Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria
This multi-center study evaluated a novel microscope system capable of quantitative phase microscopy (QPM) for label-free sperm-cell selection for intracytoplasmic sperm injection (ICSI). Seventy-three patients were enrolled in four in vitro fertilization (IVF) units, where senior embryologists were...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/10/2614 |
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| author | Yulia Michailov Luba Nemerovsky Yehudith Ghetler Maya Finkelstein Oshrat Schonberger Amir Wiser Arie Raziel Bozhena Saar-Ryss Ido Ben-Ami Olga Kaplanski Netanella Miller Einat Haikin Herzberger Yardena Mashiach Friedler Tali Levitas-Djerbi Eden Amsalem Natalia Umanski Valeria Tamadaev Yaniv S. Ovadia Aharon Peretz Gilat Sacks Nava Dekel Odelya Zaken Mattan Levi |
| author_facet | Yulia Michailov Luba Nemerovsky Yehudith Ghetler Maya Finkelstein Oshrat Schonberger Amir Wiser Arie Raziel Bozhena Saar-Ryss Ido Ben-Ami Olga Kaplanski Netanella Miller Einat Haikin Herzberger Yardena Mashiach Friedler Tali Levitas-Djerbi Eden Amsalem Natalia Umanski Valeria Tamadaev Yaniv S. Ovadia Aharon Peretz Gilat Sacks Nava Dekel Odelya Zaken Mattan Levi |
| author_sort | Yulia Michailov |
| collection | DOAJ |
| description | This multi-center study evaluated a novel microscope system capable of quantitative phase microscopy (QPM) for label-free sperm-cell selection for intracytoplasmic sperm injection (ICSI). Seventy-three patients were enrolled in four in vitro fertilization (IVF) units, where senior embryologists were asked to select 11 apparently normal and 11 overtly abnormal sperm cells, in accordance with current clinical practice, using a micromanipulator and 60× bright field microscopy. Following sperm selection and imaging via QPM, the individual sperm cell was chemically stained per World Health Organization (WHO) 2021 protocols and imaged via bright field microscopy for subsequent manual measurements by embryologists who were blinded to the QPM measurements. A comparison of the two modalities resulted in mean differences of 0.18 µm (CI −0.442–0.808 µm, 95%, STD—0.32 µm) for head length, −0.26 µm (CI −0.86–0.33 µm, 95%, STD—0.29 µm) for head width, 0.17 (CI −0.12–0.478, 95%, STD—0.15) for length–width ratio and 5.7 for acrosome–head area ratio (CI −12.81–24.33, 95%, STD—9.6). The repeatability of the measurements was significantly higher in the QPM modality. Surprisingly, only 19% of the subjectively pre-selected normal cells were found to be normal according to the WHO2021 criteria. The measurements of cells imaged stain-free through QPM were found to be in good agreement with the measurements performed on the reference method of stained cells imaged through bright field microscopy. QPM is non-toxic and non-invasive and can improve the clinical effectiveness of ICSI by choosing sperm cells that meet the strict criteria of the WHO2021. |
| first_indexed | 2024-03-10T21:25:23Z |
| format | Article |
| id | doaj.art-5b6ff7a89dbd43f7be88fdee5c653836 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T21:25:23Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-5b6ff7a89dbd43f7be88fdee5c6538362023-11-19T15:44:33ZengMDPI AGBiomedicines2227-90592023-09-011110261410.3390/biomedicines11102614Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal CriteriaYulia Michailov0Luba Nemerovsky1Yehudith Ghetler2Maya Finkelstein3Oshrat Schonberger4Amir Wiser5Arie Raziel6Bozhena Saar-Ryss7Ido Ben-Ami8Olga Kaplanski9Netanella Miller10Einat Haikin Herzberger11Yardena Mashiach Friedler12Tali Levitas-Djerbi13Eden Amsalem14Natalia Umanski15Valeria Tamadaev16Yaniv S. Ovadia17Aharon Peretz18Gilat Sacks19Nava Dekel20Odelya Zaken21Mattan Levi22Obstetrics and Gynecology Department, Barzilai University Medical Center, Ashkelon 7830604, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelIVF Unit, Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba 4428163, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelIVF Unit, Shaare Zedek Medical Center, Jerusalem 9103102, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelObstetrics and Gynecology Department, Barzilai University Medical Center, Ashkelon 7830604, IsraelIVF Unit, Shaare Zedek Medical Center, Jerusalem 9103102, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelObstetrics and Gynecology Department, Barzilai University Medical Center, Ashkelon 7830604, IsraelObstetrics and Gynecology Department, Barzilai University Medical Center, Ashkelon 7830604, IsraelObstetrics and Gynecology Department, Barzilai University Medical Center, Ashkelon 7830604, IsraelObstetrics and Gynecology Department, Barzilai University Medical Center, Ashkelon 7830604, IsraelIVF Unit, Shaare Zedek Medical Center, Jerusalem 9103102, IsraelIVF Unit, Shaare Zedek Medical Center, Jerusalem 9103102, IsraelIVF Unit, Shaare Zedek Medical Center, Jerusalem 9103102, IsraelIVF Unit, Shaare Zedek Medical Center, Jerusalem 9103102, IsraelFaculty of Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelThis multi-center study evaluated a novel microscope system capable of quantitative phase microscopy (QPM) for label-free sperm-cell selection for intracytoplasmic sperm injection (ICSI). Seventy-three patients were enrolled in four in vitro fertilization (IVF) units, where senior embryologists were asked to select 11 apparently normal and 11 overtly abnormal sperm cells, in accordance with current clinical practice, using a micromanipulator and 60× bright field microscopy. Following sperm selection and imaging via QPM, the individual sperm cell was chemically stained per World Health Organization (WHO) 2021 protocols and imaged via bright field microscopy for subsequent manual measurements by embryologists who were blinded to the QPM measurements. A comparison of the two modalities resulted in mean differences of 0.18 µm (CI −0.442–0.808 µm, 95%, STD—0.32 µm) for head length, −0.26 µm (CI −0.86–0.33 µm, 95%, STD—0.29 µm) for head width, 0.17 (CI −0.12–0.478, 95%, STD—0.15) for length–width ratio and 5.7 for acrosome–head area ratio (CI −12.81–24.33, 95%, STD—9.6). The repeatability of the measurements was significantly higher in the QPM modality. Surprisingly, only 19% of the subjectively pre-selected normal cells were found to be normal according to the WHO2021 criteria. The measurements of cells imaged stain-free through QPM were found to be in good agreement with the measurements performed on the reference method of stained cells imaged through bright field microscopy. QPM is non-toxic and non-invasive and can improve the clinical effectiveness of ICSI by choosing sperm cells that meet the strict criteria of the WHO2021.https://www.mdpi.com/2227-9059/11/10/2614sperm selectionIVF ICSIquantitative phase microscopy (QPM)WHO2021 |
| spellingShingle | Yulia Michailov Luba Nemerovsky Yehudith Ghetler Maya Finkelstein Oshrat Schonberger Amir Wiser Arie Raziel Bozhena Saar-Ryss Ido Ben-Ami Olga Kaplanski Netanella Miller Einat Haikin Herzberger Yardena Mashiach Friedler Tali Levitas-Djerbi Eden Amsalem Natalia Umanski Valeria Tamadaev Yaniv S. Ovadia Aharon Peretz Gilat Sacks Nava Dekel Odelya Zaken Mattan Levi Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria Biomedicines sperm selection IVF ICSI quantitative phase microscopy (QPM) WHO2021 |
| title | Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria |
| title_full | Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria |
| title_fullStr | Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria |
| title_full_unstemmed | Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria |
| title_short | Stain-Free Sperm Analysis and Selection for Intracytoplasmic Sperm Injection Complying with WHO Strict Normal Criteria |
| title_sort | stain free sperm analysis and selection for intracytoplasmic sperm injection complying with who strict normal criteria |
| topic | sperm selection IVF ICSI quantitative phase microscopy (QPM) WHO2021 |
| url | https://www.mdpi.com/2227-9059/11/10/2614 |
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