Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae
The macroalgae surface allows specific bacterial communities to colonize, resulting in complex biological interactions. In recent years, several researchers have studied the diversity and function of the epiphytic bacteria associated with algal host, but largely these interactions remain underexplor...
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/12/2513 |
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| author | Pravin Kumar Ashish Verma Shiva S. Sundharam Anup Kumar Ojha Srinivasan Krishnamurthi |
| author_facet | Pravin Kumar Ashish Verma Shiva S. Sundharam Anup Kumar Ojha Srinivasan Krishnamurthi |
| author_sort | Pravin Kumar |
| collection | DOAJ |
| description | The macroalgae surface allows specific bacterial communities to colonize, resulting in complex biological interactions. In recent years, several researchers have studied the diversity and function of the epiphytic bacteria associated with algal host, but largely these interactions remain underexplored. In the present study we analysed the cultivable diversity and polymer degradation potential of epiphytic bacteria associated with five different marine macroalgae (<i>Sargassum</i>, <i>Ulva</i>, <i>Padina</i>, <i>Dictyota</i> and <i>Pterocladia</i> sp.) sampled from the central west coast of India. Out of the total 360 strains isolated, purified and preserved, about 238 strains were identified through 16S rRNA gene sequence analysis and processed for polymer (cellulose, pectin, xylan and starch) degrading activities. Phylogeny placed the strains within the classes <i>Actinobacteria</i>, <i>Bacilli, Alpha-proteobacteria,</i> and <i>Gamma-proteobacteria</i> and clustered them into 45 genera, wherein <i>Vibrio</i>, <i>Bacillus</i>, <i>Pseudoalteromonas</i>, <i>Alteromonas, Staphylococcus</i> and <i>Kocuria</i> spp. were the most abundant with 20 strains identified as potentially novel taxa within the genera <i>Bacillus, Cellulosimicrobium, Gordonia, Marinomonas, Vibrio, Luteimonas</i> and <i>Pseudoalteromonas</i>. In terms of polymer hydrolysis potential, 61.3% had xylanase activity, while 59.7%, 58.8%, and 52.2% had amylase, cellulase, and pectinase activity, respectively. Overall, 75.6% of the strains degraded more than one polysaccharide, 24% degraded all polymers, while nine strains (3.8%) degraded raw sugarcane bagasse. This study showed great potential for seaweed-associated bacteria in the bio-remediation of agro-waste based raw materials, which can be employed in the form of green technology. |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:04:11Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-5b9423d957064fe9bdd7c07ad3909d512023-11-24T16:50:22ZengMDPI AGMicroorganisms2076-26072022-12-011012251310.3390/microorganisms10122513Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine MacroalgaePravin Kumar0Ashish Verma1Shiva S. Sundharam2Anup Kumar Ojha3Srinivasan Krishnamurthi4Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, IndiaMicrobial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh 160036, IndiaThe macroalgae surface allows specific bacterial communities to colonize, resulting in complex biological interactions. In recent years, several researchers have studied the diversity and function of the epiphytic bacteria associated with algal host, but largely these interactions remain underexplored. In the present study we analysed the cultivable diversity and polymer degradation potential of epiphytic bacteria associated with five different marine macroalgae (<i>Sargassum</i>, <i>Ulva</i>, <i>Padina</i>, <i>Dictyota</i> and <i>Pterocladia</i> sp.) sampled from the central west coast of India. Out of the total 360 strains isolated, purified and preserved, about 238 strains were identified through 16S rRNA gene sequence analysis and processed for polymer (cellulose, pectin, xylan and starch) degrading activities. Phylogeny placed the strains within the classes <i>Actinobacteria</i>, <i>Bacilli, Alpha-proteobacteria,</i> and <i>Gamma-proteobacteria</i> and clustered them into 45 genera, wherein <i>Vibrio</i>, <i>Bacillus</i>, <i>Pseudoalteromonas</i>, <i>Alteromonas, Staphylococcus</i> and <i>Kocuria</i> spp. were the most abundant with 20 strains identified as potentially novel taxa within the genera <i>Bacillus, Cellulosimicrobium, Gordonia, Marinomonas, Vibrio, Luteimonas</i> and <i>Pseudoalteromonas</i>. In terms of polymer hydrolysis potential, 61.3% had xylanase activity, while 59.7%, 58.8%, and 52.2% had amylase, cellulase, and pectinase activity, respectively. Overall, 75.6% of the strains degraded more than one polysaccharide, 24% degraded all polymers, while nine strains (3.8%) degraded raw sugarcane bagasse. This study showed great potential for seaweed-associated bacteria in the bio-remediation of agro-waste based raw materials, which can be employed in the form of green technology.https://www.mdpi.com/2076-2607/10/12/2513macroalgaeepiphytic bacteria16S rRNA gene sequencingpolymer degradationsugarcane bagasse hydrolysis |
| spellingShingle | Pravin Kumar Ashish Verma Shiva S. Sundharam Anup Kumar Ojha Srinivasan Krishnamurthi Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae Microorganisms macroalgae epiphytic bacteria 16S rRNA gene sequencing polymer degradation sugarcane bagasse hydrolysis |
| title | Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae |
| title_full | Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae |
| title_fullStr | Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae |
| title_full_unstemmed | Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae |
| title_short | Exploring Diversity and Polymer Degrading Potential of Epiphytic Bacteria Isolated from Marine Macroalgae |
| title_sort | exploring diversity and polymer degrading potential of epiphytic bacteria isolated from marine macroalgae |
| topic | macroalgae epiphytic bacteria 16S rRNA gene sequencing polymer degradation sugarcane bagasse hydrolysis |
| url | https://www.mdpi.com/2076-2607/10/12/2513 |
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