Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients
Abstract Background Up to date, a well-defined microRNAs (miRNAs) profile involved in hepatocellular carcinoma (HCC) pathogenesis remains indecisive. Thus, employing miRNAs for HCC diagnosis is demanded for early therapeutic interventions. We aimed to evaluate the usage of miRNAs set related to the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43141-023-00537-2 |
| _version_ | 1827267082632298496 |
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| author | Sara M. Abdo Wafaa Gh. Shousha Amal Ahmed Mohamed Mohamed Elshobaky Mohamed Saleh Mostafa Mohamed Abdelhamid Ali |
| author_facet | Sara M. Abdo Wafaa Gh. Shousha Amal Ahmed Mohamed Mohamed Elshobaky Mohamed Saleh Mostafa Mohamed Abdelhamid Ali |
| author_sort | Sara M. Abdo |
| collection | DOAJ |
| description | Abstract Background Up to date, a well-defined microRNAs (miRNAs) profile involved in hepatocellular carcinoma (HCC) pathogenesis remains indecisive. Thus, employing miRNAs for HCC diagnosis is demanded for early therapeutic interventions. We aimed to evaluate the usage of miRNAs set related to the SuperPath: miRNAs involved in DNA damage response pathway as effective biomarkers for HCV-related HCC diagnosis. Results The study enrolled 97 patients with HCV-related HCC, 84 with hepatitis C virus (HCV), 97 with liver cirrhosis (LC), and 84 healthy individuals. Serum miRNA-23a, miRNA-203, miRNA-100-5p, and miRNA-16 were quantified using qRT-PCR experiments, AFP and routine LFTs were estimated via standard techniques. Pathway enrichment analysis along with the construction of miRNAs regulatory network were performed. With respect to healthy individuals, miRNA-203, miRNA-100-5p, and miRNA-16 were significantly downregulated in HCC, HCV, and LC groups, while miRNA-23a showed significant upregulation (p < 0.001). miRNAs exhibited significant correlations with AFP, ALT, AST, and albumin. Also, elevated levels of miRNA-23a were recognized in patients with multiple focal lesions and/or lesion size > 5 cm. Additionally, the diagnostic performance of miRNA-23a expression level at a selected cut-off value of 3.99 overtakes AFP, while expressions of miR-203, miRNA-100-5p, and miRNA-16 represent poor diagnostic outcomes. Conclusions Keeping in mind the individual variability and high level of heterogeneity in HCC, our data revealed the diagnostic value of miRNA-23a expression in HCV-related HCC patients. Further extra in silico HCC-specific microRNAs sets are demanded in diagnosis. |
| first_indexed | 2024-03-12T14:16:27Z |
| format | Article |
| id | doaj.art-5b71d740d24a413b859b477d46612ab0 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2025-03-22T04:28:05Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-5b71d740d24a413b859b477d46612ab02024-04-28T04:54:29ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202023-08-0121111110.1186/s43141-023-00537-2Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patientsSara M. Abdo0Wafaa Gh. Shousha1Amal Ahmed Mohamed2Mohamed Elshobaky3Mohamed Saleh4Mostafa Mohamed Abdelhamid Ali5Biochemistry Division, Chemistry Department, Faculty of Science, Helwan UniversityBiochemistry Division, Chemistry Department, Faculty of Science, Helwan UniversityBiochemistry Department, National Hepatology and Tropical Medicine Research Institute, Cairo UniversityInternal Medicine Department, Faculty of Medicine, Cairo UniversityInternal Medicine department, National Hepatology and Tropical Medicine Research InstituteBiochemistry Division, Chemistry Department, Faculty of Science, Helwan UniversityAbstract Background Up to date, a well-defined microRNAs (miRNAs) profile involved in hepatocellular carcinoma (HCC) pathogenesis remains indecisive. Thus, employing miRNAs for HCC diagnosis is demanded for early therapeutic interventions. We aimed to evaluate the usage of miRNAs set related to the SuperPath: miRNAs involved in DNA damage response pathway as effective biomarkers for HCV-related HCC diagnosis. Results The study enrolled 97 patients with HCV-related HCC, 84 with hepatitis C virus (HCV), 97 with liver cirrhosis (LC), and 84 healthy individuals. Serum miRNA-23a, miRNA-203, miRNA-100-5p, and miRNA-16 were quantified using qRT-PCR experiments, AFP and routine LFTs were estimated via standard techniques. Pathway enrichment analysis along with the construction of miRNAs regulatory network were performed. With respect to healthy individuals, miRNA-203, miRNA-100-5p, and miRNA-16 were significantly downregulated in HCC, HCV, and LC groups, while miRNA-23a showed significant upregulation (p < 0.001). miRNAs exhibited significant correlations with AFP, ALT, AST, and albumin. Also, elevated levels of miRNA-23a were recognized in patients with multiple focal lesions and/or lesion size > 5 cm. Additionally, the diagnostic performance of miRNA-23a expression level at a selected cut-off value of 3.99 overtakes AFP, while expressions of miR-203, miRNA-100-5p, and miRNA-16 represent poor diagnostic outcomes. Conclusions Keeping in mind the individual variability and high level of heterogeneity in HCC, our data revealed the diagnostic value of miRNA-23a expression in HCV-related HCC patients. Further extra in silico HCC-specific microRNAs sets are demanded in diagnosis.https://doi.org/10.1186/s43141-023-00537-2HCC,HCV-related HCCmiRNA-23amiRNA-203miRNA-100-5pmiRNA-16AFP |
| spellingShingle | Sara M. Abdo Wafaa Gh. Shousha Amal Ahmed Mohamed Mohamed Elshobaky Mohamed Saleh Mostafa Mohamed Abdelhamid Ali Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients Journal of Genetic Engineering and Biotechnology HCC,HCV-related HCC miRNA-23a miRNA-203 miRNA-100-5p miRNA-16 AFP |
| title | Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients |
| title_full | Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients |
| title_fullStr | Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients |
| title_full_unstemmed | Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients |
| title_short | Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients |
| title_sort | bio diagnostic performances of micrornas set related to dna damage response pathway among hepatitis c virus associated hepatocellular carcinoma patients |
| topic | HCC,HCV-related HCC miRNA-23a miRNA-203 miRNA-100-5p miRNA-16 AFP |
| url | https://doi.org/10.1186/s43141-023-00537-2 |
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