Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil
Plant seeds have long been promoted as a production platform for novel fatty acids such as the ω3 long-chain (≥ C20) polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) commonly found in fish oil. In this article we describe the creation of a canola (Brassica napus...
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00727/full |
| _version_ | 1818543553564901376 |
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| author | James R. Petrie Xue-Rong Zhou Antonio Leonforte Jason McAllister Pushkar Shrestha Yoko Kennedy Srinivas Belide Greg Buzza Nelson Gororo Wenxiang Gao Geraldine Lester Maged P. Mansour Roger J. Mulder Qing Liu Lijun Tian Claudio Silva Noel O. I. Cogan Peter D. Nichols Allan G. Green Robert de Feyter Malcolm D. Devine Surinder P. Singh |
| author_facet | James R. Petrie Xue-Rong Zhou Antonio Leonforte Jason McAllister Pushkar Shrestha Yoko Kennedy Srinivas Belide Greg Buzza Nelson Gororo Wenxiang Gao Geraldine Lester Maged P. Mansour Roger J. Mulder Qing Liu Lijun Tian Claudio Silva Noel O. I. Cogan Peter D. Nichols Allan G. Green Robert de Feyter Malcolm D. Devine Surinder P. Singh |
| author_sort | James R. Petrie |
| collection | DOAJ |
| description | Plant seeds have long been promoted as a production platform for novel fatty acids such as the ω3 long-chain (≥ C20) polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) commonly found in fish oil. In this article we describe the creation of a canola (Brassica napus) variety producing fish oil-like levels of DHA in the seed. This was achieved by the introduction of a microalgal/yeast transgenic pathway of seven consecutive enzymatic steps which converted the native substrate oleic acid to α-linolenic acid and, subsequently, to EPA, docosapentaenoic acid (DPA) and DHA. This paper describes construct design and evaluation, plant transformation, event selection, field testing in a wide range of environments, and oil profile stability of the transgenic seed. The stable, high-performing event NS-B50027-4 produced fish oil-like levels of DHA (9–11%) in open field trials of T3 to T7 generation plants in several locations in Australia and Canada. This study also describes the highest seed DHA levels reported thus far and is one of the first examples of a deregulated genetically modified crop with clear health benefits to the consumer. |
| first_indexed | 2024-12-11T22:36:54Z |
| format | Article |
| id | doaj.art-5b8722e11123492dbe1243afe0b055b4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T22:36:54Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5b8722e11123492dbe1243afe0b055b42022-12-22T00:47:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00727537701Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed OilJames R. Petrie0Xue-Rong Zhou1Antonio Leonforte2Jason McAllister3Pushkar Shrestha4Yoko Kennedy5Srinivas Belide6Greg Buzza7Nelson Gororo8Wenxiang Gao9Geraldine Lester10Maged P. Mansour11Roger J. Mulder12Qing Liu13Lijun Tian14Claudio Silva15Noel O. I. Cogan16Peter D. Nichols17Allan G. Green18Robert de Feyter19Malcolm D. Devine20Surinder P. Singh21CSIRO Agriculture and Food, Canberra, ACT, AustraliaCSIRO Agriculture and Food, Canberra, ACT, AustraliaNuseed Pty Ltd., Horsham, VIC, AustraliaNuseed Pty Ltd., Laverton North, VIC, AustraliaCSIRO Agriculture and Food, Canberra, ACT, AustraliaCSIRO Agriculture and Food, Canberra, ACT, AustraliaCSIRO Agriculture and Food, Canberra, ACT, AustraliaNuseed Pty Ltd., Horsham, VIC, AustraliaNuseed Pty Ltd., Horsham, VIC, AustraliaNuseed Americas Inc., Woodland, CA, United StatesCSIRO Agriculture and Food, Canberra, ACT, AustraliaCSIRO Agriculture and Food, Werribee, VIC, AustraliaCSIRO Manufacturing, Clayton, VIC, AustraliaCSIRO Agriculture and Food, Canberra, ACT, AustraliaCSIRO Agriculture and Food, Canberra, ACT, AustraliaNufarm Ltd., Laverton North, VIC, AustraliaDepartment of Primary Industries, Melbourne, VIC, AustraliaCSIRO Ocean & Atmosphere, Hobart, TAS, AustraliaCSIRO Agriculture and Food, Canberra, ACT, AustraliaCSIRO Agriculture and Food, Canberra, ACT, Australia0Nuseed Global, Calgary, AB, CanadaCSIRO Agriculture and Food, Canberra, ACT, AustraliaPlant seeds have long been promoted as a production platform for novel fatty acids such as the ω3 long-chain (≥ C20) polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) commonly found in fish oil. In this article we describe the creation of a canola (Brassica napus) variety producing fish oil-like levels of DHA in the seed. This was achieved by the introduction of a microalgal/yeast transgenic pathway of seven consecutive enzymatic steps which converted the native substrate oleic acid to α-linolenic acid and, subsequently, to EPA, docosapentaenoic acid (DPA) and DHA. This paper describes construct design and evaluation, plant transformation, event selection, field testing in a wide range of environments, and oil profile stability of the transgenic seed. The stable, high-performing event NS-B50027-4 produced fish oil-like levels of DHA (9–11%) in open field trials of T3 to T7 generation plants in several locations in Australia and Canada. This study also describes the highest seed DHA levels reported thus far and is one of the first examples of a deregulated genetically modified crop with clear health benefits to the consumer.https://www.frontiersin.org/article/10.3389/fpls.2020.00727/fullω3 long-chain polyunsaturated fatty acidDHAcanolaelite eventfield trial |
| spellingShingle | James R. Petrie Xue-Rong Zhou Antonio Leonforte Jason McAllister Pushkar Shrestha Yoko Kennedy Srinivas Belide Greg Buzza Nelson Gororo Wenxiang Gao Geraldine Lester Maged P. Mansour Roger J. Mulder Qing Liu Lijun Tian Claudio Silva Noel O. I. Cogan Peter D. Nichols Allan G. Green Robert de Feyter Malcolm D. Devine Surinder P. Singh Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil Frontiers in Plant Science ω3 long-chain polyunsaturated fatty acid DHA canola elite event field trial |
| title | Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil |
| title_full | Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil |
| title_fullStr | Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil |
| title_full_unstemmed | Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil |
| title_short | Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil |
| title_sort | development of a brassica napus canola crop containing fish oil like levels of dha in the seed oil |
| topic | ω3 long-chain polyunsaturated fatty acid DHA canola elite event field trial |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00727/full |
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