Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesis
ObjectiveN6-Methyladenosine (m6A) modification is of great importance in both the pathological conditions and physiological process. The m6A single nucleotide polymorphisms (SNPs) are associated with cardiovascular diseases including coronary artery disease, heart failure. However, it is unclear whe...
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| Format: | Article |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1152851/full |
| _version_ | 1797819812193239040 |
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| author | Yan Huang Yan Huang Yan Huang Yuqian Tan Yuan Yao Yuan Yao Yuan Yao Linglong Gu Liusong Huang Tao Song Tao Song Tao Song |
| author_facet | Yan Huang Yan Huang Yan Huang Yuqian Tan Yuan Yao Yuan Yao Yuan Yao Linglong Gu Liusong Huang Tao Song Tao Song Tao Song |
| author_sort | Yan Huang |
| collection | DOAJ |
| description | ObjectiveN6-Methyladenosine (m6A) modification is of great importance in both the pathological conditions and physiological process. The m6A single nucleotide polymorphisms (SNPs) are associated with cardiovascular diseases including coronary artery disease, heart failure. However, it is unclear whether m6A-SNPs are involved in atrial fibrillation (AF). Here, we aimed to explore the relationship between m6A-SNPs and AF.MethodThe relationship between m6A-SNPs and AF was evaluated by analyzing the AF genome-wide association study (GWAS) and m6A-SNPs annotated by the m6AVar database. Further, eQTL and gene differential expression analysis were performed to confirm the association between these identified m6A-SNPs and their target genes in the development of AF. Moreover, we did the GO enrichment analysis to figure out the potential functions of these m6A-SNPs affected genes.ResultTotally, 105 m6A-SNPs were identified to be significantly associated with AF (FDR < 0.05), among which 7 showed significant eQTL signals on local genes in the atrial appendage. By using four public AF gene expression datasets, we identified genes SYNE2, USP36, and THAP9 containing SNPs rs35648226, rs900349, and rs1047564 were differentially expressed in AF population. Further, SNPs rs35648226 and rs1047564 are potentially associated with AF by affecting m6A modification and both of them might have an interaction with RNA-binding protein, PABPC1.ConclusionIn summary, we identified m6A-SNPs associated with AF. Our study provided new insights into AF development as well as AF therapeutic target. |
| first_indexed | 2024-03-13T09:28:08Z |
| format | Article |
| id | doaj.art-5b81d9a9ad7142ae8193e3a648c68ea4 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-03-13T09:28:08Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-5b81d9a9ad7142ae8193e3a648c68ea42023-05-26T04:50:30ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2023-05-011010.3389/fcvm.2023.11528511152851Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesisYan Huang0Yan Huang1Yan Huang2Yuqian Tan3Yuan Yao4Yuan Yao5Yuan Yao6Linglong Gu7Liusong Huang8Tao Song9Tao Song10Tao Song11Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaCardiovascular Research Institute of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Cardiology, Wuhan, ChinaDepartment of Health Toxicology, Key Laboratory for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaCardiovascular Research Institute of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Cardiology, Wuhan, ChinaDepartment of Health Toxicology, Key Laboratory for Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaCollege of Software Engineering, Maanshan Teacher's College, Maanshan, ChinaDepartment of Cardiology, Renmin Hospital of Wuhan University, Wuhan, ChinaCardiovascular Research Institute of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Cardiology, Wuhan, ChinaObjectiveN6-Methyladenosine (m6A) modification is of great importance in both the pathological conditions and physiological process. The m6A single nucleotide polymorphisms (SNPs) are associated with cardiovascular diseases including coronary artery disease, heart failure. However, it is unclear whether m6A-SNPs are involved in atrial fibrillation (AF). Here, we aimed to explore the relationship between m6A-SNPs and AF.MethodThe relationship between m6A-SNPs and AF was evaluated by analyzing the AF genome-wide association study (GWAS) and m6A-SNPs annotated by the m6AVar database. Further, eQTL and gene differential expression analysis were performed to confirm the association between these identified m6A-SNPs and their target genes in the development of AF. Moreover, we did the GO enrichment analysis to figure out the potential functions of these m6A-SNPs affected genes.ResultTotally, 105 m6A-SNPs were identified to be significantly associated with AF (FDR < 0.05), among which 7 showed significant eQTL signals on local genes in the atrial appendage. By using four public AF gene expression datasets, we identified genes SYNE2, USP36, and THAP9 containing SNPs rs35648226, rs900349, and rs1047564 were differentially expressed in AF population. Further, SNPs rs35648226 and rs1047564 are potentially associated with AF by affecting m6A modification and both of them might have an interaction with RNA-binding protein, PABPC1.ConclusionIn summary, we identified m6A-SNPs associated with AF. Our study provided new insights into AF development as well as AF therapeutic target.https://www.frontiersin.org/articles/10.3389/fcvm.2023.1152851/fullN6-methyladenosine methylationatrial fibrillationsingle nucleotide polymorphismgenome-wide association study (GWAS)m6A modification |
| spellingShingle | Yan Huang Yan Huang Yan Huang Yuqian Tan Yuan Yao Yuan Yao Yuan Yao Linglong Gu Liusong Huang Tao Song Tao Song Tao Song Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesis Frontiers in Cardiovascular Medicine N6-methyladenosine methylation atrial fibrillation single nucleotide polymorphism genome-wide association study (GWAS) m6A modification |
| title | Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesis |
| title_full | Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesis |
| title_fullStr | Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesis |
| title_full_unstemmed | Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesis |
| title_short | Genome-wide detection of m6A-associated SNPs in atrial fibrillation pathogenesis |
| title_sort | genome wide detection of m6a associated snps in atrial fibrillation pathogenesis |
| topic | N6-methyladenosine methylation atrial fibrillation single nucleotide polymorphism genome-wide association study (GWAS) m6A modification |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2023.1152851/full |
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