Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic Features
Microvascular Doppler (MicroV) is a new-generation Doppler technique developed by Esaote (Esaote s.p.a., Genova, Italy), which is able to visualize small and low-flow vessels through a suppression of interfering signals. MicroV uses advanced filters that are able to differentiate tissue artifacts fr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/21/5335 |
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| author | Giuseppe Roberto Giammalva Anna Viola Rosario Maugeri Kevin Giardina Rina Di Bonaventura Sofia Musso Lara Brunasso Santiago Cepeda Giuseppe Maria Della Pepa Alba Scerrati Giorgio Mantovani Gianluca Ferini Rosa Maria Gerardi Maria Angela Pino Giuseppe Emmanuele Umana Luca Denaro Alessio Albanese Domenico Gerardo Iacopino |
| author_facet | Giuseppe Roberto Giammalva Anna Viola Rosario Maugeri Kevin Giardina Rina Di Bonaventura Sofia Musso Lara Brunasso Santiago Cepeda Giuseppe Maria Della Pepa Alba Scerrati Giorgio Mantovani Gianluca Ferini Rosa Maria Gerardi Maria Angela Pino Giuseppe Emmanuele Umana Luca Denaro Alessio Albanese Domenico Gerardo Iacopino |
| author_sort | Giuseppe Roberto Giammalva |
| collection | DOAJ |
| description | Microvascular Doppler (MicroV) is a new-generation Doppler technique developed by Esaote (Esaote s.p.a., Genova, Italy), which is able to visualize small and low-flow vessels through a suppression of interfering signals. MicroV uses advanced filters that are able to differentiate tissue artifacts from low-speed blood flows; by exploiting the space–time coherence information, these filters can selectively suppress tissue components, preserving the signal coming from the microvascular flow. This technique is clinically applied to the study of the vascularization of parenchymatous lesions, often with better diagnostic accuracy than color/power Doppler techniques. The aim of this paper is to develop a reproducible protocol for the recording and collection of MicroV intraoperative ultrasound images by the use of a capable intraoperative ultrasound machine and post-processing aimed at evaluation of brain-tumor microvascularization through the analysis of radiomic features. The proposed protocol has been internally validated on eight patients and will be firstly applied to patients affected by WHO grade IV astrocytoma (glioblastoma—GBM) candidates for craniotomy and lesion removal. In a further stage, it will be generally applied to patients with primary or metastatic brain tumors. IOUS is performed before durotomy. Tumor microvascularization is evaluated using the MicroV Doppler technique and IOUS images are recorded, stored, and post-processed. IOUS images are remotely stored on the BraTIoUS database, which will promote international cooperation and multicentric analysis. Processed images and texture radiomic features are analyzed post-operatively using ImageJ, a free scientific image-analysis software based on the Sun-Java platform. Post-processing protocol is further described in-depth. The study of tumor microvascularization through advanced IOUS techniques such as MicroV could represent, in the future, a non-invasive and real-time method for intraoperative predictive evaluation of the tumor features. This evaluation could finally result in a deeper knowledge of brain-tumor behavior and in the on-going adaptation of the surgery with the improvement of surgical outcomes. |
| first_indexed | 2024-03-09T19:12:13Z |
| format | Article |
| id | doaj.art-5b87ecc9eb8a439c8a857033c9a04910 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T19:12:13Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-5b87ecc9eb8a439c8a857033c9a049102023-11-24T04:02:49ZengMDPI AGCancers2072-66942022-10-011421533510.3390/cancers14215335Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic FeaturesGiuseppe Roberto Giammalva0Anna Viola1Rosario Maugeri2Kevin Giardina3Rina Di Bonaventura4Sofia Musso5Lara Brunasso6Santiago Cepeda7Giuseppe Maria Della Pepa8Alba Scerrati9Giorgio Mantovani10Gianluca Ferini11Rosa Maria Gerardi12Maria Angela Pino13Giuseppe Emmanuele Umana14Luca Denaro15Alessio Albanese16Domenico Gerardo Iacopino17Neurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyDepartment of Radiation Oncology, REM Radioterapia srl, 95029 Viagrande, ItalyNeurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyNeurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyDepartment of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, Università Cattolica del Sacro Cuore, 00100 Rome, ItalyNeurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyNeurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyDepartamento de Neurocirugía, Hospital Universitario Río Hortega, 47012 Valladolid, SpainDepartment of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, Università Cattolica del Sacro Cuore, 00100 Rome, ItalyDepartment of Translational Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Translational Medicine, University of Ferrara, 44121 Ferrara, ItalyDepartment of Radiation Oncology, REM Radioterapia srl, 95029 Viagrande, ItalyNeurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyNeurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyTrauma Center, Gamma Knife Center, Department of Neurosurgery, Cannizzaro Hospital, 95126 Catania, ItalyAcademic Neurosurgery, Department of Neurosciences DNS, University of Padua, 35128 Padua, ItalyDepartment of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, Università Cattolica del Sacro Cuore, 00100 Rome, ItalyNeurosurgical Clinic, Post Graduate Residency Program in Neurologic Surgery, Department of Biomedicine Neurosciences and Advanced Diagnostics, School of Medicine, University of Palermo, 90127 Palermo, ItalyMicrovascular Doppler (MicroV) is a new-generation Doppler technique developed by Esaote (Esaote s.p.a., Genova, Italy), which is able to visualize small and low-flow vessels through a suppression of interfering signals. MicroV uses advanced filters that are able to differentiate tissue artifacts from low-speed blood flows; by exploiting the space–time coherence information, these filters can selectively suppress tissue components, preserving the signal coming from the microvascular flow. This technique is clinically applied to the study of the vascularization of parenchymatous lesions, often with better diagnostic accuracy than color/power Doppler techniques. The aim of this paper is to develop a reproducible protocol for the recording and collection of MicroV intraoperative ultrasound images by the use of a capable intraoperative ultrasound machine and post-processing aimed at evaluation of brain-tumor microvascularization through the analysis of radiomic features. The proposed protocol has been internally validated on eight patients and will be firstly applied to patients affected by WHO grade IV astrocytoma (glioblastoma—GBM) candidates for craniotomy and lesion removal. In a further stage, it will be generally applied to patients with primary or metastatic brain tumors. IOUS is performed before durotomy. Tumor microvascularization is evaluated using the MicroV Doppler technique and IOUS images are recorded, stored, and post-processed. IOUS images are remotely stored on the BraTIoUS database, which will promote international cooperation and multicentric analysis. Processed images and texture radiomic features are analyzed post-operatively using ImageJ, a free scientific image-analysis software based on the Sun-Java platform. Post-processing protocol is further described in-depth. The study of tumor microvascularization through advanced IOUS techniques such as MicroV could represent, in the future, a non-invasive and real-time method for intraoperative predictive evaluation of the tumor features. This evaluation could finally result in a deeper knowledge of brain-tumor behavior and in the on-going adaptation of the surgery with the improvement of surgical outcomes.https://www.mdpi.com/2072-6694/14/21/5335IOUSmicrovascularizationbrain tumorMicroVprotocolradiomics |
| spellingShingle | Giuseppe Roberto Giammalva Anna Viola Rosario Maugeri Kevin Giardina Rina Di Bonaventura Sofia Musso Lara Brunasso Santiago Cepeda Giuseppe Maria Della Pepa Alba Scerrati Giorgio Mantovani Gianluca Ferini Rosa Maria Gerardi Maria Angela Pino Giuseppe Emmanuele Umana Luca Denaro Alessio Albanese Domenico Gerardo Iacopino Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic Features Cancers IOUS microvascularization brain tumor MicroV protocol radiomics |
| title | Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic Features |
| title_full | Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic Features |
| title_fullStr | Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic Features |
| title_full_unstemmed | Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic Features |
| title_short | Intraoperative Evaluation of Brain-Tumor Microvascularization through MicroV IOUS: A Protocol for Image Acquisition and Analysis of Radiomic Features |
| title_sort | intraoperative evaluation of brain tumor microvascularization through microv ious a protocol for image acquisition and analysis of radiomic features |
| topic | IOUS microvascularization brain tumor MicroV protocol radiomics |
| url | https://www.mdpi.com/2072-6694/14/21/5335 |
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