Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved]
Background: Despite technological advances, how specific cell types are involved in brain function remains shrouded in mystery. Further, little is known about the contribution of different ion channel currents to cell excitability across different neuronal subtypes and their dendritic compartments i...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2020-06-01
|
| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/9-180/v2 |
| _version_ | 1819048401529995264 |
|---|---|
| author | Alexandre Guet-McCreight Frances K. Skinner |
| author_facet | Alexandre Guet-McCreight Frances K. Skinner |
| author_sort | Alexandre Guet-McCreight |
| collection | DOAJ |
| description | Background: Despite technological advances, how specific cell types are involved in brain function remains shrouded in mystery. Further, little is known about the contribution of different ion channel currents to cell excitability across different neuronal subtypes and their dendritic compartments in vivo. The picture that we do have is largely based on somatic recordings performed in vitro. Uncovering dendritic ion channel current contributions in neuron subtypes that represent a minority of the neuronal population is not currently a feasible task using purely experimental means. Methods: We employ two morphologically-detailed multi-compartment models of a specific type of inhibitory interneuron, the oriens lacunosum moleculare (OLM) cell. The OLM cell is a well-studied cell type in CA1 hippocampus that is important in gating sensory and contextual information. We create in vivo-like states for these cellular models by including levels of synaptic bombardment that would occur in vivo. Using visualization tools and analyses we assess the ion channel current contribution profile across the different somatic and dendritic compartments of the models. Results: We identify changes in dendritic excitability, ion channel current contributions and co-activation patterns between in vitro and in vivo-like states. Primarily, we find that the relative timing between ion channel currents are mostly invariant between states, but exhibit changes in magnitudes and decreased propagation across dendritic compartments. We also find enhanced dendritic hyperpolarization-activated cyclic nucleotide-gated channel (h-channel) activation during in vivo-like states, which suggests that dendritically located h-channels are functionally important in altering signal propagation in the behaving animal. Conclusions: Overall, we have demonstrated, using computational modelling, the dynamical changes that can occur to ion channel mechanisms governing neuronal spiking. Simultaneous access to dendritic compartments during simulated in vivo states shows that the magnitudes of some ion channel current contributions are differentially altered during in vivo-like states relative to in vitro. |
| first_indexed | 2024-12-21T11:15:40Z |
| format | Article |
| id | doaj.art-5b7511ca597e4e9097c37029e10a7504 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-21T11:15:40Z |
| publishDate | 2020-06-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-5b7511ca597e4e9097c37029e10a75042022-12-21T19:05:55ZengF1000 Research LtdF1000Research2046-14022020-06-01910.12688/f1000research.22584.227205Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved]Alexandre Guet-McCreight0Frances K. Skinner1Department of Physiology, University of Toronto, Toronto, ON, CanadaKrembil Research Institute, University Health Network, Toronto, ON, M5T 0S8, CanadaBackground: Despite technological advances, how specific cell types are involved in brain function remains shrouded in mystery. Further, little is known about the contribution of different ion channel currents to cell excitability across different neuronal subtypes and their dendritic compartments in vivo. The picture that we do have is largely based on somatic recordings performed in vitro. Uncovering dendritic ion channel current contributions in neuron subtypes that represent a minority of the neuronal population is not currently a feasible task using purely experimental means. Methods: We employ two morphologically-detailed multi-compartment models of a specific type of inhibitory interneuron, the oriens lacunosum moleculare (OLM) cell. The OLM cell is a well-studied cell type in CA1 hippocampus that is important in gating sensory and contextual information. We create in vivo-like states for these cellular models by including levels of synaptic bombardment that would occur in vivo. Using visualization tools and analyses we assess the ion channel current contribution profile across the different somatic and dendritic compartments of the models. Results: We identify changes in dendritic excitability, ion channel current contributions and co-activation patterns between in vitro and in vivo-like states. Primarily, we find that the relative timing between ion channel currents are mostly invariant between states, but exhibit changes in magnitudes and decreased propagation across dendritic compartments. We also find enhanced dendritic hyperpolarization-activated cyclic nucleotide-gated channel (h-channel) activation during in vivo-like states, which suggests that dendritically located h-channels are functionally important in altering signal propagation in the behaving animal. Conclusions: Overall, we have demonstrated, using computational modelling, the dynamical changes that can occur to ion channel mechanisms governing neuronal spiking. Simultaneous access to dendritic compartments during simulated in vivo states shows that the magnitudes of some ion channel current contributions are differentially altered during in vivo-like states relative to in vitro.https://f1000research.com/articles/9-180/v2 |
| spellingShingle | Alexandre Guet-McCreight Frances K. Skinner Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved] F1000Research |
| title | Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved] |
| title_full | Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved] |
| title_fullStr | Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved] |
| title_full_unstemmed | Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved] |
| title_short | Computationally going where experiments cannot: a dynamical assessment of dendritic ion channel currents during in vivo-like states [version 2; peer review: 2 approved] |
| title_sort | computationally going where experiments cannot a dynamical assessment of dendritic ion channel currents during in vivo like states version 2 peer review 2 approved |
| url | https://f1000research.com/articles/9-180/v2 |
| work_keys_str_mv | AT alexandreguetmccreight computationallygoingwhereexperimentscannotadynamicalassessmentofdendriticionchannelcurrentsduringinvivolikestatesversion2peerreview2approved AT franceskskinner computationallygoingwhereexperimentscannotadynamicalassessmentofdendriticionchannelcurrentsduringinvivolikestatesversion2peerreview2approved |