Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry
Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44,...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/9/12/955 |
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| author | Marek Feith Yuecheng Zhang Jenny L. Persson Jan Balvan Zahra El-Schich Anette Gjörloff Wingren |
| author_facet | Marek Feith Yuecheng Zhang Jenny L. Persson Jan Balvan Zahra El-Schich Anette Gjörloff Wingren |
| author_sort | Marek Feith |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. In this study, we intended to mimic the environment for metastasizing cells. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers. |
| first_indexed | 2024-03-09T15:57:21Z |
| format | Article |
| id | doaj.art-5b8574ed38414a39a7f0bafcdbb938d4 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-09T15:57:21Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-5b8574ed38414a39a7f0bafcdbb938d42023-11-24T17:25:22ZengMDPI AGPhotonics2304-67322022-12-0191295510.3390/photonics9120955Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic CytometryMarek Feith0Yuecheng Zhang1Jenny L. Persson2Jan Balvan3Zahra El-Schich4Anette Gjörloff Wingren5Department of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, SwedenDepartment of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, SwedenDepartment of Pathophysiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech RepublicDepartment of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, SwedenDepartment of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, SwedenColorectal cancer (CRC) is the second most metastatic disease with the majority of cases detected in Western countries. Metastases are formed by circulating altered phenotype tumor cells causing 20% of CRC related deaths. Metastatic cells may show higher expression of surface molecules such as CD44, and changes in morphological properties are associated with increased invasiveness and poor prognosis. In this study, we intended to mimic the environment for metastasizing cells. Here, we used digital holographic cytometry (DHC) analysis to determine cellular morphological properties of three metastatic and two non-metastatic colorectal cancer cell lines to show differences in morphology between the CRC cells and peripheral blood mononuclear cells (PBMCs). By establishing differences in cell area, cell thickness, cell volume, and cell irregularity even when the CRC cells were in minority (5% out of PBMCs), DHC does discriminate between CRC cells and the PBMCs in vitro. We also analyzed the epithelial marker EpCAM and migration marker CD44 using flow cytometry and demonstrate that the CRC cell lines and PBMC cells differ in EpCAM and CD44 expression. Here, we present DHC as a new powerful tool in discriminating cells of different sizes in suspension together with a combination of biomarkers.https://www.mdpi.com/2304-6732/9/12/955cell morphologyCD44circulating tumor cellcolorectal cancerdigital holographic cytometryEpCAM |
| spellingShingle | Marek Feith Yuecheng Zhang Jenny L. Persson Jan Balvan Zahra El-Schich Anette Gjörloff Wingren Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry Photonics cell morphology CD44 circulating tumor cell colorectal cancer digital holographic cytometry EpCAM |
| title | Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry |
| title_full | Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry |
| title_fullStr | Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry |
| title_full_unstemmed | Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry |
| title_short | Circulating Tumor Cell Models Mimicking Metastasizing Cells In Vitro: Discrimination of Colorectal Cancer Cells and White Blood Cells Using Digital Holographic Cytometry |
| title_sort | circulating tumor cell models mimicking metastasizing cells in vitro discrimination of colorectal cancer cells and white blood cells using digital holographic cytometry |
| topic | cell morphology CD44 circulating tumor cell colorectal cancer digital holographic cytometry EpCAM |
| url | https://www.mdpi.com/2304-6732/9/12/955 |
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