Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMR
This review presents the results of studies of molecular exchange processes in various biological systems (erythrocytes, yeast, liposomes, etc.) performed using pulsed field gradient NMR (PFG NMR). The main theory of processing necessary for the analysis of experimental data is briefly presented: th...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/13/6/567 |
| _version_ | 1797593579482251264 |
|---|---|
| author | Irina A. Avilova Vitaly I. Volkov |
| author_facet | Irina A. Avilova Vitaly I. Volkov |
| author_sort | Irina A. Avilova |
| collection | DOAJ |
| description | This review presents the results of studies of molecular exchange processes in various biological systems (erythrocytes, yeast, liposomes, etc.) performed using pulsed field gradient NMR (PFG NMR). The main theory of processing necessary for the analysis of experimental data is briefly presented: the extraction of self-diffusion coefficients, calculation of cell sizes, and permeability of cell membranes. Attention is paid to the results of assessing the permeability of biological membranes for water molecules and biologically active compounds. The results for other systems are also presented: yeast, chlorella, and plant cells. The results of studies of the lateral diffusion of lipid and cholesterol molecules in model bilayers are also presented. |
| first_indexed | 2024-03-11T02:10:14Z |
| format | Article |
| id | doaj.art-5b92cfcd7b7e4ee990719b3cfb902e21 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-11T02:10:14Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-5b92cfcd7b7e4ee990719b3cfb902e212023-11-18T11:33:25ZengMDPI AGMembranes2077-03752023-05-0113656710.3390/membranes13060567Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMRIrina A. Avilova0Vitaly I. Volkov1Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry RAS, 142432 Chernogolovka, RussiaFederal Research Center of Problems of Chemical Physics and Medicinal Chemistry RAS, 142432 Chernogolovka, RussiaThis review presents the results of studies of molecular exchange processes in various biological systems (erythrocytes, yeast, liposomes, etc.) performed using pulsed field gradient NMR (PFG NMR). The main theory of processing necessary for the analysis of experimental data is briefly presented: the extraction of self-diffusion coefficients, calculation of cell sizes, and permeability of cell membranes. Attention is paid to the results of assessing the permeability of biological membranes for water molecules and biologically active compounds. The results for other systems are also presented: yeast, chlorella, and plant cells. The results of studies of the lateral diffusion of lipid and cholesterol molecules in model bilayers are also presented.https://www.mdpi.com/2077-0375/13/6/567biological cellbiological membranepulsed field gradient NMRdiffusionpermeabilitylipid bilayers |
| spellingShingle | Irina A. Avilova Vitaly I. Volkov Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMR Membranes biological cell biological membrane pulsed field gradient NMR diffusion permeability lipid bilayers |
| title | Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMR |
| title_full | Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMR |
| title_fullStr | Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMR |
| title_full_unstemmed | Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMR |
| title_short | Water and Molecular Exchange in Biological Cells Studied Using <sup>1</sup>H Pulsed Field Gradient NMR |
| title_sort | water and molecular exchange in biological cells studied using sup 1 sup h pulsed field gradient nmr |
| topic | biological cell biological membrane pulsed field gradient NMR diffusion permeability lipid bilayers |
| url | https://www.mdpi.com/2077-0375/13/6/567 |
| work_keys_str_mv | AT irinaaavilova waterandmolecularexchangeinbiologicalcellsstudiedusingsup1suphpulsedfieldgradientnmr AT vitalyivolkov waterandmolecularexchangeinbiologicalcellsstudiedusingsup1suphpulsedfieldgradientnmr |