Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis
Eriocheir sinensis larva normally experiences 11 stages. The reduced abdomen folded beneath the thorax is the most prominent characteristic of morphological alteration from megalopa to juvenile crab. Up to date, the molecular mechanisms of brachyurization remain a mystery. Here, transcriptome librar...
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| Format: | Article |
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Elsevier
2023-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022040245 |
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| author | Yunxia Yang Fangcao Jin Wanyi Liu Guangming Huo Feng Zhou Jie Yan Kaiya Zhou Peng Li |
| author_facet | Yunxia Yang Fangcao Jin Wanyi Liu Guangming Huo Feng Zhou Jie Yan Kaiya Zhou Peng Li |
| author_sort | Yunxia Yang |
| collection | DOAJ |
| description | Eriocheir sinensis larva normally experiences 11 stages. The reduced abdomen folded beneath the thorax is the most prominent characteristic of morphological alteration from megalopa to juvenile crab. Up to date, the molecular mechanisms of brachyurization remain a mystery. Here, transcriptome library, digital gene expression (DGE) libraries and proteome libraries at two developmental stages [the megalopa stage of E. sinensis (stage M) and the first stage of juvenile crab (stage J1)] of the Chinese mitten crab larva were constructed for RNA sequencing and iTRAQ approaches followed by bioinformatics analysis, respectively. In total, 1106 genes and 871 proteins were differentially expressed between the stage M and stage J1. Moreover, several important pathways were identified, including biosynthesis of secondary metabolites, metabolic pathways, focal adhesion, and some disease pathways. Besides, muscle contraction, oxidative phosphorylation, calcium signaling, PI3K-Akt, DNA replication pathway, and integrin signaling pathway also had important functions in brachyurization process. Furthermore, the components, actin, actin-related protein, collagens, filamin-A/B, laminin, integrins, paxillin, and fibronectin had up-regulated expression levels in M stage compared to J1 stage. |
| first_indexed | 2024-04-10T17:46:22Z |
| format | Article |
| id | doaj.art-5b871091077043d2ae39cea7ef0a56a0 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T17:46:22Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-5b871091077043d2ae39cea7ef0a56a02023-02-03T04:58:49ZengElsevierHeliyon2405-84402023-01-0191e12736Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensisYunxia Yang0Fangcao Jin1Wanyi Liu2Guangming Huo3Feng Zhou4Jie Yan5Kaiya Zhou6Peng Li7School of Fishery, Zhejiang Ocean University, Zhoushan 316022, PR ChinaJiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR ChinaJiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR ChinaSchool of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, PR ChinaSchool of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, PR ChinaJiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR ChinaJiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR ChinaJiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, PR China; Corresponding author. College of Life Sciences, Nanjing Normal University, No. 1 Wenyuan Road, Nanjing 210023, Jiangsu, PR China.Eriocheir sinensis larva normally experiences 11 stages. The reduced abdomen folded beneath the thorax is the most prominent characteristic of morphological alteration from megalopa to juvenile crab. Up to date, the molecular mechanisms of brachyurization remain a mystery. Here, transcriptome library, digital gene expression (DGE) libraries and proteome libraries at two developmental stages [the megalopa stage of E. sinensis (stage M) and the first stage of juvenile crab (stage J1)] of the Chinese mitten crab larva were constructed for RNA sequencing and iTRAQ approaches followed by bioinformatics analysis, respectively. In total, 1106 genes and 871 proteins were differentially expressed between the stage M and stage J1. Moreover, several important pathways were identified, including biosynthesis of secondary metabolites, metabolic pathways, focal adhesion, and some disease pathways. Besides, muscle contraction, oxidative phosphorylation, calcium signaling, PI3K-Akt, DNA replication pathway, and integrin signaling pathway also had important functions in brachyurization process. Furthermore, the components, actin, actin-related protein, collagens, filamin-A/B, laminin, integrins, paxillin, and fibronectin had up-regulated expression levels in M stage compared to J1 stage.http://www.sciencedirect.com/science/article/pii/S2405844022040245Eriocheir sinensisBrachyurizationTranscriptomeProteomeDigital gene expression |
| spellingShingle | Yunxia Yang Fangcao Jin Wanyi Liu Guangming Huo Feng Zhou Jie Yan Kaiya Zhou Peng Li Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis Heliyon Eriocheir sinensis Brachyurization Transcriptome Proteome Digital gene expression |
| title | Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis |
| title_full | Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis |
| title_fullStr | Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis |
| title_full_unstemmed | Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis |
| title_short | Comparative transcriptome, digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in Eriocheir sinensis |
| title_sort | comparative transcriptome digital gene expression and proteome profiling analyses provide insights into the brachyurization from the megalopa to the first juvenile in eriocheir sinensis |
| topic | Eriocheir sinensis Brachyurization Transcriptome Proteome Digital gene expression |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022040245 |
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