Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid Carcinoma
Thyroid cancer is the most common endocrine malignancy that its incidence hascontinuously increased in recent decades all over the world. Thyroid cancer in Iranis the seventh most common cancer in women and the 14th in men and the 11th mostcommon cancer in both genders. The study on the methylation...
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| Format: | Article |
| Language: | English |
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University of Sistan and Baluchestan
2019-12-01
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| Series: | Journal of Epigenetics |
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| Online Access: | https://jep.usb.ac.ir/article_5312_ef591e3f24199bb8626e153d8518d3ae.pdf |
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| author | Gholamabbas Dinarvand seyedeh Zeinab Peighambarzadeh Mehdi Tavana |
| author_facet | Gholamabbas Dinarvand seyedeh Zeinab Peighambarzadeh Mehdi Tavana |
| author_sort | Gholamabbas Dinarvand |
| collection | DOAJ |
| description | Thyroid cancer is the most common endocrine malignancy that its incidence hascontinuously increased in recent decades all over the world. Thyroid cancer in Iranis the seventh most common cancer in women and the 14th in men and the 11th mostcommon cancer in both genders. The study on the methylation status of RASSF1Agene promoter has shown that this gene is methylated in 35% of benign andmalignant thyroid tumors. Hypermethylation of the RASSF1A gene use for thedifferentiation of benign tumors from malignant of thyroid gland. The aim of thisstudy was to determine the sensitivity and specificity of DNA methylation ofRASSF1A gene in the differential diagnosis of benign tumors from papillary thyroidcarcinoma.160 samples of patients with malignant thyroid tumors (80 samples) andbenign thyroid (80 samples) were entered into this study from all patients referringto Ahwaz medical centers. The Hypermethylation of the gene after DNA extractionwas done by COBRA method. Finally, for calculating sensitivity and specificity ofthe two tests were done by epidemiological calculations.The sensitivity andspecificity of Hypermethylation of the RASSF1A gene test were 91.25% and 15%respectively.Hypermethylation of the promoter of the RASSF1A gene as a diagnosistest is more sensitive to differential diagnosis of benign tumors from papillarythyroid carcinoma. |
| first_indexed | 2024-03-13T10:46:15Z |
| format | Article |
| id | doaj.art-5b88904dd1b045ada0440746e98bd9d8 |
| institution | Directory Open Access Journal |
| issn | 2676-4350 |
| language | English |
| last_indexed | 2024-03-13T10:46:15Z |
| publishDate | 2019-12-01 |
| publisher | University of Sistan and Baluchestan |
| record_format | Article |
| series | Journal of Epigenetics |
| spelling | doaj.art-5b88904dd1b045ada0440746e98bd9d82023-05-17T18:57:25ZengUniversity of Sistan and BaluchestanJournal of Epigenetics2676-43502019-12-0112172310.22111/jep.2019.30330.10135312Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid CarcinomaGholamabbas Dinarvand0seyedeh Zeinab Peighambarzadeh1Mehdi Tavana2Department of Biochemistry, Shoushtar Branch, Islamic Azad University, Shoushtar, IranDepartment of Veterinary medicine, School of Agriculture, Shoushtar Branch, Islamic Azad University, Shoushtar, IranDepartment of Veterinary medicine, School of Agriculture, Shoushtar Branch, Islamic Azad University, Shoushtar, IranThyroid cancer is the most common endocrine malignancy that its incidence hascontinuously increased in recent decades all over the world. Thyroid cancer in Iranis the seventh most common cancer in women and the 14th in men and the 11th mostcommon cancer in both genders. The study on the methylation status of RASSF1Agene promoter has shown that this gene is methylated in 35% of benign andmalignant thyroid tumors. Hypermethylation of the RASSF1A gene use for thedifferentiation of benign tumors from malignant of thyroid gland. The aim of thisstudy was to determine the sensitivity and specificity of DNA methylation ofRASSF1A gene in the differential diagnosis of benign tumors from papillary thyroidcarcinoma.160 samples of patients with malignant thyroid tumors (80 samples) andbenign thyroid (80 samples) were entered into this study from all patients referringto Ahwaz medical centers. The Hypermethylation of the gene after DNA extractionwas done by COBRA method. Finally, for calculating sensitivity and specificity ofthe two tests were done by epidemiological calculations.The sensitivity andspecificity of Hypermethylation of the RASSF1A gene test were 91.25% and 15%respectively.Hypermethylation of the promoter of the RASSF1A gene as a diagnosistest is more sensitive to differential diagnosis of benign tumors from papillarythyroid carcinoma.https://jep.usb.ac.ir/article_5312_ef591e3f24199bb8626e153d8518d3ae.pdfhypermethylationrassf1a genethyroid papillary carcinomasensitivityspecificity |
| spellingShingle | Gholamabbas Dinarvand seyedeh Zeinab Peighambarzadeh Mehdi Tavana Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid Carcinoma Journal of Epigenetics hypermethylation rassf1a gene thyroid papillary carcinoma sensitivity specificity |
| title | Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid Carcinoma |
| title_full | Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid Carcinoma |
| title_fullStr | Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid Carcinoma |
| title_full_unstemmed | Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid Carcinoma |
| title_short | Diagnostic Value of RASSF1A gene DNA methylation in Differential Diagnosis of Thyroid Benign Tumors and Papillary Thyroid Carcinoma |
| title_sort | diagnostic value of rassf1a gene dna methylation in differential diagnosis of thyroid benign tumors and papillary thyroid carcinoma |
| topic | hypermethylation rassf1a gene thyroid papillary carcinoma sensitivity specificity |
| url | https://jep.usb.ac.ir/article_5312_ef591e3f24199bb8626e153d8518d3ae.pdf |
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