines in the raw food
title_full The monitoring of biogenic amines in the raw food
title_fullStr The monitoring of biogenic amines in the raw food
title_full_unstemmed The monitoring of biogenic amines in the raw food
title_short The monitoring of biogenic amines in the raw food
title_sort monitoring of biogenic amines in the raw food
topic raw food
biogenic amines
uhplc
microorganisms
url https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1055
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