Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana
Chloroplast thylakoid protein rubredoxin 1 (RBD1) in Chlamydomonas and its cyanobacterial homolog RubA contain a rubredoxin domain. These proteins have been proposed to participate in the assembly of photosystem II (PSII) at early stages. However, the effects of inactivation of RBD1 on PSII assembly...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.824358/full |
| _version_ | 1818329817965133824 |
|---|---|
| author | Liping Che Han Meng Junxiang Ruan Lianwei Peng Lin Zhang |
| author_facet | Liping Che Han Meng Junxiang Ruan Lianwei Peng Lin Zhang |
| author_sort | Liping Che |
| collection | DOAJ |
| description | Chloroplast thylakoid protein rubredoxin 1 (RBD1) in Chlamydomonas and its cyanobacterial homolog RubA contain a rubredoxin domain. These proteins have been proposed to participate in the assembly of photosystem II (PSII) at early stages. However, the effects of inactivation of RBD1 on PSII assembly in higher plants are largely unclear. Here, we characterized an Arabidopsis rbd1 mutant in detail. A drastic reduction of intact PSII complex but relatively higher levels of assembly intermediates including PSII RC, pre-CP47, and pre-CP43 were found in rbd1. Polysome association and ribosome profiling revealed that ribosome recruitment of psbA mRNA is specifically reduced. Consistently, in vivo protein pulse-chase labeling showed that the rate of D1/pD1 synthesis is significantly reduced in rbd1 compared with WT. Moreover, newly synthesized mature D1 and pD1/D2 can assemble into the PSII reaction center (RC) complex but further formation of larger PSII complexes is nearly totally blocked in rbd1. Our data imply that RBD1 is not only required for the formation of a functional PSII core complex during the early stages of PSII assembly but may also be involved in the translation of D1 in higher plants. |
| first_indexed | 2024-12-13T12:54:06Z |
| format | Article |
| id | doaj.art-5b6af6b977a14410b6475facc9ac44b3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T12:54:06Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5b6af6b977a14410b6475facc9ac44b32022-12-21T23:45:14ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-02-011310.3389/fpls.2022.824358824358Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thalianaLiping Che0Han Meng1Junxiang Ruan2Lianwei Peng3Lin Zhang4School of Environmental and Geographical Sciences, Shanghai Normal University, Shanghai, ChinaShanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, ChinaSchool of Environmental and Geographical Sciences, Shanghai Normal University, Shanghai, ChinaShanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, ChinaShanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, ChinaChloroplast thylakoid protein rubredoxin 1 (RBD1) in Chlamydomonas and its cyanobacterial homolog RubA contain a rubredoxin domain. These proteins have been proposed to participate in the assembly of photosystem II (PSII) at early stages. However, the effects of inactivation of RBD1 on PSII assembly in higher plants are largely unclear. Here, we characterized an Arabidopsis rbd1 mutant in detail. A drastic reduction of intact PSII complex but relatively higher levels of assembly intermediates including PSII RC, pre-CP47, and pre-CP43 were found in rbd1. Polysome association and ribosome profiling revealed that ribosome recruitment of psbA mRNA is specifically reduced. Consistently, in vivo protein pulse-chase labeling showed that the rate of D1/pD1 synthesis is significantly reduced in rbd1 compared with WT. Moreover, newly synthesized mature D1 and pD1/D2 can assemble into the PSII reaction center (RC) complex but further formation of larger PSII complexes is nearly totally blocked in rbd1. Our data imply that RBD1 is not only required for the formation of a functional PSII core complex during the early stages of PSII assembly but may also be involved in the translation of D1 in higher plants.https://www.frontiersin.org/articles/10.3389/fpls.2022.824358/fullrubredoxinphotosystem IIreaction centerpsbAribosome profilingArabidopsis thaliana |
| spellingShingle | Liping Che Han Meng Junxiang Ruan Lianwei Peng Lin Zhang Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana Frontiers in Plant Science rubredoxin photosystem II reaction center psbA ribosome profiling Arabidopsis thaliana |
| title | Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana |
| title_full | Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana |
| title_fullStr | Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana |
| title_full_unstemmed | Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana |
| title_short | Rubredoxin 1 Is Required for Formation of the Functional Photosystem II Core Complex in Arabidopsis thaliana |
| title_sort | rubredoxin 1 is required for formation of the functional photosystem ii core complex in arabidopsis thaliana |
| topic | rubredoxin photosystem II reaction center psbA ribosome profiling Arabidopsis thaliana |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.824358/full |
| work_keys_str_mv | AT lipingche rubredoxin1isrequiredforformationofthefunctionalphotosystemiicorecomplexinarabidopsisthaliana AT hanmeng rubredoxin1isrequiredforformationofthefunctionalphotosystemiicorecomplexinarabidopsisthaliana AT junxiangruan rubredoxin1isrequiredforformationofthefunctionalphotosystemiicorecomplexinarabidopsisthaliana AT lianweipeng rubredoxin1isrequiredforformationofthefunctionalphotosystemiicorecomplexinarabidopsisthaliana AT linzhang rubredoxin1isrequiredforformationofthefunctionalphotosystemiicorecomplexinarabidopsisthaliana |