Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania
Healthcare-associated infections (HCAIs) caused by extended spectrum β-lactamase-producing Gram-negative bacteria (ESBL-GNB) increase morbidity and mortality. This cross-sectional study characterised ESBL genes (blaCTX-M, blaTEM and blaSHV) among 30 ceftriaxone-resistant GNB causing HCAIs between Ja...
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| Format: | Article |
| Language: | English |
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AOSIS
2023-04-01
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| Series: | African Journal of Laboratory Medicine |
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| Online Access: | https://ajlmonline.org/index.php/ajlm/article/view/2107 |
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| author | Jenipher G. Mwakyabala Conjester I. Mtemisika Stacy Mshana Adam A. Mwakyoma Vitus Silago |
| author_facet | Jenipher G. Mwakyabala Conjester I. Mtemisika Stacy Mshana Adam A. Mwakyoma Vitus Silago |
| author_sort | Jenipher G. Mwakyabala |
| collection | DOAJ |
| description | Healthcare-associated infections (HCAIs) caused by extended spectrum β-lactamase-producing Gram-negative bacteria (ESBL-GNB) increase morbidity and mortality. This cross-sectional study characterised ESBL genes (blaCTX-M, blaTEM and blaSHV) among 30 ceftriaxone-resistant GNB causing HCAIs between January 2022 and July 2022 by multiplex polymerase chain reaction assay at the zonal referral hospital in Mwanza, Tanzania. Twenty-five (83.3%) had at least one ESBL gene, of which 23/25 (92.0%) carried the blaCTX-M gene. Seventy-two percent (18/25) of the GNB-ESBL isolates carried more than one ESBL gene, of which the majority (88.8%; n = 16/25) carried the blaCTX-M and blaTEM genes. Extended spectrum β-lactamase genes, particularly blaCTX-M, are common among ceftriaxone-resistant GNB causing HCAIs.
What this study adds: This study revealed the distribution of genes (blaCTX-M, blaTEM and blaSHV) coding for ESBL production among ceftriaxone resistant GNB causing HCAIs However, all ESBL producing GNB were susceptible towards ceftriaxone-sulbactam indicating that ceftriaxone-sulbactam may be empirically prescribed for treating patients with HCAIs. |
| first_indexed | 2024-04-09T14:37:52Z |
| format | Article |
| id | doaj.art-5b981d6d8a254ec288646dd12e1fbbee |
| institution | Directory Open Access Journal |
| issn | 2225-2002 2225-2010 |
| language | English |
| last_indexed | 2024-04-09T14:37:52Z |
| publishDate | 2023-04-01 |
| publisher | AOSIS |
| record_format | Article |
| series | African Journal of Laboratory Medicine |
| spelling | doaj.art-5b981d6d8a254ec288646dd12e1fbbee2023-05-03T11:25:13ZengAOSISAfrican Journal of Laboratory Medicine2225-20022225-20102023-04-01121e1e510.4102/ajlm.v12i1.2107426Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, TanzaniaJenipher G. Mwakyabala0Conjester I. Mtemisika1Stacy Mshana2Adam A. Mwakyoma3Vitus Silago4Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, United Republic of TanzaniaMolecular Biology Laboratory, Central Pathology Laboratory, Bugando Medical Centre, Mwanza, United Republic of TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, United Republic of TanzaniaDepartment of Clinical Microbiology, Kilimanjaro Christian Medical Centre, Moshi, United Republic of TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, United Republic of TanzaniaHealthcare-associated infections (HCAIs) caused by extended spectrum β-lactamase-producing Gram-negative bacteria (ESBL-GNB) increase morbidity and mortality. This cross-sectional study characterised ESBL genes (blaCTX-M, blaTEM and blaSHV) among 30 ceftriaxone-resistant GNB causing HCAIs between January 2022 and July 2022 by multiplex polymerase chain reaction assay at the zonal referral hospital in Mwanza, Tanzania. Twenty-five (83.3%) had at least one ESBL gene, of which 23/25 (92.0%) carried the blaCTX-M gene. Seventy-two percent (18/25) of the GNB-ESBL isolates carried more than one ESBL gene, of which the majority (88.8%; n = 16/25) carried the blaCTX-M and blaTEM genes. Extended spectrum β-lactamase genes, particularly blaCTX-M, are common among ceftriaxone-resistant GNB causing HCAIs. What this study adds: This study revealed the distribution of genes (blaCTX-M, blaTEM and blaSHV) coding for ESBL production among ceftriaxone resistant GNB causing HCAIs However, all ESBL producing GNB were susceptible towards ceftriaxone-sulbactam indicating that ceftriaxone-sulbactam may be empirically prescribed for treating patients with HCAIs.https://ajlmonline.org/index.php/ajlm/article/view/2107beta lactamasesgram-negative bacteriahealthcare associated infectionsmultiplex pcr assay |
| spellingShingle | Jenipher G. Mwakyabala Conjester I. Mtemisika Stacy Mshana Adam A. Mwakyoma Vitus Silago Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania African Journal of Laboratory Medicine beta lactamases gram-negative bacteria healthcare associated infections multiplex pcr assay |
| title | Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania |
| title_full | Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania |
| title_fullStr | Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania |
| title_full_unstemmed | Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania |
| title_short | Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania |
| title_sort | characterisation of genes encoding for extended spectrum β lactamase in gram negative bacteria causing healthcare associated infections in mwanza tanzania |
| topic | beta lactamases gram-negative bacteria healthcare associated infections multiplex pcr assay |
| url | https://ajlmonline.org/index.php/ajlm/article/view/2107 |
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