A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry
IntroductionProducts of plant secondary metabolism, such as phenolic compounds, flavonoids, alkaloids, and hormones, play an important role in plant growth, development, stress resistance. The plant family Rubiaceae is extremely diverse and abundant in Central America and contains several economical...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1057645/full |
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| author | Fernanda R. Castro-Moretti Jean-Christophe Cocuron Humberto Castillo-Gonzalez Efrain Escudero-Leyva Efrain Escudero-Leyva Priscila Chaverri Priscila Chaverri Oliveiro Guerreiro-Filho Jason C. Slot Ana Paula Alonso Ana Paula Alonso |
| author_facet | Fernanda R. Castro-Moretti Jean-Christophe Cocuron Humberto Castillo-Gonzalez Efrain Escudero-Leyva Efrain Escudero-Leyva Priscila Chaverri Priscila Chaverri Oliveiro Guerreiro-Filho Jason C. Slot Ana Paula Alonso Ana Paula Alonso |
| author_sort | Fernanda R. Castro-Moretti |
| collection | DOAJ |
| description | IntroductionProducts of plant secondary metabolism, such as phenolic compounds, flavonoids, alkaloids, and hormones, play an important role in plant growth, development, stress resistance. The plant family Rubiaceae is extremely diverse and abundant in Central America and contains several economically important genera, e.g. Coffea and other medicinal plants. These are known for the production of bioactive polyphenols (e.g. caffeine and quinine), which have had major impacts on human society. The overall goal of this study was to develop a high-throughput workflow to identify and quantify plant polyphenols.MethodsFirst, a method was optimized to extract over 40 families of phytochemicals. Then, a high-throughput metabolomic platform has been developed to identify and quantify 184 polyphenols in 15 min.ResultsThe current metabolomics study of secondary metabolites was conducted on leaves from one commercial coffee variety and two wild species that also belong to the Rubiaceae family. Global profiling was performed using liquid chromatography high-resolution time-of-flight mass spectrometry. Features whose abundance was significantly different between coffee species were discriminated using statistical analysis and annotated using spectral databases. The identified features were validated by commercially available standards using our newly developed liquid chromatography tandem mass spectrometry method.DiscussionCaffeine, trigonelline and theobromine were highly abundant in coffee leaves, as expected. Interestingly, wild Rubiaceae leaves had a higher diversity of phytochemicals in comparison to commercial coffee: defense-related molecules, such as phenylpropanoids (e.g., cinnamic acid), the terpenoid gibberellic acid, and the monolignol sinapaldehyde were found more abundantly in wild Rubiaceae leaves. |
| first_indexed | 2024-04-11T00:38:02Z |
| format | Article |
| id | doaj.art-5b8eba8b9e534f6f9f6153313af558be |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T00:38:02Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-5b8eba8b9e534f6f9f6153313af558be2023-01-06T13:34:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10576451057645A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometryFernanda R. Castro-Moretti0Jean-Christophe Cocuron1Humberto Castillo-Gonzalez2Efrain Escudero-Leyva3Efrain Escudero-Leyva4Priscila Chaverri5Priscila Chaverri6Oliveiro Guerreiro-Filho7Jason C. Slot8Ana Paula Alonso9Ana Paula Alonso10BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX, United StatesBioAnalytical Facility, University of North Texas, Denton, TX, United StatesDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, United StatesSchool of Biology and Natural Products Research Center Centro de Investigaciones en Productos Naturales (CIPRONA), University of Costa Rica, San Jose, Costa RicaCentro Nacional de Alta Technologia-Consejo Nacional de Rectores (CeNAT-CONARE), National Center for Biotechnological Innovations (CENIBiot), San Jose, Costa RicaDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, United StatesSchool of Biology and Natural Products Research Center Centro de Investigaciones en Productos Naturales (CIPRONA), University of Costa Rica, San Jose, Costa RicaCoffee Center Agronomic Institute, Campinas, Sao Paulo, BrazilDepartment of Plant Pathology, The Ohio State University, Columbus, OH, United StatesBioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX, United StatesBioAnalytical Facility, University of North Texas, Denton, TX, United StatesIntroductionProducts of plant secondary metabolism, such as phenolic compounds, flavonoids, alkaloids, and hormones, play an important role in plant growth, development, stress resistance. The plant family Rubiaceae is extremely diverse and abundant in Central America and contains several economically important genera, e.g. Coffea and other medicinal plants. These are known for the production of bioactive polyphenols (e.g. caffeine and quinine), which have had major impacts on human society. The overall goal of this study was to develop a high-throughput workflow to identify and quantify plant polyphenols.MethodsFirst, a method was optimized to extract over 40 families of phytochemicals. Then, a high-throughput metabolomic platform has been developed to identify and quantify 184 polyphenols in 15 min.ResultsThe current metabolomics study of secondary metabolites was conducted on leaves from one commercial coffee variety and two wild species that also belong to the Rubiaceae family. Global profiling was performed using liquid chromatography high-resolution time-of-flight mass spectrometry. Features whose abundance was significantly different between coffee species were discriminated using statistical analysis and annotated using spectral databases. The identified features were validated by commercially available standards using our newly developed liquid chromatography tandem mass spectrometry method.DiscussionCaffeine, trigonelline and theobromine were highly abundant in coffee leaves, as expected. Interestingly, wild Rubiaceae leaves had a higher diversity of phytochemicals in comparison to commercial coffee: defense-related molecules, such as phenylpropanoids (e.g., cinnamic acid), the terpenoid gibberellic acid, and the monolignol sinapaldehyde were found more abundantly in wild Rubiaceae leaves.https://www.frontiersin.org/articles/10.3389/fpls.2022.1057645/fullLC-MS/MSphenolicsphytochemicalsRubiaceaesecondary metabolism |
| spellingShingle | Fernanda R. Castro-Moretti Jean-Christophe Cocuron Humberto Castillo-Gonzalez Efrain Escudero-Leyva Efrain Escudero-Leyva Priscila Chaverri Priscila Chaverri Oliveiro Guerreiro-Filho Jason C. Slot Ana Paula Alonso Ana Paula Alonso A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry Frontiers in Plant Science LC-MS/MS phenolics phytochemicals Rubiaceae secondary metabolism |
| title | A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry |
| title_full | A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry |
| title_fullStr | A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry |
| title_full_unstemmed | A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry |
| title_short | A metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry |
| title_sort | metabolomic platform to identify and quantify polyphenols in coffee and related species using liquid chromatography mass spectrometry |
| topic | LC-MS/MS phenolics phytochemicals Rubiaceae secondary metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1057645/full |
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