Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experiments
Fusarium crown rot (FCR) causes significant grain yield loss in winter cereals around the world. Breeding for resistance and/or tolerance to FCR has been slow with relatively limited success. In this study, multi-species experiments were used to demonstrate an improved method to quantify FCR infecti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1225283/full |
| _version_ | 1797756435118948352 |
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| author | Andrew Milgate Brad Baxter Steven Simpfendorfer Herdina Daniele Giblot-Ducray Nannan Yang Beverly Orchard Ben Ovenden |
| author_facet | Andrew Milgate Brad Baxter Steven Simpfendorfer Herdina Daniele Giblot-Ducray Nannan Yang Beverly Orchard Ben Ovenden |
| author_sort | Andrew Milgate |
| collection | DOAJ |
| description | Fusarium crown rot (FCR) causes significant grain yield loss in winter cereals around the world. Breeding for resistance and/or tolerance to FCR has been slow with relatively limited success. In this study, multi-species experiments were used to demonstrate an improved method to quantify FCR infection levels at plant maturity using quantitative PCR (qPCR), as well as the genotype yield retention using residual regression deviation. Using qPCR to measure FCR infection allowed a higher degree of resolution between genotypes than traditional visual stem basal browning assessments. The results were consistent across three environments with different levels of disease expression. The improved measure of FCR infection along with genotype yield retention allows for partitioning of both tolerance and partial resistance. Together these methods offer new insights into FCR partial resistance and its relative importance to tolerance in bread wheat and barley. This new approach offers a more robust, unbiased way to select for both FCR traits within breeding programs. Key message: Genetic gain for tolerance and partial resistance against Fusarium crown rot (FCR) in winter cereals has been impeded by laborious and variable visual measures of infection severity. This paper presents results of an improved method to quantify FCR infection that are strongly correlated to yield loss and reveal previously unrecognised partial resistance in barley and wheat varieties. |
| first_indexed | 2024-03-12T18:01:22Z |
| format | Article |
| id | doaj.art-5b739134019d4a25b1b3a76a2e00f53e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T18:01:22Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5b739134019d4a25b1b3a76a2e00f53e2023-08-02T10:18:34ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-08-011410.3389/fpls.2023.12252831225283Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experimentsAndrew Milgate0Brad Baxter1Steven Simpfendorfer2 Herdina3Daniele Giblot-Ducray4Nannan Yang5Beverly Orchard6Ben Ovenden7NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, AustraliaNSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, AustraliaNSW Department of Primary Industries, Tamworth Agricultural Institute, Tamworth, NSW, AustraliaSouth Australian Research and Development Institute, Plant Research Centre, Urrbrae, SA, AustraliaSouth Australian Research and Development Institute, Plant Research Centre, Urrbrae, SA, AustraliaNSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, AustraliaNSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, AustraliaNSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW, AustraliaFusarium crown rot (FCR) causes significant grain yield loss in winter cereals around the world. Breeding for resistance and/or tolerance to FCR has been slow with relatively limited success. In this study, multi-species experiments were used to demonstrate an improved method to quantify FCR infection levels at plant maturity using quantitative PCR (qPCR), as well as the genotype yield retention using residual regression deviation. Using qPCR to measure FCR infection allowed a higher degree of resolution between genotypes than traditional visual stem basal browning assessments. The results were consistent across three environments with different levels of disease expression. The improved measure of FCR infection along with genotype yield retention allows for partitioning of both tolerance and partial resistance. Together these methods offer new insights into FCR partial resistance and its relative importance to tolerance in bread wheat and barley. This new approach offers a more robust, unbiased way to select for both FCR traits within breeding programs. Key message: Genetic gain for tolerance and partial resistance against Fusarium crown rot (FCR) in winter cereals has been impeded by laborious and variable visual measures of infection severity. This paper presents results of an improved method to quantify FCR infection that are strongly correlated to yield loss and reveal previously unrecognised partial resistance in barley and wheat varieties.https://www.frontiersin.org/articles/10.3389/fpls.2023.1225283/fullFusarium crown rot (FCR)qPCRwheatbarleytolerancepartial resistance |
| spellingShingle | Andrew Milgate Brad Baxter Steven Simpfendorfer Herdina Daniele Giblot-Ducray Nannan Yang Beverly Orchard Ben Ovenden Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experiments Frontiers in Plant Science Fusarium crown rot (FCR) qPCR wheat barley tolerance partial resistance |
| title | Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experiments |
| title_full | Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experiments |
| title_fullStr | Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experiments |
| title_full_unstemmed | Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experiments |
| title_short | Improved quantification of Fusarium pseudograminearum (Fusarium crown rot) using qPCR measurement of infection in multi-species winter cereal experiments |
| title_sort | improved quantification of fusarium pseudograminearum fusarium crown rot using qpcr measurement of infection in multi species winter cereal experiments |
| topic | Fusarium crown rot (FCR) qPCR wheat barley tolerance partial resistance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1225283/full |
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