Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth.
Explants of embryonic chick sympathetic and sensory ganglia were found to exhibit asymmetric radial outgrowth of neurites under standard culture conditions with or without exogenous Nerve Growth Factor [NGF]. Opposing sides of an explant exhibited: a) differences in neurite length and, b) difference...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0216263 |
| _version_ | 1819111955444531200 |
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| author | David B Pettigrew Curtis B Dobson Lori G Isaacson Eric C Leuthardt Heather N Lilley Georgette L Suidan Keith A Crutcher |
| author_facet | David B Pettigrew Curtis B Dobson Lori G Isaacson Eric C Leuthardt Heather N Lilley Georgette L Suidan Keith A Crutcher |
| author_sort | David B Pettigrew |
| collection | DOAJ |
| description | Explants of embryonic chick sympathetic and sensory ganglia were found to exhibit asymmetric radial outgrowth of neurites under standard culture conditions with or without exogenous Nerve Growth Factor [NGF]. Opposing sides of an explant exhibited: a) differences in neurite length and, b) differences in neurite morphology. Strikingly, this asymmetry exhibited co-orientation among segregated, neighboring explants. The underlying mechanism(s) of the asymmetry and its co-orientation are not known but appear to depend on cell clustering because dissociated sympathetic neurons do not exhibit co-orientation whereas re-aggregated clusters of cells do. This emergent behavior may be similar to the community effect described in other cell types. If a similar phenomenon exists in the embryo, or in maturity, it may contribute to the establishment of proper orientation of neurite outgrowth during development and/or injury-induced neuronal plasticity. |
| first_indexed | 2024-12-22T04:05:50Z |
| format | Article |
| id | doaj.art-5b71154272f64b8a830630dde7bce1ee |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:05:50Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5b71154272f64b8a830630dde7bce1ee2022-12-21T18:39:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e021626310.1371/journal.pone.0216263Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth.David B PettigrewCurtis B DobsonLori G IsaacsonEric C LeuthardtHeather N LilleyGeorgette L SuidanKeith A CrutcherExplants of embryonic chick sympathetic and sensory ganglia were found to exhibit asymmetric radial outgrowth of neurites under standard culture conditions with or without exogenous Nerve Growth Factor [NGF]. Opposing sides of an explant exhibited: a) differences in neurite length and, b) differences in neurite morphology. Strikingly, this asymmetry exhibited co-orientation among segregated, neighboring explants. The underlying mechanism(s) of the asymmetry and its co-orientation are not known but appear to depend on cell clustering because dissociated sympathetic neurons do not exhibit co-orientation whereas re-aggregated clusters of cells do. This emergent behavior may be similar to the community effect described in other cell types. If a similar phenomenon exists in the embryo, or in maturity, it may contribute to the establishment of proper orientation of neurite outgrowth during development and/or injury-induced neuronal plasticity.https://doi.org/10.1371/journal.pone.0216263 |
| spellingShingle | David B Pettigrew Curtis B Dobson Lori G Isaacson Eric C Leuthardt Heather N Lilley Georgette L Suidan Keith A Crutcher Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth. PLoS ONE |
| title | Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth. |
| title_full | Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth. |
| title_fullStr | Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth. |
| title_full_unstemmed | Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth. |
| title_short | Segregated neural explants exhibit co-oriented, asymmetric, neurite outgrowth. |
| title_sort | segregated neural explants exhibit co oriented asymmetric neurite outgrowth |
| url | https://doi.org/10.1371/journal.pone.0216263 |
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