Neutrophil extracellular traps induce the bone erosion of gout
Abstract Objective To investigate the relationships between monosodium urate (MSU) crystals -induced neutrophil extracellular traps (NETs) and bone erosion in gout. Methods Animal models were used to study the relationship between NETs induced by MSU crystals and bone erosion. Neutrophils were treat...
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12891-022-06115-w |
| _version_ | 1797973774590541824 |
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| author | Ertao Jia Zhiling Li Hongling Geng Haiqiong Zhu Yadong Wang Feng Lin Yubao Jiang Jianyong Zhang |
| author_facet | Ertao Jia Zhiling Li Hongling Geng Haiqiong Zhu Yadong Wang Feng Lin Yubao Jiang Jianyong Zhang |
| author_sort | Ertao Jia |
| collection | DOAJ |
| description | Abstract Objective To investigate the relationships between monosodium urate (MSU) crystals -induced neutrophil extracellular traps (NETs) and bone erosion in gout. Methods Animal models were used to study the relationship between NETs induced by MSU crystals and bone erosion. Neutrophils were treated with MSU crystals to induce NETs. The osteoblasts-like cells (OB) were then treated with NETs, and the supernatant was co-incubated with osteoclasts-like cells (OC). The NETs were digested with DNase, and the neutrophil elastase (NE) was inhibited with sivelestat sodium. Cell viability, mRNA, and protein expression were also assessed. Results After treating OB with NETs, the cell viability decreased. Yet, after digesting the DNA and inhibiting NE, the viability was moderately improved. The expression level of osteoprotegerin (OPG) and alkaline phosphatase (ALP) was up-regulated, while the expression level of receptor activator of nuclear factor kappa-B ligand (RANKL) was down-regulated in the sivelestat sodium + MSU group compared with MSU group. The number of OC was significantly elevated. In contrast, the number of OB was not increased in the tibia after establishing the gout model. The supernatant obtained from OB was treated with NETs promoting OC differentiation. The expression level of receptor activator of nuclear factor kappa-B (RANK), tartrate-resistant acid phosphatase (TRAP), and cathepsin K (Cst K) was up-regulated in the MSU group compared with the normal control (NC) group. Conclusion NETs induced by MSU crystals could inhibit osteoblasts viability and enhance the activity of osteoclasts. |
| first_indexed | 2024-04-11T04:09:33Z |
| format | Article |
| id | doaj.art-5b6d34e6db3d49269c2e687bd65c4187 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-04-11T04:09:33Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-5b6d34e6db3d49269c2e687bd65c41872023-01-01T12:11:08ZengBMCBMC Musculoskeletal Disorders1471-24742022-12-012311910.1186/s12891-022-06115-wNeutrophil extracellular traps induce the bone erosion of goutErtao Jia0Zhiling Li1Hongling Geng2Haiqiong Zhu3Yadong Wang4Feng Lin5Yubao Jiang6Jianyong Zhang7The Department of Rheumatology, the Fourth Clinical Medical College of Guangzhou University of Chinese MedicineThe Fourth Clinical Medical College of Guangzhou University of Chinese MedicineThe Department of Gynecology, Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese MedicineShenzhen Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese MedicineThe Department of Urology, Shenzhen Traditional Chinese Medicine HospitalThe Department of Urology, Shenzhen Traditional Chinese Medicine HospitalThe Department of Rheumatology, the Fourth Clinical Medical College of Guangzhou University of Chinese MedicineThe Department of Rheumatology, the Fourth Clinical Medical College of Guangzhou University of Chinese MedicineAbstract Objective To investigate the relationships between monosodium urate (MSU) crystals -induced neutrophil extracellular traps (NETs) and bone erosion in gout. Methods Animal models were used to study the relationship between NETs induced by MSU crystals and bone erosion. Neutrophils were treated with MSU crystals to induce NETs. The osteoblasts-like cells (OB) were then treated with NETs, and the supernatant was co-incubated with osteoclasts-like cells (OC). The NETs were digested with DNase, and the neutrophil elastase (NE) was inhibited with sivelestat sodium. Cell viability, mRNA, and protein expression were also assessed. Results After treating OB with NETs, the cell viability decreased. Yet, after digesting the DNA and inhibiting NE, the viability was moderately improved. The expression level of osteoprotegerin (OPG) and alkaline phosphatase (ALP) was up-regulated, while the expression level of receptor activator of nuclear factor kappa-B ligand (RANKL) was down-regulated in the sivelestat sodium + MSU group compared with MSU group. The number of OC was significantly elevated. In contrast, the number of OB was not increased in the tibia after establishing the gout model. The supernatant obtained from OB was treated with NETs promoting OC differentiation. The expression level of receptor activator of nuclear factor kappa-B (RANK), tartrate-resistant acid phosphatase (TRAP), and cathepsin K (Cst K) was up-regulated in the MSU group compared with the normal control (NC) group. Conclusion NETs induced by MSU crystals could inhibit osteoblasts viability and enhance the activity of osteoclasts.https://doi.org/10.1186/s12891-022-06115-wGoutNeutrophil extracellular trapsOsteoblastsOsteoclasts |
| spellingShingle | Ertao Jia Zhiling Li Hongling Geng Haiqiong Zhu Yadong Wang Feng Lin Yubao Jiang Jianyong Zhang Neutrophil extracellular traps induce the bone erosion of gout BMC Musculoskeletal Disorders Gout Neutrophil extracellular traps Osteoblasts Osteoclasts |
| title | Neutrophil extracellular traps induce the bone erosion of gout |
| title_full | Neutrophil extracellular traps induce the bone erosion of gout |
| title_fullStr | Neutrophil extracellular traps induce the bone erosion of gout |
| title_full_unstemmed | Neutrophil extracellular traps induce the bone erosion of gout |
| title_short | Neutrophil extracellular traps induce the bone erosion of gout |
| title_sort | neutrophil extracellular traps induce the bone erosion of gout |
| topic | Gout Neutrophil extracellular traps Osteoblasts Osteoclasts |
| url | https://doi.org/10.1186/s12891-022-06115-w |
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