Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment
Heterakidosis is a parasitic infection in birds caused by the cecal parasite <i>Heterakis</i> spp. The most common species in geese is <i>H. dispar</i>, the largest avian heterakids species. Because of a scarcity of data concerning the <i>H. dispar</i> population,...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/12/7/926 |
| _version_ | 1797440607460786176 |
|---|---|
| author | Kamila Bobrek Andrzej Gaweł Joanna Urbanowicz |
| author_facet | Kamila Bobrek Andrzej Gaweł Joanna Urbanowicz |
| author_sort | Kamila Bobrek |
| collection | DOAJ |
| description | Heterakidosis is a parasitic infection in birds caused by the cecal parasite <i>Heterakis</i> spp. The most common species in geese is <i>H. dispar</i>, the largest avian heterakids species. Because of a scarcity of data concerning the <i>H. dispar</i> population, the aim of this study was the genetic analysis of <i>Heterakis dispar</i> isolated from geese flocks based on the ITS1-5.8rRNA-ITS2 fragment. Among the 71 <i>H. dispar</i> specimens isolated from 20 geese flocks, six haplotypes were determined (A, B, C, D, E, and F). The four nucleotide substitutions were noted in both ITS fragments, and all of them were transitions between adenine and guanine, or thymine and cytosine. The most frequently noted haplotype was type A (45%), followed by type B (18.3%), type C and D (11.3%), type E (8.5%), and F (5.6%). Infection with nematodes from different haplotype groups was noted in 30% of the flocks, with type A being the most prevalent, followed by types B, D, or E to make up 100%. This study represents the first <i>H. dispar</i> population analysis based on the ITS1-5.8rRNA-ITS2 fragment. |
| first_indexed | 2024-03-09T12:10:40Z |
| format | Article |
| id | doaj.art-5b9a75a006c54ef38af9f3e3d200c25d |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T12:10:40Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-5b9a75a006c54ef38af9f3e3d200c25d2023-11-30T22:52:20ZengMDPI AGAnimals2076-26152022-04-0112792610.3390/ani12070926Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 FragmentKamila Bobrek0Andrzej Gaweł1Joanna Urbanowicz2Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Grunwaldzki 45, 50-366 Wrocław, PolandDepartment of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Grunwaldzki 45, 50-366 Wrocław, PolandDepartment of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Grunwaldzki 45, 50-366 Wrocław, PolandHeterakidosis is a parasitic infection in birds caused by the cecal parasite <i>Heterakis</i> spp. The most common species in geese is <i>H. dispar</i>, the largest avian heterakids species. Because of a scarcity of data concerning the <i>H. dispar</i> population, the aim of this study was the genetic analysis of <i>Heterakis dispar</i> isolated from geese flocks based on the ITS1-5.8rRNA-ITS2 fragment. Among the 71 <i>H. dispar</i> specimens isolated from 20 geese flocks, six haplotypes were determined (A, B, C, D, E, and F). The four nucleotide substitutions were noted in both ITS fragments, and all of them were transitions between adenine and guanine, or thymine and cytosine. The most frequently noted haplotype was type A (45%), followed by type B (18.3%), type C and D (11.3%), type E (8.5%), and F (5.6%). Infection with nematodes from different haplotype groups was noted in 30% of the flocks, with type A being the most prevalent, followed by types B, D, or E to make up 100%. This study represents the first <i>H. dispar</i> population analysis based on the ITS1-5.8rRNA-ITS2 fragment.https://www.mdpi.com/2076-2615/12/7/926<i>Heterakis dispar</i>nematodeITS1-5.8rRNA-ITS2geese |
| spellingShingle | Kamila Bobrek Andrzej Gaweł Joanna Urbanowicz Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment Animals <i>Heterakis dispar</i> nematode ITS1-5.8rRNA-ITS2 geese |
| title | Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment |
| title_full | Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment |
| title_fullStr | Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment |
| title_full_unstemmed | Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment |
| title_short | Molecular Analysis of the <i>Heterakis dispar</i> Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment |
| title_sort | molecular analysis of the i heterakis dispar i population in domestic geese based on the its1 5 8rrna its2 fragment |
| topic | <i>Heterakis dispar</i> nematode ITS1-5.8rRNA-ITS2 geese |
| url | https://www.mdpi.com/2076-2615/12/7/926 |
| work_keys_str_mv | AT kamilabobrek molecularanalysisoftheiheterakisdisparipopulationindomesticgeesebasedontheits158rrnaits2fragment AT andrzejgaweł molecularanalysisoftheiheterakisdisparipopulationindomesticgeesebasedontheits158rrnaits2fragment AT joannaurbanowicz molecularanalysisoftheiheterakisdisparipopulationindomesticgeesebasedontheits158rrnaits2fragment |