Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing Agent
The biocidal properties of silver nanoparticles (AgNPs) prepared with the use of biologically active compounds seem to be especially significant for biological and medical application. Therefore, the aim of this research was to determine and compare the antibacterial and fungicidal properties of fif...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/10/1481 |
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| author | Agnieszka Gibała Paulina Żeliszewska Tomasz Gosiewski Agnieszka Krawczyk Dorota Duraczyńska Joanna Szaleniec Maciej Szaleniec Magdalena Oćwieja |
| author_facet | Agnieszka Gibała Paulina Żeliszewska Tomasz Gosiewski Agnieszka Krawczyk Dorota Duraczyńska Joanna Szaleniec Maciej Szaleniec Magdalena Oćwieja |
| author_sort | Agnieszka Gibała |
| collection | DOAJ |
| description | The biocidal properties of silver nanoparticles (AgNPs) prepared with the use of biologically active compounds seem to be especially significant for biological and medical application. Therefore, the aim of this research was to determine and compare the antibacterial and fungicidal properties of fifteen types of AgNPs. The main hypothesis was that the biological activity of AgNPs characterized by comparable size distributions, shapes, and ion release profiles is dependent on the properties of stabilizing agent molecules adsorbed on their surfaces. <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> were selected as models of two types of bacterial cells. <i>Candida albicans</i> was selected for the research as a representative type of eukaryotic microorganism. The conducted studies reveal that larger AgNPs can be more biocidal than smaller ones. It was found that positively charged arginine-stabilized AgNPs (ARGSBAgNPs) were the most biocidal among all studied nanoparticles. The strongest fungicidal properties were detected for negatively charged EGCGAgNPs obtained using (−)-epigallocatechin gallate (EGCG). It was concluded that, by applying a specific stabilizing agent, one can tune the selectivity of AgNP toxicity towards desired pathogens. It was established that <i>E. coli</i> was more sensitive to AgNP exposure than <i>S. aureus</i> regardless of AgNP size and surface properties. |
| first_indexed | 2024-03-10T06:42:23Z |
| format | Article |
| id | doaj.art-5b6a6ee508994fb99d3e009d2261a20b |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T06:42:23Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-5b6a6ee508994fb99d3e009d2261a20b2023-11-22T17:34:15ZengMDPI AGBiomolecules2218-273X2021-10-011110148110.3390/biom11101481Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing AgentAgnieszka Gibała0Paulina Żeliszewska1Tomasz Gosiewski2Agnieszka Krawczyk3Dorota Duraczyńska4Joanna Szaleniec5Maciej Szaleniec6Magdalena Oćwieja7Department of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Czysta 18, 31-12 Krakow, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandDepartment of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Czysta 18, 31-12 Krakow, PolandDepartment of Molecular Medical Microbiology, Chair of Microbiology, Faculty of Medicine, Jagiellonian University Medical College, Czysta 18, 31-12 Krakow, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandDepartment of Otolaryngology, Faculty of Medicine, Jagiellonian University Medical College, Jakubowskiego 2, 30-688 Krakow, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandJerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, PolandThe biocidal properties of silver nanoparticles (AgNPs) prepared with the use of biologically active compounds seem to be especially significant for biological and medical application. Therefore, the aim of this research was to determine and compare the antibacterial and fungicidal properties of fifteen types of AgNPs. The main hypothesis was that the biological activity of AgNPs characterized by comparable size distributions, shapes, and ion release profiles is dependent on the properties of stabilizing agent molecules adsorbed on their surfaces. <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> were selected as models of two types of bacterial cells. <i>Candida albicans</i> was selected for the research as a representative type of eukaryotic microorganism. The conducted studies reveal that larger AgNPs can be more biocidal than smaller ones. It was found that positively charged arginine-stabilized AgNPs (ARGSBAgNPs) were the most biocidal among all studied nanoparticles. The strongest fungicidal properties were detected for negatively charged EGCGAgNPs obtained using (−)-epigallocatechin gallate (EGCG). It was concluded that, by applying a specific stabilizing agent, one can tune the selectivity of AgNP toxicity towards desired pathogens. It was established that <i>E. coli</i> was more sensitive to AgNP exposure than <i>S. aureus</i> regardless of AgNP size and surface properties.https://www.mdpi.com/2218-273X/11/10/1481silver nanoparticlesbiocidal propertiessurface propertiesGram-negative bacteriaGram-positive bacteriapathogenic fungi |
| spellingShingle | Agnieszka Gibała Paulina Żeliszewska Tomasz Gosiewski Agnieszka Krawczyk Dorota Duraczyńska Joanna Szaleniec Maciej Szaleniec Magdalena Oćwieja Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing Agent Biomolecules silver nanoparticles biocidal properties surface properties Gram-negative bacteria Gram-positive bacteria pathogenic fungi |
| title | Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing Agent |
| title_full | Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing Agent |
| title_fullStr | Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing Agent |
| title_full_unstemmed | Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing Agent |
| title_short | Antibacterial and Antifungal Properties of Silver Nanoparticles—Effect of a Surface-Stabilizing Agent |
| title_sort | antibacterial and antifungal properties of silver nanoparticles effect of a surface stabilizing agent |
| topic | silver nanoparticles biocidal properties surface properties Gram-negative bacteria Gram-positive bacteria pathogenic fungi |
| url | https://www.mdpi.com/2218-273X/11/10/1481 |
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