Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro
Recently, selenium nanoparticles have been drawing attention worldwide, and it is crucial to increase the stability of nano-Se. <i>Morinda officinalis</i> polysaccharides (MOP) are the main active component in <i>Morinda officinalis</i> radix. However, their low activity has...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/6/2426 |
| _version_ | 1797609914527383552 |
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| author | Mengxin Yao Yuan Deng Zhimin Zhao Depo Yang Guohui Wan Xinjun Xu |
| author_facet | Mengxin Yao Yuan Deng Zhimin Zhao Depo Yang Guohui Wan Xinjun Xu |
| author_sort | Mengxin Yao |
| collection | DOAJ |
| description | Recently, selenium nanoparticles have been drawing attention worldwide, and it is crucial to increase the stability of nano-Se. <i>Morinda officinalis</i> polysaccharides (MOP) are the main active component in <i>Morinda officinalis</i> radix. However, their low activity has limited their application. A novel selenium nanoparticle (Se-MOP) was prepared to solve these problems using MOP as a dispersant. The zeta potential was measured to evaluate the stability, and UV and ATR-FTIR were used to investigate the binding type of selenium and MOP. The morphology was observed by the TEM method. Furthermore, the inhibitory effect on five selected cancer cells (HepG2, MCF-7, AGS, PC9, and HCT8) was evaluated, showing remarkable inhibition of all five cancer cells. The mechanism of inhibition was also investigated by cell circle assay, and it was found that Se-MOP could induce cell circle G0/G1 phase arrest. Immune-enhancing activities were evaluated by measuring the proliferation and cytokines of mouse spleen lymphocytes in vitro and quantitative RT-PCR. The results indicated that single stimulation of Se-MOP and synergistic stimulation with PHA or LPS increased immune capacity and improved immune by increasing the expression of cytokines. |
| first_indexed | 2024-03-11T06:08:15Z |
| format | Article |
| id | doaj.art-5b7257a45ae543b6a35556837bfb4b37 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T06:08:15Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5b7257a45ae543b6a35556837bfb4b372023-11-17T12:50:10ZengMDPI AGMolecules1420-30492023-03-01286242610.3390/molecules28062426Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In VitroMengxin Yao0Yuan Deng1Zhimin Zhao2Depo Yang3Guohui Wan4Xinjun Xu5School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, ChinaRecently, selenium nanoparticles have been drawing attention worldwide, and it is crucial to increase the stability of nano-Se. <i>Morinda officinalis</i> polysaccharides (MOP) are the main active component in <i>Morinda officinalis</i> radix. However, their low activity has limited their application. A novel selenium nanoparticle (Se-MOP) was prepared to solve these problems using MOP as a dispersant. The zeta potential was measured to evaluate the stability, and UV and ATR-FTIR were used to investigate the binding type of selenium and MOP. The morphology was observed by the TEM method. Furthermore, the inhibitory effect on five selected cancer cells (HepG2, MCF-7, AGS, PC9, and HCT8) was evaluated, showing remarkable inhibition of all five cancer cells. The mechanism of inhibition was also investigated by cell circle assay, and it was found that Se-MOP could induce cell circle G0/G1 phase arrest. Immune-enhancing activities were evaluated by measuring the proliferation and cytokines of mouse spleen lymphocytes in vitro and quantitative RT-PCR. The results indicated that single stimulation of Se-MOP and synergistic stimulation with PHA or LPS increased immune capacity and improved immune by increasing the expression of cytokines.https://www.mdpi.com/1420-3049/28/6/2426selenium nanoparticles<i>Morinda officinalis</i> polysaccharidesanti-cancer activitiesimmune enhancement |
| spellingShingle | Mengxin Yao Yuan Deng Zhimin Zhao Depo Yang Guohui Wan Xinjun Xu Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro Molecules selenium nanoparticles <i>Morinda officinalis</i> polysaccharides anti-cancer activities immune enhancement |
| title | Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro |
| title_full | Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro |
| title_fullStr | Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro |
| title_full_unstemmed | Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro |
| title_short | Selenium Nanoparticles Based on <i>Morinda officinalis</i> Polysaccharides: Characterization, Anti-Cancer Activities, and Immune-Enhancing Activities Evaluation In Vitro |
| title_sort | selenium nanoparticles based on i morinda officinalis i polysaccharides characterization anti cancer activities and immune enhancing activities evaluation in vitro |
| topic | selenium nanoparticles <i>Morinda officinalis</i> polysaccharides anti-cancer activities immune enhancement |
| url | https://www.mdpi.com/1420-3049/28/6/2426 |
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