Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months Period
Water samples from two naturally hypersaline lakes, renowned for their balneotherapeutic properties, were investigated through a pilot SERS monitoring program. Nanotechnology-based techniques were employed to periodically measure the ultra-sensitive SERS molecular characteristics of the raw water-be...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/1/19 |
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| author | Csilla Molnár Teodora Diana Drigla Lucian Barbu-Tudoran Ilirjana Bajama Victor Curean Simona Cîntă Pînzaru |
| author_facet | Csilla Molnár Teodora Diana Drigla Lucian Barbu-Tudoran Ilirjana Bajama Victor Curean Simona Cîntă Pînzaru |
| author_sort | Csilla Molnár |
| collection | DOAJ |
| description | Water samples from two naturally hypersaline lakes, renowned for their balneotherapeutic properties, were investigated through a pilot SERS monitoring program. Nanotechnology-based techniques were employed to periodically measure the ultra-sensitive SERS molecular characteristics of the raw water-bearing microbial community and the inorganic content. Employing the Pearson correlation coefficient revealed a robust linear relationship between electrical conductivity and pH and Raman and SERS spectral data of water samples, highlighting the interplay complexity of Raman/SERS signals and physicochemical parameters within each lake. The SERS data obtained from raw waters with AgNPs exhibited a dominant, reproducible SERS feature resembling adsorbed β-carotene at submicromole concentration, which could be related to the cyanobacteria-AgNPs interface and supported by TEM analyses. Notably, spurious SERS sampling cases showed molecular traces attributed to additional metabolites, suggesting multiplexed SERS signatures. The conducted PCA demonstrated observable differences in the β-carotene SERS band intensities between the two lakes, signifying potential variations in picoplankton abundance and composition or environmental influences. Moreover, the study examined variations in the SERS intensity ratio I<sub>245</sub>/I<sub>1512</sub>, related to the balance between inorganic (Cl<sup>−</sup>-induced AgNPs aggregation) and organic (cyanobacteria population) balance, in correlation with the electrical conductivity. These findings signify the potential of SERS data for monitoring variations in microorganism concentration, clearly dependent on ion concentration and nutrient dynamics in raw, hypersaline water bodies. |
| first_indexed | 2024-03-08T11:04:17Z |
| format | Article |
| id | doaj.art-5b8933bd4d9643a68469e1be40b0fb8b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-08T11:04:17Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-5b8933bd4d9643a68469e1be40b0fb8b2024-01-26T15:23:04ZengMDPI AGBiosensors2079-63742023-12-011411910.3390/bios14010019Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months PeriodCsilla Molnár0Teodora Diana Drigla1Lucian Barbu-Tudoran2Ilirjana Bajama3Victor Curean4Simona Cîntă Pînzaru5National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath, 400293 Cluj-Napoca, RomaniaBiomolecular Physics Department, Babeş-Bolyai University, Kogălniceanu 1, 400084 Cluj Napoca, RomaniaElectron Microscopy Centre, Babeș-Bolyai University, Clinicilor 5-7, 400006 Cluj-Napoca, RomaniaBiomolecular Physics Department, Babeş-Bolyai University, Kogălniceanu 1, 400084 Cluj Napoca, RomaniaFaculty of Pharmacy, “Iuliu Hatieganu” University of Medicine and Pharmacy, Victor Babes 8, 400347 Cluj-Napoca, RomaniaBiomolecular Physics Department, Babeş-Bolyai University, Kogălniceanu 1, 400084 Cluj Napoca, RomaniaWater samples from two naturally hypersaline lakes, renowned for their balneotherapeutic properties, were investigated through a pilot SERS monitoring program. Nanotechnology-based techniques were employed to periodically measure the ultra-sensitive SERS molecular characteristics of the raw water-bearing microbial community and the inorganic content. Employing the Pearson correlation coefficient revealed a robust linear relationship between electrical conductivity and pH and Raman and SERS spectral data of water samples, highlighting the interplay complexity of Raman/SERS signals and physicochemical parameters within each lake. The SERS data obtained from raw waters with AgNPs exhibited a dominant, reproducible SERS feature resembling adsorbed β-carotene at submicromole concentration, which could be related to the cyanobacteria-AgNPs interface and supported by TEM analyses. Notably, spurious SERS sampling cases showed molecular traces attributed to additional metabolites, suggesting multiplexed SERS signatures. The conducted PCA demonstrated observable differences in the β-carotene SERS band intensities between the two lakes, signifying potential variations in picoplankton abundance and composition or environmental influences. Moreover, the study examined variations in the SERS intensity ratio I<sub>245</sub>/I<sub>1512</sub>, related to the balance between inorganic (Cl<sup>−</sup>-induced AgNPs aggregation) and organic (cyanobacteria population) balance, in correlation with the electrical conductivity. These findings signify the potential of SERS data for monitoring variations in microorganism concentration, clearly dependent on ion concentration and nutrient dynamics in raw, hypersaline water bodies.https://www.mdpi.com/2079-6374/14/1/19RamanSERSsalt lakes monitoringbalneary resortsin situPCA |
| spellingShingle | Csilla Molnár Teodora Diana Drigla Lucian Barbu-Tudoran Ilirjana Bajama Victor Curean Simona Cîntă Pînzaru Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months Period Biosensors Raman SERS salt lakes monitoring balneary resorts in situ PCA |
| title | Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months Period |
| title_full | Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months Period |
| title_fullStr | Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months Period |
| title_full_unstemmed | Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months Period |
| title_short | Pilot SERS Monitoring Study of Two Natural Hypersaline Lake Waters from a Balneary Resort during Winter-Months Period |
| title_sort | pilot sers monitoring study of two natural hypersaline lake waters from a balneary resort during winter months period |
| topic | Raman SERS salt lakes monitoring balneary resorts in situ PCA |
| url | https://www.mdpi.com/2079-6374/14/1/19 |
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