Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose.
We previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched α-glucan surface polysaccharide. This glucan, surface attached...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3341381?pdf=render |
| _version_ | 1818570839016079360 |
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| author | Susana Merino Lamiaa Bouamama Yuriy A Knirel Sofya N Senchenkova Miguel Regué Juan M Tomás |
| author_facet | Susana Merino Lamiaa Bouamama Yuriy A Knirel Sofya N Senchenkova Miguel Regué Juan M Tomás |
| author_sort | Susana Merino |
| collection | DOAJ |
| description | We previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched α-glucan surface polysaccharide. This glucan, surface attached through the O-antigen ligase (WaaL), is common to the mesophilic Aeromonas strains tested. The Aeromonas glucan is produced by the action of the glycogen synthase (GlgA) and the UDP-Glc pyrophosphorylase (GlgC), the latter wrongly indicated as an ADP-Glc pyrophosphorylase in the Aeromonas genomes available. The Aeromonas glycogen synthase is able to react with UDP or ADP-glucose, which is not the case of E. coli glycogen synthase only reacting with ADP-glucose. The Aeromonas surface glucan has a role enhancing biofilm formation. Finally, for the first time to our knowledge, a clear preference on behalf of bacterial survival and pathogenesis is observed when choosing to produce one or other surface saccharide molecules to produce (lipopolysaccharide core or glucan). |
| first_indexed | 2024-12-14T13:47:09Z |
| format | Article |
| id | doaj.art-5b74b7de284e432492e86075cd537e0e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T13:47:09Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5b74b7de284e432492e86075cd537e0e2022-12-21T22:59:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3570710.1371/journal.pone.0035707Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose.Susana MerinoLamiaa BouamamaYuriy A KnirelSofya N SenchenkovaMiguel ReguéJuan M TomásWe previously reported that A. hydrophila GalU mutants were still able to produce UDP-glucose introduced as a glucose residue in their lipopolysaccharide core. In this study, we found the unique origin of this UDP-glucose from a branched α-glucan surface polysaccharide. This glucan, surface attached through the O-antigen ligase (WaaL), is common to the mesophilic Aeromonas strains tested. The Aeromonas glucan is produced by the action of the glycogen synthase (GlgA) and the UDP-Glc pyrophosphorylase (GlgC), the latter wrongly indicated as an ADP-Glc pyrophosphorylase in the Aeromonas genomes available. The Aeromonas glycogen synthase is able to react with UDP or ADP-glucose, which is not the case of E. coli glycogen synthase only reacting with ADP-glucose. The Aeromonas surface glucan has a role enhancing biofilm formation. Finally, for the first time to our knowledge, a clear preference on behalf of bacterial survival and pathogenesis is observed when choosing to produce one or other surface saccharide molecules to produce (lipopolysaccharide core or glucan).http://europepmc.org/articles/PMC3341381?pdf=render |
| spellingShingle | Susana Merino Lamiaa Bouamama Yuriy A Knirel Sofya N Senchenkova Miguel Regué Juan M Tomás Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose. PLoS ONE |
| title | Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose. |
| title_full | Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose. |
| title_fullStr | Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose. |
| title_full_unstemmed | Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose. |
| title_short | Aeromonas surface glucan attached through the O-antigen ligase represents a new way to obtain UDP-glucose. |
| title_sort | aeromonas surface glucan attached through the o antigen ligase represents a new way to obtain udp glucose |
| url | http://europepmc.org/articles/PMC3341381?pdf=render |
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