| Summary: | In this study we conducted an ichthyofaunistic survey in the Pirapó River drainage, upper Paraná River basin, based on voucher specimens deposited in the Coleção Ictiológica do Nupélia/UEM. We reported a total of six orders, 23 families, and 76 species, in which four of them were classified as allochthonous and two as exotics. Twenty-eight percent of these species were recorded only for tributaries, whereas the commercially important migratory species, Dourado - Salminus brasiliensis (Cuvier, 1816), was recorded in the main channel, indicating that the Pirapó River, still free from impoundments, may be one of the last rivers in the upper Paraná River basin that can be used to protect migratory fish species.
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