Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases
Newly found biochemical characteristics of the placenta can provide new insights for further studies on the possible markers of physiological/pathological pregnancy or the function of the placenta. We compared the proteome of the dairy cow placenta after enzymatic hydrolysis by three different prote...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/21/3395 |
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| author | Liuhong Shen Zeru Zhang Yue Zhang Yuquan Zhao Lei Fan Shumin Yu Suizhong Cao Yixin Huang |
| author_facet | Liuhong Shen Zeru Zhang Yue Zhang Yuquan Zhao Lei Fan Shumin Yu Suizhong Cao Yixin Huang |
| author_sort | Liuhong Shen |
| collection | DOAJ |
| description | Newly found biochemical characteristics of the placenta can provide new insights for further studies on the possible markers of physiological/pathological pregnancy or the function of the placenta. We compared the proteome of the dairy cow placenta after enzymatic hydrolysis by three different proteases using a label-free mass spectrometry approach. In total, 541, 136, and 86 proteins were identified in the trypsin group (TRY), pepsin group (PEP), and papain group (PAP). By comparing the proteome of the PAP and TRY, PEP and TRY, and PEP and PAP groups, 432, 421, and 136 differentially expressed proteins were identified, respectively. We compared the up-regulated DEPs and down-regulated DEPs of each comparison group. The results show that the proteins identified by papain were mostly derived from the extracellular matrix and collagen, and were enriched in the relaxin signaling pathway and AGE-RAGE signaling pathway in diabetic complications; pepsin digestion was able to identify more muscle-related proteins, which were enriched in the lysosome, platelet activation, cardiac muscle contraction, the bacterial invasion of epithelial cells, and small cell lung cancer; trypsin mainly enzymatically degraded the extracellular matrix, blood particles, and cell-surface proteins that were enriched in arginine and proline metabolism, olfactory transduction proteasome, protein processing in the endoplasmic reticulum, pyruvate metabolism, and arrhythmogenic right ventricular cardiomyopathy (ARVC). In summary, these results provide insights into the discovery of the physiological functions of dairy cow placenta and the selection of proteases in dairy cow placenta proteomics. |
| first_indexed | 2024-03-11T11:34:36Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T11:34:36Z |
| publishDate | 2023-11-01 |
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| series | Animals |
| spelling | doaj.art-5b6bafcaa2c143dd8100f6403157113e2023-11-10T14:57:58ZengMDPI AGAnimals2076-26152023-11-011321339510.3390/ani13213395Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different ProteasesLiuhong Shen0Zeru Zhang1Yue Zhang2Yuquan Zhao3Lei Fan4Shumin Yu5Suizhong Cao6Yixin Huang7The Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaThe Key Laboratory of Animal Disease and Human Health of Sichuan Province, The Medical Research Center for Cow Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaNewly found biochemical characteristics of the placenta can provide new insights for further studies on the possible markers of physiological/pathological pregnancy or the function of the placenta. We compared the proteome of the dairy cow placenta after enzymatic hydrolysis by three different proteases using a label-free mass spectrometry approach. In total, 541, 136, and 86 proteins were identified in the trypsin group (TRY), pepsin group (PEP), and papain group (PAP). By comparing the proteome of the PAP and TRY, PEP and TRY, and PEP and PAP groups, 432, 421, and 136 differentially expressed proteins were identified, respectively. We compared the up-regulated DEPs and down-regulated DEPs of each comparison group. The results show that the proteins identified by papain were mostly derived from the extracellular matrix and collagen, and were enriched in the relaxin signaling pathway and AGE-RAGE signaling pathway in diabetic complications; pepsin digestion was able to identify more muscle-related proteins, which were enriched in the lysosome, platelet activation, cardiac muscle contraction, the bacterial invasion of epithelial cells, and small cell lung cancer; trypsin mainly enzymatically degraded the extracellular matrix, blood particles, and cell-surface proteins that were enriched in arginine and proline metabolism, olfactory transduction proteasome, protein processing in the endoplasmic reticulum, pyruvate metabolism, and arrhythmogenic right ventricular cardiomyopathy (ARVC). In summary, these results provide insights into the discovery of the physiological functions of dairy cow placenta and the selection of proteases in dairy cow placenta proteomics.https://www.mdpi.com/2076-2615/13/21/3395dairy cowsplacentatrypsinpapainpepsinlabel-free |
| spellingShingle | Liuhong Shen Zeru Zhang Yue Zhang Yuquan Zhao Lei Fan Shumin Yu Suizhong Cao Yixin Huang Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases Animals dairy cows placenta trypsin papain pepsin label-free |
| title | Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases |
| title_full | Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases |
| title_fullStr | Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases |
| title_full_unstemmed | Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases |
| title_short | Analysis and Comparison of Proteomics of Placental Proteins from Cows Using Different Proteases |
| title_sort | analysis and comparison of proteomics of placental proteins from cows using different proteases |
| topic | dairy cows placenta trypsin papain pepsin label-free |
| url | https://www.mdpi.com/2076-2615/13/21/3395 |
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