<i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets
Climatic variations in the current environmental scenario require plants with tolerance to sudden changes in temperature and a decrease in water availability. Accordingly, this tolerance will enable successful plantations and the maintenance of natural and planted forests. Consequently, in the last...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/11/11/1181 |
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| author | Antonia Maiara Marques do Nascimento Priscila Alves Barroso Naysa Flavia Ferreira do Nascimento Tomás Goicoa María Dolores Ugarte Itziar Aurora Montalbán Paloma Moncaleán |
| author_facet | Antonia Maiara Marques do Nascimento Priscila Alves Barroso Naysa Flavia Ferreira do Nascimento Tomás Goicoa María Dolores Ugarte Itziar Aurora Montalbán Paloma Moncaleán |
| author_sort | Antonia Maiara Marques do Nascimento |
| collection | DOAJ |
| description | Climatic variations in the current environmental scenario require plants with tolerance to sudden changes in temperature and a decrease in water availability. Accordingly, this tolerance will enable successful plantations and the maintenance of natural and planted forests. Consequently, in the last two decades, drought tolerance and high temperatures in conifers have been an important target for morphological, physiological, and epigenetic studies. Based on this, our research team has optimized different stages of somatic embryogenesis (SE) in <i>Pinus</i> spp. improving the success of the process. Through this method, we can obtain a large amount of clonal material and then analyze the somatic plants under different conditions ex vitro. The analysis of the morphological and physiological parameters in somatic embryos (ses) and plants with different tolerances to abiotic stress can provide us with valuable information about the mechanisms used by plants to survive under adverse environmental conditions. Thus, the objective of this work was to evaluate the influence of high temperatures (23, 40, 50, and 60 °C, after 12 weeks, 90, 30, 5 min, respectively) on the morphology of somatic embryos obtained from <i>Pinus radiata</i> D.Don (Radiata pine) and <i>Pinus halepensis</i> Mill. (Aleppo pine). In addition, we carried out a physiological evaluation of the somatic plants of <i>P. radiata</i> submitted to heat and water stress in a greenhouse. We observed that the number of somatic embryos was not affected by maturation temperatures in both species. Likewise, <i>P. radiata</i> plants obtained from these somatic embryos survived drought and heat stress in the greenhouse. In addition, plants originating from embryonal masses (EMs) subjected to high maturation temperature (40 and 60 °C) had a significant increase in <i>g<sub>s</sub></i> and <i>E</i>. Therefore, it is possible to modulate the characteristics of somatic plants produced by the manipulation of environmental conditions during the process of SE. |
| first_indexed | 2024-03-10T15:01:01Z |
| format | Article |
| id | doaj.art-5b8bf57009194c8db7abebd2312a664f |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T15:01:01Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-5b8bf57009194c8db7abebd2312a664f2023-11-20T20:12:30ZengMDPI AGForests1999-49072020-11-011111118110.3390/f11111181<i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of PlantletsAntonia Maiara Marques do Nascimento0Priscila Alves Barroso1Naysa Flavia Ferreira do Nascimento2Tomás Goicoa3María Dolores Ugarte4Itziar Aurora Montalbán5Paloma Moncaleán6Departamento de Ciencias Forestales, Neiker-BRTA, 01080 Arkaute, SpainDepartamento de Agronomía, Campus Professora Cinobelina Elvas, Universidade Federal do Piauí, Bom Jesus, Piauí 64900-000, BrazilDepartamento de Fitotecnia e Ciências Ambientais, Universidade Federal da Paraíba, Areia, Paraíba 58397-000, BrazilDepartamento de Estadística, Informática y Matemáticas, Universidad Pública de Navarra, 31006 Pamplona, SpainDepartamento de Estadística, Informática y Matemáticas, Universidad Pública de Navarra, 31006 Pamplona, SpainDepartamento de Ciencias Forestales, Neiker-BRTA, 01080 Arkaute, SpainDepartamento de Ciencias Forestales, Neiker-BRTA, 01080 Arkaute, SpainClimatic variations in the current environmental scenario require plants with tolerance to sudden changes in temperature and a decrease in water availability. Accordingly, this tolerance will enable successful plantations and the maintenance of natural and planted forests. Consequently, in the last two decades, drought tolerance and high temperatures in conifers have been an important target for morphological, physiological, and epigenetic studies. Based on this, our research team has optimized different stages of somatic embryogenesis (SE) in <i>Pinus</i> spp. improving the success of the process. Through this method, we can obtain a large amount of clonal material and then analyze the somatic plants under different conditions ex vitro. The analysis of the morphological and physiological parameters in somatic embryos (ses) and plants with different tolerances to abiotic stress can provide us with valuable information about the mechanisms used by plants to survive under adverse environmental conditions. Thus, the objective of this work was to evaluate the influence of high temperatures (23, 40, 50, and 60 °C, after 12 weeks, 90, 30, 5 min, respectively) on the morphology of somatic embryos obtained from <i>Pinus radiata</i> D.Don (Radiata pine) and <i>Pinus halepensis</i> Mill. (Aleppo pine). In addition, we carried out a physiological evaluation of the somatic plants of <i>P. radiata</i> submitted to heat and water stress in a greenhouse. We observed that the number of somatic embryos was not affected by maturation temperatures in both species. Likewise, <i>P. radiata</i> plants obtained from these somatic embryos survived drought and heat stress in the greenhouse. In addition, plants originating from embryonal masses (EMs) subjected to high maturation temperature (40 and 60 °C) had a significant increase in <i>g<sub>s</sub></i> and <i>E</i>. Therefore, it is possible to modulate the characteristics of somatic plants produced by the manipulation of environmental conditions during the process of SE.https://www.mdpi.com/1999-4907/11/11/1181abiotic stressembryonal masses<i>Pinus halepensis</i><i>Pinus radiata</i>somatic embryogenesissomatic plantlets |
| spellingShingle | Antonia Maiara Marques do Nascimento Priscila Alves Barroso Naysa Flavia Ferreira do Nascimento Tomás Goicoa María Dolores Ugarte Itziar Aurora Montalbán Paloma Moncaleán <i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets Forests abiotic stress embryonal masses <i>Pinus halepensis</i> <i>Pinus radiata</i> somatic embryogenesis somatic plantlets |
| title | <i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets |
| title_full | <i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets |
| title_fullStr | <i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets |
| title_full_unstemmed | <i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets |
| title_short | <i>Pinus</i> spp. Somatic Embryo Conversion under High Temperature: Effect on the Morphological and Physiological Characteristics of Plantlets |
| title_sort | i pinus i spp somatic embryo conversion under high temperature effect on the morphological and physiological characteristics of plantlets |
| topic | abiotic stress embryonal masses <i>Pinus halepensis</i> <i>Pinus radiata</i> somatic embryogenesis somatic plantlets |
| url | https://www.mdpi.com/1999-4907/11/11/1181 |
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