Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman Spectroscopy
The prevalence of antimicrobial-resistant bacteria has become a major challenge worldwide. Methicillin-resistant Staphylococcus aureus (MRSA)—a leading cause of infections—forms biofilms on polymeric medical devices and implants, increasing their resistance to antibiotics. Antibiotic administration...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/7/1937 |
| _version_ | 1797587791603826688 |
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| author | Jina Kim Young-Won Chin |
| author_facet | Jina Kim Young-Won Chin |
| author_sort | Jina Kim |
| collection | DOAJ |
| description | The prevalence of antimicrobial-resistant bacteria has become a major challenge worldwide. Methicillin-resistant Staphylococcus aureus (MRSA)—a leading cause of infections—forms biofilms on polymeric medical devices and implants, increasing their resistance to antibiotics. Antibiotic administration before biofilm formation is crucial. Raman spectroscopy was used to assess MRSA biofilm development on solid culture media from 0 to 48 h. Biofilm formation was monitored by measuring DNA/RNA-associated Raman peaks and protein/lipid-associated peaks. The search for an antimicrobial agent against MRSA biofilm revealed that Eugenol was a promising candidate as it showed significant potential for breaking down biofilm. Eugenol was applied at different times to test the optimal time for inhibiting MRSA biofilms, and the Raman spectrum showed that the first 5 h of biofilm formation was the most antibiotic-sensitive time. This study investigated the performance of Raman spectroscopy coupled with principal component analysis (PCA) to identify planktonic bacteria from biofilm conglomerates. Raman analysis, microscopic observation, and quantification of the biofilm growth curve indicated early adhesion from 5 to 10 h of the incubation time. Therefore, Raman spectroscopy can help in monitoring biofilm formation on a solid culture medium and performing rapid antibiofilm assessments with new antibiotics during the early stages of the procedure. |
| first_indexed | 2024-03-11T00:43:54Z |
| format | Article |
| id | doaj.art-5b7a40dd420442f4b320a0281b2017c4 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T00:43:54Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-5b7a40dd420442f4b320a0281b2017c42023-11-18T20:55:56ZengMDPI AGPharmaceutics1999-49232023-07-01157193710.3390/pharmaceutics15071937Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman SpectroscopyJina Kim0Young-Won Chin1College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of KoreaCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of KoreaThe prevalence of antimicrobial-resistant bacteria has become a major challenge worldwide. Methicillin-resistant Staphylococcus aureus (MRSA)—a leading cause of infections—forms biofilms on polymeric medical devices and implants, increasing their resistance to antibiotics. Antibiotic administration before biofilm formation is crucial. Raman spectroscopy was used to assess MRSA biofilm development on solid culture media from 0 to 48 h. Biofilm formation was monitored by measuring DNA/RNA-associated Raman peaks and protein/lipid-associated peaks. The search for an antimicrobial agent against MRSA biofilm revealed that Eugenol was a promising candidate as it showed significant potential for breaking down biofilm. Eugenol was applied at different times to test the optimal time for inhibiting MRSA biofilms, and the Raman spectrum showed that the first 5 h of biofilm formation was the most antibiotic-sensitive time. This study investigated the performance of Raman spectroscopy coupled with principal component analysis (PCA) to identify planktonic bacteria from biofilm conglomerates. Raman analysis, microscopic observation, and quantification of the biofilm growth curve indicated early adhesion from 5 to 10 h of the incubation time. Therefore, Raman spectroscopy can help in monitoring biofilm formation on a solid culture medium and performing rapid antibiofilm assessments with new antibiotics during the early stages of the procedure.https://www.mdpi.com/1999-4923/15/7/1937Methicillin-resistant <i>Staphylococcus aureus</i>biofilmantimicrobial agenteugenolRaman spectroscopy |
| spellingShingle | Jina Kim Young-Won Chin Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman Spectroscopy Pharmaceutics Methicillin-resistant <i>Staphylococcus aureus</i> biofilm antimicrobial agent eugenol Raman spectroscopy |
| title | Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman Spectroscopy |
| title_full | Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman Spectroscopy |
| title_fullStr | Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman Spectroscopy |
| title_full_unstemmed | Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman Spectroscopy |
| title_short | Antimicrobial Agent against Methicillin-Resistant <i>Staphylococcus aureus</i> Biofilm Monitored Using Raman Spectroscopy |
| title_sort | antimicrobial agent against methicillin resistant i staphylococcus aureus i biofilm monitored using raman spectroscopy |
| topic | Methicillin-resistant <i>Staphylococcus aureus</i> biofilm antimicrobial agent eugenol Raman spectroscopy |
| url | https://www.mdpi.com/1999-4923/15/7/1937 |
| work_keys_str_mv | AT jinakim antimicrobialagentagainstmethicillinresistantistaphylococcusaureusibiofilmmonitoredusingramanspectroscopy AT youngwonchin antimicrobialagentagainstmethicillinresistantistaphylococcusaureusibiofilmmonitoredusingramanspectroscopy |