Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis
Although human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged ex vivo time required to develop an implantable device. Considering that macromolecular crowding has the potential to substantially accelera...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642400036X |
| _version_ | 1827315542632955904 |
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| author | Adrian Djalali-Cuevas Mandy Rettel Frank Stein Mikhail Savitski Stephen Kearns Jack Kelly Manus Biggs Ioannis Skoufos Athina Tzora Nikitas Prassinos Nikolaos Diakakis Dimitrios I. Zeugolis |
| author_facet | Adrian Djalali-Cuevas Mandy Rettel Frank Stein Mikhail Savitski Stephen Kearns Jack Kelly Manus Biggs Ioannis Skoufos Athina Tzora Nikitas Prassinos Nikolaos Diakakis Dimitrios I. Zeugolis |
| author_sort | Adrian Djalali-Cuevas |
| collection | DOAJ |
| description | Although human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged ex vivo time required to develop an implantable device. Considering that macromolecular crowding has the potential to substantially accelerate the development of functional tissue facsimiles, herein we compared human tenocyte and dermal fibroblast behaviour under standard and macromolecular crowding conditions to inform future studies in tendon engineering. Basic cell function analysis made apparent the innocuousness of macromolecular crowding for both cell types. Gene expression analysis of the without macromolecular crowding groups revealed expression of tendon related molecules in human dermal fibroblasts and tenocytes. Protein electrophoresis and immunocytochemistry analyses showed significantly increased and similar deposition of collagen fibres by macromolecular crowding in the two cell types. Proteomics analysis demonstrated great similarities between human tenocyte and dermal fibroblast cultures, as well as the induction of haemostatic, anti-microbial and tissue-protective proteins by macromolecular crowding in both cell populations. Collectively, these data rationalise the use of either human dermal fibroblasts or tenocytes in combination with macromolecular crowding in tendon engineering. |
| first_indexed | 2024-03-08T09:01:29Z |
| format | Article |
| id | doaj.art-5b8a4a72beb64a32962082aa003501bd |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-24T22:57:01Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-5b8a4a72beb64a32962082aa003501bd2024-03-18T04:34:20ZengElsevierMaterials Today Bio2590-00642024-04-0125100977Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysisAdrian Djalali-Cuevas0Mandy Rettel1Frank Stein2Mikhail Savitski3Stephen Kearns4Jack Kelly5Manus Biggs6Ioannis Skoufos7Athina Tzora8Nikitas Prassinos9Nikolaos Diakakis10Dimitrios I. Zeugolis11Laboratory of Animal Science, Nutrition and Biotechnology, School of Agriculture, University of Ioannina, Arta, Greece; School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece; Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Charles Institute of Dermatology, Conway Institute of Biomolecular & Biomedical Research and School of Mechanical & Materials Engineering, University College Dublin (UCD), Dublin, IrelandProteomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, GermanyProteomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, GermanyProteomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, GermanyMerlin Park University Hospital, Galway, IrelandGalway University Hospital, Galway, IrelandScience Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, University of Galway, Galway, IrelandLaboratory of Animal Science, Nutrition and Biotechnology, School of Agriculture, University of Ioannina, Arta, GreeceLaboratory of Animal Science, Nutrition and Biotechnology, School of Agriculture, University of Ioannina, Arta, GreeceSchool of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, GreeceSchool of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, GreeceRegenerative, Modular & Developmental Engineering Laboratory (REMODEL), Charles Institute of Dermatology, Conway Institute of Biomolecular & Biomedical Research and School of Mechanical & Materials Engineering, University College Dublin (UCD), Dublin, Ireland; Corresponding author. REMODEL, UCD, Ireland.Although human tenocytes and dermal fibroblasts have shown promise in tendon engineering, no tissue engineered medicine has been developed due to the prolonged ex vivo time required to develop an implantable device. Considering that macromolecular crowding has the potential to substantially accelerate the development of functional tissue facsimiles, herein we compared human tenocyte and dermal fibroblast behaviour under standard and macromolecular crowding conditions to inform future studies in tendon engineering. Basic cell function analysis made apparent the innocuousness of macromolecular crowding for both cell types. Gene expression analysis of the without macromolecular crowding groups revealed expression of tendon related molecules in human dermal fibroblasts and tenocytes. Protein electrophoresis and immunocytochemistry analyses showed significantly increased and similar deposition of collagen fibres by macromolecular crowding in the two cell types. Proteomics analysis demonstrated great similarities between human tenocyte and dermal fibroblast cultures, as well as the induction of haemostatic, anti-microbial and tissue-protective proteins by macromolecular crowding in both cell populations. Collectively, these data rationalise the use of either human dermal fibroblasts or tenocytes in combination with macromolecular crowding in tendon engineering.http://www.sciencedirect.com/science/article/pii/S259000642400036XTendon engineeringDermal fibroblastsTenocytesMacromolecular crowdingProteomics |
| spellingShingle | Adrian Djalali-Cuevas Mandy Rettel Frank Stein Mikhail Savitski Stephen Kearns Jack Kelly Manus Biggs Ioannis Skoufos Athina Tzora Nikitas Prassinos Nikolaos Diakakis Dimitrios I. Zeugolis Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis Materials Today Bio Tendon engineering Dermal fibroblasts Tenocytes Macromolecular crowding Proteomics |
| title | Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis |
| title_full | Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis |
| title_fullStr | Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis |
| title_full_unstemmed | Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis |
| title_short | Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis |
| title_sort | macromolecular crowding in human tenocyte and skin fibroblast cultures a comparative analysis |
| topic | Tendon engineering Dermal fibroblasts Tenocytes Macromolecular crowding Proteomics |
| url | http://www.sciencedirect.com/science/article/pii/S259000642400036X |
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