Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma Multiforme
In this study, we demonstrate that Raman microscopy combined with computational analysis is a useful approach to discriminating accurately between brain tumor bio-specimens and to identifying structural changes in glioblastoma (GBM) bio-signatures after nordihydroguaiaretic acid (NDGA) administratio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/7/2643 |
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| author | Felicia S. Manciu Jose Guerrero Kevin E. Bennet Su-Youne Chang Masum Rahman Lizbeth V. Martinez Lopez Siobhan Chantigian Mariana Castellanos Marian Manciu |
| author_facet | Felicia S. Manciu Jose Guerrero Kevin E. Bennet Su-Youne Chang Masum Rahman Lizbeth V. Martinez Lopez Siobhan Chantigian Mariana Castellanos Marian Manciu |
| author_sort | Felicia S. Manciu |
| collection | DOAJ |
| description | In this study, we demonstrate that Raman microscopy combined with computational analysis is a useful approach to discriminating accurately between brain tumor bio-specimens and to identifying structural changes in glioblastoma (GBM) bio-signatures after nordihydroguaiaretic acid (NDGA) administration. NDGA phenolic lignan was selected as a potential therapeutic agent because of its reported beneficial effects in alleviating and inhibiting the formation of multi-organ malignant tumors. The current analysis of NDGA’s impact on GBM human cells demonstrates a reduction in the quantity of altered protein content and of reactive oxygen species (ROS)-damaged phenylalanine; results that correlate with the ROS scavenger and anti-oxidant properties of NDGA. A novel outcome presented here is the use of phenylalanine as a biomarker for differentiating between samples and assessing drug efficacy. Treatment with a low NDGA dose shows a decline in abnormal lipid-protein metabolism, which is inferred by the formation of lipid droplets and a decrease in altered protein content. A very high dose results in cell structural and membrane damage that favors transformed protein overexpression. The information gained through this work is of substantial value for understanding NDGA’s beneficial as well as detrimental bio-effects as a potential therapeutic drug for brain cancer. |
| first_indexed | 2024-03-09T11:25:15Z |
| format | Article |
| id | doaj.art-5b68cc0ca1124144a5a7feba94a66722 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T11:25:15Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-5b68cc0ca1124144a5a7feba94a667222023-12-01T00:02:39ZengMDPI AGSensors1424-82202022-03-01227264310.3390/s22072643Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma MultiformeFelicia S. Manciu0Jose Guerrero1Kevin E. Bennet2Su-Youne Chang3Masum Rahman4Lizbeth V. Martinez Lopez5Siobhan Chantigian6Mariana Castellanos7Marian Manciu8Department of Physics, University of Texas at El Paso, El Paso, TX 79968, USADepartment of Physics, University of Texas at El Paso, El Paso, TX 79968, USADepartment of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USADepartment of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USADepartment of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USADepartment of Physics, University of Texas at El Paso, El Paso, TX 79968, USADepartment of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USADepartment of Physics, University of Texas at El Paso, El Paso, TX 79968, USADepartment of Physics, University of Texas at El Paso, El Paso, TX 79968, USAIn this study, we demonstrate that Raman microscopy combined with computational analysis is a useful approach to discriminating accurately between brain tumor bio-specimens and to identifying structural changes in glioblastoma (GBM) bio-signatures after nordihydroguaiaretic acid (NDGA) administration. NDGA phenolic lignan was selected as a potential therapeutic agent because of its reported beneficial effects in alleviating and inhibiting the formation of multi-organ malignant tumors. The current analysis of NDGA’s impact on GBM human cells demonstrates a reduction in the quantity of altered protein content and of reactive oxygen species (ROS)-damaged phenylalanine; results that correlate with the ROS scavenger and anti-oxidant properties of NDGA. A novel outcome presented here is the use of phenylalanine as a biomarker for differentiating between samples and assessing drug efficacy. Treatment with a low NDGA dose shows a decline in abnormal lipid-protein metabolism, which is inferred by the formation of lipid droplets and a decrease in altered protein content. A very high dose results in cell structural and membrane damage that favors transformed protein overexpression. The information gained through this work is of substantial value for understanding NDGA’s beneficial as well as detrimental bio-effects as a potential therapeutic drug for brain cancer.https://www.mdpi.com/1424-8220/22/7/2643Raman microscopyopticalstatistical analysisprincipal component analysisglioblastoma cellsnordihydroguaiaretic acid |
| spellingShingle | Felicia S. Manciu Jose Guerrero Kevin E. Bennet Su-Youne Chang Masum Rahman Lizbeth V. Martinez Lopez Siobhan Chantigian Mariana Castellanos Marian Manciu Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma Multiforme Sensors Raman microscopy optical statistical analysis principal component analysis glioblastoma cells nordihydroguaiaretic acid |
| title | Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma Multiforme |
| title_full | Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma Multiforme |
| title_fullStr | Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma Multiforme |
| title_full_unstemmed | Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma Multiforme |
| title_short | Assessing Nordihydroguaiaretic Acid Therapeutic Effect for Glioblastoma Multiforme |
| title_sort | assessing nordihydroguaiaretic acid therapeutic effect for glioblastoma multiforme |
| topic | Raman microscopy optical statistical analysis principal component analysis glioblastoma cells nordihydroguaiaretic acid |
| url | https://www.mdpi.com/1424-8220/22/7/2643 |
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