Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes
Reniform nematode (<i>Rotylenchulus reniformis</i>) is an important microparasite for Upland cotton (<i>Gossypium hirsutum</i> L.) production. Growing resistant cultivars is the most economical management method, but only a few <i>G. barbadense</i> genotypes and s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/7/958 |
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| author | Chunda Feng Salliana R. Stetina John E. Erpelding |
| author_facet | Chunda Feng Salliana R. Stetina John E. Erpelding |
| author_sort | Chunda Feng |
| collection | DOAJ |
| description | Reniform nematode (<i>Rotylenchulus reniformis</i>) is an important microparasite for Upland cotton (<i>Gossypium hirsutum</i> L.) production. Growing resistant cultivars is the most economical management method, but only a few <i>G. barbadense</i> genotypes and some diploid Gossypium species confer high levels of resistance. This study conducted a transcriptome analysis of resistant genotypes to identify genes involved in host plant defense. Seedlings of <i>G. arboreum</i> accessions PI 529728 (A2-100) and PI 615699 (A2-190), and <i>G. barbadense</i> genotypes PI 608139 (GB 713) and PI 163608 (TX 110), were inoculated with the reniform nematode population MSRR04 and root samples were collected on the fifth (D5) and ninth (D9) day after inoculation. Differentially expressed genes (DEGs) were identified by comparing root transcriptomes from inoculated plants with those from non-inoculated plants. Accessions A2-100 and A2-190 showed 52 and 29 DEGs on D5, respectively, with 14 DEGs in common, and 18 DEGs for A2-100 and 11 DEGs for A2-190 on chromosome 5. On D9, four DEGs were found in A2-100 and two DEGs in A2-190. For GB 713, 52 and 43 DEGs were found, and for TX 110, 29 and 117 DEGs were observed on D5 and D9, respectively. Six DEGs were common at the two sampling times for these genotypes. Some DEGs were identified as Meloidogyne-induced cotton (<i>MIC</i>) 3 and 4, resistance gene analogs, or receptor-like proteins. Other DEGs have potential roles in plant defense, such as peroxidases, programmed cell death, pathogenesis related proteins, and systemic acquired resistance. Further research on these DEGs will aid in understanding the mechanisms of resistance to explore new applications for the development of resistant cultivars. |
| first_indexed | 2024-04-24T10:36:43Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-24T10:36:43Z |
| publishDate | 2024-03-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-5b98382ed4b142a1a58199d010516a052024-04-12T13:24:44ZengMDPI AGPlants2223-77472024-03-0113795810.3390/plants13070958Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform NematodesChunda Feng0Salliana R. Stetina1John E. Erpelding2USDA Agricultural Research Service, Crop Genetics Research Unit, Stoneville, MS 38776, USAUSDA Agricultural Research Service, Crop Genetics Research Unit, Stoneville, MS 38776, USAUSDA Agricultural Research Service, Crop Genetics Research Unit, Stoneville, MS 38776, USAReniform nematode (<i>Rotylenchulus reniformis</i>) is an important microparasite for Upland cotton (<i>Gossypium hirsutum</i> L.) production. Growing resistant cultivars is the most economical management method, but only a few <i>G. barbadense</i> genotypes and some diploid Gossypium species confer high levels of resistance. This study conducted a transcriptome analysis of resistant genotypes to identify genes involved in host plant defense. Seedlings of <i>G. arboreum</i> accessions PI 529728 (A2-100) and PI 615699 (A2-190), and <i>G. barbadense</i> genotypes PI 608139 (GB 713) and PI 163608 (TX 110), were inoculated with the reniform nematode population MSRR04 and root samples were collected on the fifth (D5) and ninth (D9) day after inoculation. Differentially expressed genes (DEGs) were identified by comparing root transcriptomes from inoculated plants with those from non-inoculated plants. Accessions A2-100 and A2-190 showed 52 and 29 DEGs on D5, respectively, with 14 DEGs in common, and 18 DEGs for A2-100 and 11 DEGs for A2-190 on chromosome 5. On D9, four DEGs were found in A2-100 and two DEGs in A2-190. For GB 713, 52 and 43 DEGs were found, and for TX 110, 29 and 117 DEGs were observed on D5 and D9, respectively. Six DEGs were common at the two sampling times for these genotypes. Some DEGs were identified as Meloidogyne-induced cotton (<i>MIC</i>) 3 and 4, resistance gene analogs, or receptor-like proteins. Other DEGs have potential roles in plant defense, such as peroxidases, programmed cell death, pathogenesis related proteins, and systemic acquired resistance. Further research on these DEGs will aid in understanding the mechanisms of resistance to explore new applications for the development of resistant cultivars.https://www.mdpi.com/2223-7747/13/7/958Gossypium<i>Rotylenchulus reniformis</i>gene expressionresistance genescotton germplasm |
| spellingShingle | Chunda Feng Salliana R. Stetina John E. Erpelding Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes Plants Gossypium <i>Rotylenchulus reniformis</i> gene expression resistance genes cotton germplasm |
| title | Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes |
| title_full | Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes |
| title_fullStr | Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes |
| title_full_unstemmed | Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes |
| title_short | Transcriptome Analysis of Resistant Cotton Germplasm Responding to Reniform Nematodes |
| title_sort | transcriptome analysis of resistant cotton germplasm responding to reniform nematodes |
| topic | Gossypium <i>Rotylenchulus reniformis</i> gene expression resistance genes cotton germplasm |
| url | https://www.mdpi.com/2223-7747/13/7/958 |
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